Experiment: Putrescine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt dedA and truA are separated by 82 nucleotides truA and usg overlap by 1 nucleotides usg and pdxB are separated by 65 nucleotides
b2317: dedA - conserved inner membrane protein (NCBI), at 2,432,104 to 2,432,763
dedA
b2318: truA - tRNA pseudouridine synthase A (NCBI), at 2,432,846 to 2,433,658
truA
b2319: usg - hypothetical protein (NCBI), at 2,433,658 to 2,434,671
usg
b2320: pdxB - erythronate-4-phosphate dehydrogenase (NCBI), at 2,434,737 to 2,435,873
pdxB
Position (kb)
2433
2434
2435 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1
2 at 2432.800 kb on + strand at 2432.808 kb on - strand at 2432.808 kb on - strand at 2433.004 kb on + strand, within truA at 2433.141 kb on + strand, within truA at 2433.141 kb on + strand, within truA at 2433.157 kb on + strand, within truA at 2433.160 kb on - strand, within truA at 2433.160 kb on - strand, within truA at 2433.170 kb on - strand, within truA at 2433.363 kb on - strand at 2433.465 kb on - strand, within truA at 2433.465 kb on - strand, within truA at 2433.465 kb on - strand, within truA at 2433.465 kb on - strand, within truA at 2433.600 kb on + strand at 2433.600 kb on + strand at 2433.656 kb on + strand at 2433.664 kb on - strand at 2433.670 kb on + strand at 2433.670 kb on + strand at 2433.678 kb on - strand at 2433.753 kb on - strand at 2433.846 kb on + strand, within usg at 2433.853 kb on - strand, within usg at 2433.853 kb on - strand, within usg at 2433.910 kb on + strand, within usg at 2433.943 kb on + strand, within usg at 2433.952 kb on - strand, within usg at 2433.952 kb on - strand, within usg at 2434.057 kb on + strand, within usg at 2434.167 kb on - strand, within usg at 2434.287 kb on - strand, within usg at 2434.287 kb on - strand, within usg at 2434.339 kb on + strand, within usg at 2434.360 kb on + strand, within usg at 2434.626 kb on - strand at 2434.647 kb on + strand at 2434.647 kb on + strand at 2434.690 kb on - strand at 2434.711 kb on + strand at 2434.761 kb on + strand at 2434.787 kb on + strand at 2434.881 kb on - strand, within pdxB at 2434.881 kb on - strand, within pdxB at 2434.932 kb on + strand, within pdxB at 2434.932 kb on + strand, within pdxB at 2434.999 kb on - strand, within pdxB at 2435.038 kb on - strand, within pdxB at 2435.105 kb on - strand at 2435.110 kb on + strand, within pdxB at 2435.110 kb on + strand, within pdxB at 2435.230 kb on + strand, within pdxB at 2435.230 kb on + strand, within pdxB at 2435.246 kb on + strand, within pdxB at 2435.285 kb on + strand, within pdxB at 2435.324 kb on + strand, within pdxB at 2435.324 kb on + strand, within pdxB at 2435.343 kb on - strand, within pdxB at 2435.511 kb on + strand, within pdxB
Per-strain Table
Position Strand Gene LocusTag Fraction Putrescine (N) remove 2,432,800 + +0.2 2,432,808 - +0.1 2,432,808 - +1.4 2,433,004 + truA b2318 0.19 -1.7 2,433,141 + truA b2318 0.36 +2.0 2,433,141 + truA b2318 0.36 -1.9 2,433,157 + truA b2318 0.38 -2.2 2,433,160 - truA b2318 0.39 -1.5 2,433,160 - truA b2318 0.39 -2.2 2,433,170 - truA b2318 0.40 -0.9 2,433,363 - +0.1 2,433,465 - truA b2318 0.76 -1.6 2,433,465 - truA b2318 0.76 -3.7 2,433,465 - truA b2318 0.76 +0.4 2,433,465 - truA b2318 0.76 -1.2 2,433,600 + -2.5 2,433,600 + -1.8 2,433,656 + -1.0 2,433,664 - +0.2 2,433,670 + -1.6 2,433,670 + -2.7 2,433,678 - -0.5 2,433,753 - +0.5 2,433,846 + usg b2319 0.19 -2.4 2,433,853 - usg b2319 0.19 +0.3 2,433,853 - usg b2319 0.19 +1.4 2,433,910 + usg b2319 0.25 -0.9 2,433,943 + usg b2319 0.28 -0.4 2,433,952 - usg b2319 0.29 +0.8 2,433,952 - usg b2319 0.29 -1.4 2,434,057 + usg b2319 0.39 -2.3 2,434,167 - usg b2319 0.50 +0.4 2,434,287 - usg b2319 0.62 +1.1 2,434,287 - usg b2319 0.62 -0.1 2,434,339 + usg b2319 0.67 -1.7 2,434,360 + usg b2319 0.69 -0.2 2,434,626 - +0.6 2,434,647 + -0.4 2,434,647 + -1.2 2,434,690 - +0.1 2,434,711 + +0.3 2,434,761 + -3.1 2,434,787 + -1.2 2,434,881 - pdxB b2320 0.13 -4.9 2,434,881 - pdxB b2320 0.13 -4.5 2,434,932 + pdxB b2320 0.17 -3.2 2,434,932 + pdxB b2320 0.17 -1.6 2,434,999 - pdxB b2320 0.23 -3.8 2,435,038 - pdxB b2320 0.26 -3.0 2,435,105 - -0.9 2,435,110 + pdxB b2320 0.33 -4.2 2,435,110 + pdxB b2320 0.33 -3.5 2,435,230 + pdxB b2320 0.43 -1.7 2,435,230 + pdxB b2320 0.43 -1.7 2,435,246 + pdxB b2320 0.45 -2.9 2,435,285 + pdxB b2320 0.48 -2.8 2,435,324 + pdxB b2320 0.52 -5.1 2,435,324 + pdxB b2320 0.52 -3.6 2,435,343 - pdxB b2320 0.53 -4.3 2,435,511 + pdxB b2320 0.68 -2.7
Or see this region's nucleotide sequence