Strain Fitness in Escherichia coli BW25113 around b1864

Experiment: Putrescine (N)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntyebB and ruvC are separated by 1 nucleotidesruvC and yebC are separated by 34 nucleotidesyebC and nudB are separated by 28 nucleotidesnudB and aspS are separated by 117 nucleotides b1862: yebB - orf, hypothetical protein (VIMSS), at 1,944,275 to 1,944,877 yebB b1863: ruvC - Holliday junction resolvase (NCBI), at 1,944,879 to 1,945,400 ruvC b1864: yebC - hypothetical protein (NCBI), at 1,945,435 to 1,946,175 yebC b1865: nudB - dATP pyrophosphohydrolase (NCBI), at 1,946,204 to 1,946,656 nudB b1866: aspS - aspartyl-tRNA synthetase (NCBI), at 1,946,774 to 1,948,546 aspS Position (kb) 1945 1946 1947Strain fitness (log2 ratio) -2 -1 0 1at 1944.511 kb on - strand, within yebBat 1944.560 kb on - strand, within yebBat 1944.582 kb on + strand, within yebBat 1944.622 kb on + strand, within yebBat 1944.625 kb on - strand, within yebBat 1944.625 kb on - strand, within yebBat 1944.625 kb on - strand, within yebBat 1944.669 kb on - strand, within yebBat 1944.674 kb on - strand, within yebBat 1944.723 kb on - strand, within yebBat 1944.818 kb on + strandat 1944.818 kb on + strandat 1944.826 kb on - strandat 1944.826 kb on - strandat 1944.829 kb on + strandat 1944.846 kb on - strandat 1944.885 kb on - strandat 1944.885 kb on - strandat 1944.895 kb on - strandat 1944.908 kb on + strandat 1944.916 kb on - strandat 1945.037 kb on - strand, within ruvCat 1945.078 kb on - strand, within ruvCat 1945.219 kb on + strand, within ruvCat 1945.255 kb on - strand, within ruvCat 1945.260 kb on - strand, within ruvCat 1945.307 kb on + strand, within ruvCat 1945.323 kb on - strand, within ruvCat 1945.396 kb on - strandat 1945.455 kb on + strandat 1945.524 kb on - strand, within yebCat 1945.557 kb on - strand, within yebCat 1945.557 kb on - strand, within yebCat 1945.623 kb on - strand, within yebCat 1945.623 kb on - strand, within yebCat 1945.640 kb on - strand, within yebCat 1945.640 kb on - strand, within yebCat 1945.652 kb on + strand, within yebCat 1945.663 kb on - strand, within yebCat 1945.726 kb on - strand, within yebCat 1945.975 kb on + strand, within yebCat 1945.975 kb on + strand, within yebCat 1945.997 kb on + strand, within yebCat 1946.123 kb on - strandat 1946.247 kb on + strandat 1946.247 kb on + strandat 1946.435 kb on + strand, within nudBat 1946.471 kb on - strand, within nudBat 1946.471 kb on - strand, within nudBat 1946.636 kb on + strandat 1946.644 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Putrescine (N)
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1,944,511 - yebB b1862 0.39 -0.4
1,944,560 - yebB b1862 0.47 +0.3
1,944,582 + yebB b1862 0.51 -0.9
1,944,622 + yebB b1862 0.58 +0.1
1,944,625 - yebB b1862 0.58 +0.7
1,944,625 - yebB b1862 0.58 +0.1
1,944,625 - yebB b1862 0.58 -1.4
1,944,669 - yebB b1862 0.65 +0.2
1,944,674 - yebB b1862 0.66 +0.1
1,944,723 - yebB b1862 0.74 -0.5
1,944,818 + +1.2
1,944,818 + +0.8
1,944,826 - -0.7
1,944,826 - -1.4
1,944,829 + +0.0
1,944,846 - -0.2
1,944,885 - +0.5
1,944,885 - -0.5
1,944,895 - +0.1
1,944,908 + -0.8
1,944,916 - +0.0
1,945,037 - ruvC b1863 0.30 -1.2
1,945,078 - ruvC b1863 0.38 +0.9
1,945,219 + ruvC b1863 0.65 -1.6
1,945,255 - ruvC b1863 0.72 -2.4
1,945,260 - ruvC b1863 0.73 -1.0
1,945,307 + ruvC b1863 0.82 -1.5
1,945,323 - ruvC b1863 0.85 -2.1
1,945,396 - -2.1
1,945,455 + -0.7
1,945,524 - yebC b1864 0.12 -1.9
1,945,557 - yebC b1864 0.16 -1.3
1,945,557 - yebC b1864 0.16 -1.6
1,945,623 - yebC b1864 0.25 -1.5
1,945,623 - yebC b1864 0.25 -2.0
1,945,640 - yebC b1864 0.28 +0.7
1,945,640 - yebC b1864 0.28 -0.6
1,945,652 + yebC b1864 0.29 -0.7
1,945,663 - yebC b1864 0.31 -0.4
1,945,726 - yebC b1864 0.39 -1.2
1,945,975 + yebC b1864 0.73 -2.0
1,945,975 + yebC b1864 0.73 -0.6
1,945,997 + yebC b1864 0.76 -1.1
1,946,123 - -2.3
1,946,247 + -2.2
1,946,247 + -2.5
1,946,435 + nudB b1865 0.51 -1.4
1,946,471 - nudB b1865 0.59 -1.9
1,946,471 - nudB b1865 0.59 -1.7
1,946,636 + -1.3
1,946,644 - +0.4

Or see this region's nucleotide sequence