Strain Fitness in Escherichia coli BW25113 around b2472

Experiment: Putrescine (N)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntacrD and ypfM are separated by 98 nucleotidesypfM and yffB are separated by 380 nucleotidesyffB and dapE are separated by 3 nucleotidesdapE and ypfN are separated by 27 nucleotidesypfN and ypfH are separated by 109 nucleotides b2470: acrD - aminoglycoside/multidrug efflux system (NCBI), at 2,585,617 to 2,588,730 acrD b4606: ypfM - hypothetical protein (RefSeq), at 2,588,829 to 2,588,888 ypfM b2471: yffB - hypothetical protein (NCBI), at 2,589,269 to 2,589,625 yffB b2472: dapE - succinyl-diaminopimelate desuccinylase (NCBI), at 2,589,629 to 2,590,756 dapE b4547: ypfN - hypothetical protein (NCBI), at 2,590,784 to 2,590,984 ypfN b2473: ypfH - orf, hypothetical protein (VIMSS), at 2,591,094 to 2,591,792 ypfH Position (kb) 2589 2590 2591Strain fitness (log2 ratio) -3 -2 -1 0 1at 2588.662 kb on + strandat 2588.682 kb on - strandat 2588.759 kb on - strandat 2588.759 kb on - strandat 2588.790 kb on + strandat 2588.790 kb on + strandat 2588.808 kb on + strandat 2588.808 kb on + strandat 2588.850 kb on + strand, within ypfMat 2588.863 kb on - strand, within ypfMat 2588.865 kb on + strand, within ypfMat 2588.865 kb on + strand, within ypfMat 2588.867 kb on + strand, within ypfMat 2588.868 kb on - strand, within ypfMat 2588.873 kb on + strand, within ypfMat 2588.873 kb on - strand, within ypfMat 2588.873 kb on - strand, within ypfMat 2588.881 kb on - strand, within ypfMat 2588.881 kb on - strand, within ypfMat 2588.888 kb on + strandat 2588.891 kb on - strandat 2588.897 kb on + strandat 2588.897 kb on + strandat 2588.935 kb on - strandat 2588.936 kb on - strandat 2588.937 kb on + strandat 2588.943 kb on - strandat 2588.954 kb on + strandat 2588.967 kb on + strandat 2588.979 kb on - strandat 2588.990 kb on + strandat 2588.990 kb on + strandat 2588.995 kb on + strandat 2589.000 kb on - strandat 2589.000 kb on - strandat 2589.018 kb on + strandat 2589.018 kb on + strandat 2589.020 kb on + strandat 2589.038 kb on - strandat 2589.038 kb on - strandat 2589.050 kb on - strandat 2589.061 kb on - strandat 2589.061 kb on - strandat 2589.061 kb on - strandat 2589.279 kb on - strandat 2589.303 kb on + strandat 2589.332 kb on + strand, within yffBat 2589.332 kb on + strand, within yffBat 2589.360 kb on - strand, within yffBat 2589.360 kb on - strand, within yffBat 2589.360 kb on - strand, within yffBat 2589.376 kb on + strand, within yffBat 2589.376 kb on + strand, within yffBat 2589.443 kb on + strand, within yffBat 2589.447 kb on - strand, within yffBat 2589.453 kb on + strand, within yffBat 2589.624 kb on + strandat 2589.625 kb on + strandat 2589.632 kb on - strandat 2589.641 kb on + strandat 2590.852 kb on + strand, within ypfNat 2590.852 kb on + strand, within ypfNat 2590.934 kb on + strand, within ypfNat 2590.934 kb on + strand, within ypfNat 2590.954 