Experiment: Putrescine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt amn and yeeN are separated by 342 nucleotides yeeN and asnW are separated by 452 nucleotides asnW and yeeO are separated by 100 nucleotides
b1982: amn - AMP nucleosidase (NCBI), at 2,053,085 to 2,054,539
amn
b1983: yeeN - hypothetical protein (NCBI), at 2,054,882 to 2,055,598
yeeN
b1984: asnW - tRNA-Asn (NCBI), at 2,056,051 to 2,056,126
asnW
b1985: yeeO - predicted multidrug efflux system (RefSeq), at 2,056,227 to 2,057,714
yeeO
Position (kb)
2054
2055
2056 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2053.920 kb on + strand, within amn at 2054.011 kb on + strand, within amn at 2054.045 kb on - strand, within amn at 2054.100 kb on + strand, within amn at 2054.197 kb on + strand, within amn at 2054.197 kb on + strand, within amn at 2054.197 kb on + strand, within amn at 2054.197 kb on + strand, within amn at 2054.197 kb on + strand, within amn at 2054.202 kb on - strand, within amn at 2054.204 kb on - strand, within amn at 2054.206 kb on + strand, within amn at 2054.207 kb on + strand, within amn at 2054.215 kb on - strand, within amn at 2054.272 kb on + strand, within amn at 2054.272 kb on + strand, within amn at 2054.299 kb on - strand, within amn at 2054.318 kb on + strand, within amn at 2054.320 kb on + strand, within amn at 2054.328 kb on + strand, within amn at 2054.328 kb on + strand, within amn at 2054.339 kb on + strand, within amn at 2054.346 kb on + strand, within amn at 2054.346 kb on + strand, within amn at 2054.346 kb on + strand, within amn at 2054.346 kb on + strand, within amn at 2054.346 kb on + strand, within amn at 2054.347 kb on - strand, within amn at 2054.349 kb on - strand, within amn at 2054.351 kb on + strand, within amn at 2054.351 kb on + strand, within amn at 2054.351 kb on + strand, within amn at 2054.359 kb on - strand, within amn at 2054.463 kb on + strand at 2054.473 kb on + strand at 2054.476 kb on + strand at 2054.514 kb on + strand at 2054.514 kb on + strand at 2054.581 kb on + strand at 2054.581 kb on + strand at 2054.612 kb on + strand at 2054.620 kb on + strand at 2054.893 kb on - strand at 2054.893 kb on - strand at 2054.963 kb on + strand, within yeeN at 2054.968 kb on + strand, within yeeN at 2054.985 kb on + strand, within yeeN at 2055.129 kb on + strand, within yeeN at 2055.130 kb on + strand, within yeeN at 2055.139 kb on + strand, within yeeN at 2055.146 kb on + strand, within yeeN at 2055.250 kb on + strand, within yeeN at 2055.262 kb on + strand, within yeeN at 2055.262 kb on + strand, within yeeN at 2055.264 kb on + strand, within yeeN at 2055.264 kb on + strand, within yeeN at 2055.264 kb on + strand, within yeeN at 2055.267 kb on + strand, within yeeN at 2055.277 kb on + strand, within yeeN at 2055.309 kb on + strand, within yeeN at 2055.309 kb on + strand, within yeeN at 2055.354 kb on + strand, within yeeN at 2055.354 kb on + strand, within yeeN at 2055.354 kb on + strand, within yeeN at 2055.364 kb on + strand, within yeeN at 2055.364 kb on + strand, within yeeN at 2055.364 kb on + strand, within yeeN at 2055.412 kb on + strand, within yeeN at 2055.420 kb on - strand, within yeeN at 2055.430 kb on + strand, within yeeN at 2055.430 kb on + strand, within yeeN at 2055.443 kb on + strand, within yeeN at 2055.443 kb on + strand, within yeeN at 2055.454 kb on + strand, within yeeN at 2055.487 kb on + strand, within yeeN at 2055.502 kb on + strand, within yeeN at 2055.522 kb on + strand, within yeeN at 2055.543 kb on + strand at 2055.543 kb on + strand at 2055.543 kb on + strand at 2055.782 kb on - strand