kb on - strand, within ypfNat 2590.954 kb on - strand, within ypfNat 2590.997 kb on + strandat 2591.119 kb on - strandat 2591.119 kb on - strandat 2591.127 kb on - strandat 2591.178 kb on - strand, within ypfHat 2591.178 kb on - strand, within ypfHat 2591.358 kb on - strand, within ypfHat 2591.372 kb on + strand, within ypfHat 2591.372 kb on + strand, within ypfHat 2591.380 kb on - strand, within ypfHat 2591.489 kb on + strand, within ypfHat 2591.494 kb on + strand, within ypfHat 2591.584 kb on - strand, within ypfHat 2591.584 kb on - strand, within ypfHat 2591.680 kb on - strand, within ypfHat 2591.720 kb on - strand, within ypfHat 2591.720 kb on - strand, within ypfHat 2591.752 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Putrescine (N)
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2,588,662 + +0.3
2,588,682 - -0.2
2,588,759 - -0.3
2,588,759 - +0.1
2,588,790 + +0.2
2,588,790 + -0.0
2,588,808 + +1.0
2,588,808 + -0.6
2,588,850 + ypfM b4606 0.35 -0.3
2,588,863 - ypfM b4606 0.57 -0.8
2,588,865 + ypfM b4606 0.60 -1.2
2,588,865 + ypfM b4606 0.60 +0.0
2,588,867 + ypfM b4606 0.63 -1.0
2,588,868 - ypfM b4606 0.65 -0.4
2,588,873 + ypfM b4606 0.73 +0.6
2,588,873 - ypfM b4606 0.73 +0.4
2,588,873 - ypfM b4606 0.73 +0.3
2,588,881 - ypfM b4606 0.87 +0.7
2,588,881 - ypfM b4606 0.87 -0.3
2,588,888 + +0.2
2,588,891 - -1.9
2,588,897 + +0.0
2,588,897 + -0.7
2,588,935 - +0.0
2,588,936 - -0.4
2,588,937 + +0.1
2,588,943 - -0.8
2,588,954 + +0.5
2,588,967 + -1.2
2,588,979 - -0.4
2,588,990 + +0.5
2,588,990 + -0.2
2,588,995 + -0.5
2,589,000 - -0.7
2,589,000 - +0.7
2,589,018 + -0.3
2,589,018 + -0.8
2,589,020 + +0.6
2,589,038 - -0.3
2,589,038 - -0.4
2,589,050 - +0.4
2,589,061 - -0.0
2,589,061 - +0.1
2,589,061 - +0.3
2,589,279 - +0.3
2,589,303 + -0.6
2,589,332 + yffB b2471 0.18 +0.5
2,589,332 + yffB b2471 0.18 +0.0
2,589,360 - yffB b2471 0.25 +0.0
2,589,360 - yffB b2471 0.25 +0.1
2,589,360 - yffB b2471 0.25 -0.0
2,589,376 + yffB b2471 0.30 +0.5
2,589,376 + yffB b2471 0.30 +0.6
2,589,443 + yffB b2471 0.49 +1.2
2,589,447 - yffB b2471 0.50 -0.5
2,589,453 + yffB b2471 0.52 +0.3
2,589,624 + +0.9
2,589,625 + +0.2
2,589,632 - -3.0
2,589,641 + -3.6
2,590,852 + ypfN b4547 0.34 -0.4
2,590,852 + ypfN b4547 0.34 -0.1
2,590,934 + ypfN b4547 0.75 -0.1
2,590,934 + ypfN b4547 0.75 -0.2
2,590,954 - ypfN b4547 0.85 -0.4
2,590,954 - ypfN b4547 0.85 -0.7
2,590,997 + -0.3
2,591,119 - +0.3
2,591,119 - -0.2
2,591,127 - +0.2
2,591,178 - ypfH b2473 0.12 -0.5
2,591,178 - ypfH b2473 0.12 -0.3
2,591,358 - ypfH b2473 0.38 +0.1
2,591,372 + ypfH b2473 0.40 +0.7
2,591,372 + ypfH b2473 0.40 +0.2
2,591,380 - ypfH b2473 0.41 -0.8
2,591,489 + ypfH b2473 0.57 +0.5
2,591,494 + ypfH b2473 0.57 +0.1
2,591,584 - ypfH b2473 0.70 +0.2
2,591,584 - ypfH b2473 0.70 +0.2
2,591,680 - ypfH b2473 0.84 +0.3
2,591,720 - ypfH b2473 0.90 +0.6
2,591,720 - ypfH b2473 0.90 -0.2
2,591,752 + -0.4

Or see this region's nucleotide sequence