at 2055.852 kb on - strand at 2055.857 kb on - strand at 2055.857 kb on - strand at 2055.877 kb on - strand at 2055.887 kb on + strand at 2055.889 kb on + strand at 2055.895 kb on - strand at 2055.895 kb on - strand at 2055.895 kb on - strand at 2055.897 kb on - strand at 2055.930 kb on - strand at 2056.031 kb on - strand at 2056.031 kb on - strand at 2056.032 kb on - strand at 2056.075 kb on - strand, within asnW at 2056.312 kb on - strand at 2056.322 kb on - strand at 2056.322 kb on - strand at 2056.326 kb on + strand at 2056.445 kb on + strand, within yeeO at 2056.480 kb on + strand, within yeeO at 2056.557 kb on + strand, within yeeO
Per-strain Table
Position Strand Gene LocusTag Fraction Putrescine (N) remove 2,053,920 + amn b1982 0.57 +2.0 2,054,011 + amn b1982 0.64 +0.7 2,054,045 - amn b1982 0.66 -0.3 2,054,100 + amn b1982 0.70 +0.9 2,054,197 + amn b1982 0.76 -0.7 2,054,197 + amn b1982 0.76 +0.6 2,054,197 + amn b1982 0.76 +0.9 2,054,197 + amn b1982 0.76 -0.9 2,054,197 + amn b1982 0.76 +1.3 2,054,202 - amn b1982 0.77 +0.8 2,054,204 - amn b1982 0.77 +0.0 2,054,206 + amn b1982 0.77 +0.1 2,054,207 + amn b1982 0.77 +1.6 2,054,215 - amn b1982 0.78 +0.1 2,054,272 + amn b1982 0.82 +0.6 2,054,272 + amn b1982 0.82 +0.6 2,054,299 - amn b1982 0.83 +0.0 2,054,318 + amn b1982 0.85 +0.8 2,054,320 + amn b1982 0.85 +0.3 2,054,328 + amn b1982 0.85 +0.9 2,054,328 + amn b1982 0.85 +1.6 2,054,339 + amn b1982 0.86 +1.0 2,054,346 + amn b1982 0.87 +0.0 2,054,346 + amn b1982 0.87 +1.5 2,054,346 + amn b1982 0.87 +0.6 2,054,346 + amn b1982 0.87 -0.4 2,054,346 + amn b1982 0.87 +1.5 2,054,347 - amn b1982 0.87 -0.5 2,054,349 - amn b1982 0.87 +0.6 2,054,351 + amn b1982 0.87 -0.6 2,054,351 + amn b1982 0.87 +0.5 2,054,351 + amn b1982 0.87 +0.6 2,054,359 - amn b1982 0.88 -1.1 2,054,463 + +1.6 2,054,473 + +0.5 2,054,476 + -0.8 2,054,514 + +1.1 2,054,514 + +0.7 2,054,581 + -0.2 2,054,581 + +1.0 2,054,612 + -0.6 2,054,620 + +0.8 2,054,893 - -0.2 2,054,893 - -1.6 2,054,963 + yeeN b1983 0.11 -2.1 2,054,968 + yeeN b1983 0.12 -2.3 2,054,985 + yeeN b1983 0.14 -1.8 2,055,129 + yeeN b1983 0.34 -1.1 2,055,130 + yeeN b1983 0.35 -1.8 2,055,139 + yeeN b1983 0.36 -1.8 2,055,146 + yeeN b1983 0.37 -1.2 2,055,250 + yeeN b1983 0.51 -0.6 2,055,262 + yeeN b1983 0.53 -0.7 2,055,262 + yeeN b1983 0.53 -1.0 2,055,264 + yeeN b1983 0.53 -1.2 2,055,264 + yeeN b1983 0.53 -3.7 2,055,264 + yeeN b1983 0.53 -1.8 2,055,267 + yeeN b1983 0.54 -0.8 2,055,277 + yeeN b1983 0.55 -1.6 2,055,309 + yeeN b1983 0.60 -2.9 2,055,309 + yeeN b1983 0.60 -1.2 2,055,354 + yeeN b1983 0.66 -0.9 2,055,354 + yeeN b1983 0.66 -1.4 2,055,354 + yeeN b1983 0.66 -1.5 2,055,364 + yeeN b1983 0.67 -0.7 2,055,364 + yeeN b1983 0.67 -1.8 2,055,364 + yeeN b1983 0.67 -1.0 2,055,412 + yeeN b1983 0.74 +0.1 2,055,420 - yeeN b1983 0.75 -2.4 2,055,430 + yeeN b1983 0.76 -2.1 2,055,430 + yeeN b1983 0.76 -2.0 2,055,443 + yeeN b1983 0.78 -0.1 2,055,443 + yeeN b1983 0.78 -1.6 2,055,454 + yeeN b1983 0.80 -1.0 2,055,487 + yeeN b1983 0.84 -1.0 2,055,502 + yeeN b1983 0.86 +0.1 2,055,522 + yeeN b1983 0.89 -1.9 2,055,543 + -1.7 2,055,543 + -3.4 2,055,543 + -1.1 2,055,782 - +1.0 2,055,852 - -0.7 2,055,857 - +0.3 2,055,857 - +0.3 2,055,877 - +0.2 2,055,887 + -0.1 2,055,889 + +0.2 2,055,895 - +0.4 2,055,895 - -0.0 2,055,895 - +1.0 2,055,897 - +2.0 2,055,930 - +0.1 2,056,031 - -0.1 2,056,031 - +1.2 2,056,032 - +0.9 2,056,075 - asnW b1984 0.32 -1.0 2,056,312 - +0.7 2,056,322 - -1.2 2,056,322 - -0.6 2,056,326 + +0.0 2,056,445 + yeeO b1985 0.15 +0.0 2,056,480 + yeeO b1985 0.17 +0.7 2,056,557 + yeeO b1985 0.22 +0.5
Or see this region's nucleotide sequence