Strain Fitness in Escherichia coli BW25113 around b1294

Experiment: Putrescine (N)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntsapC and sapB overlap by 14 nucleotidessapB and sapA overlap by 4 nucleotidessapA and ymjA are separated by 312 nucleotidesymjA and ycjJ are separated by 133 nucleotides b1292: sapC - predicted antimicrobial peptide transporter subunit (NCBI), at 1,351,652 to 1,352,542 sapC b1293: sapB - predicted antimicrobial peptide transporter subunit (NCBI), at 1,352,529 to 1,353,494 sapB b1294: sapA - predicted antimicrobial peptide transporter subunit (NCBI), at 1,353,491 to 1,355,134 sapA b1295: ymjA - hypothetical protein (NCBI), at 1,355,447 to 1,355,692 ymjA b1296: ycjJ - putative amino acid/amine transport protein (VIMSS), at 1,355,826 to 1,357,211 ycjJ Position (kb) 1353 1354 1355 1356Strain fitness (log2 ratio) -3 -2 -1 0 1 2at 1352.770 kb on - strand, within sapBat 1352.770 kb on - strand, within sapBat 1352.790 kb on + strand, within sapBat 1353.131 kb on + strand, within sapBat 1353.522 kb on + strandat 1353.566 kb on - strandat 1353.747 kb on + strand, within sapAat 1353.747 kb on + strand, within sapAat 1353.825 kb on + strand, within sapAat 1353.825 kb on + strand, within sapAat 1353.841 kb on + strand, within sapAat 1353.841 kb on + strand, within sapAat 1353.844 kb on - strand, within sapAat 1353.890 kb on - strand, within sapAat 1353.890 kb on - strand, within sapAat 1353.912 kb on - strand, within sapAat 1353.987 kb on + strand, within sapAat 1354.118 kb on - strand, within sapAat 1354.136 kb on + strand, within sapAat 1354.136 kb on + strand, within sapAat 1354.136 kb on + strand, within sapAat 1354.136 kb on + strand, within sapAat 1354.320 kb on - strand, within sapAat 1354.330 kb on - strand, within sapAat 1354.427 kb on - strand, within sapAat 1354.486 kb on - strand, within sapAat 1354.486 kb on - strand, within sapAat 1354.640 kb on + strand, within sapAat 1354.789 kb on - strand, within sapAat 1354.915 kb on - strand, within sapAat 1354.915 kb on - strand, within sapAat 1354.998 kb on - strandat 1355.215 kb on + strandat 1355.225 kb on - strandat 1355.225 kb on - strandat 1355.370 kb on - strandat 1355.370 kb on - strandat 1355.445 kb on - strandat 1355.485 kb on + strand, within ymjAat 1355.488 kb on - strand, within ymjAat 1355.488 kb on - strand, within ymjAat 1355.507 kb on - strand, within ymjAat 1355.713 kb on - strandat 1355.723 kb on - strandat 1355.726 kb on - strandat 1355.728 kb on - strandat 1355.853 kb on - strandat 1355.862 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Putrescine (N)
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1,352,770 - sapB b1293 0.25 +1.1
1,352,770 - sapB b1293 0.25 +2.6
1,352,790 + sapB b1293 0.27 -2.4
1,353,131 + sapB b1293 0.62 -2.1
1,353,522 + +0.8
1,353,566 - +0.8
1,353,747 + sapA b1294 0.16 +0.3
1,353,747 + sapA b1294 0.16 +1.2
1,353,825 + sapA b1294 0.20 -1.2
1,353,825 + sapA b1294 0.20 -1.0
1,353,841 + sapA b1294 0.21 -2.3
1,353,841 + sapA b1294 0.21 -0.7
1,353,844 - sapA b1294 0.21 +1.2
1,353,890 - sapA b1294 0.24 +1.1
1,353,890 - sapA b1294 0.24 +2.4
1,353,912 - sapA b1294 0.26 +1.7
1,353,987 + sapA b1294 0.30 -0.9
1,354,118 - sapA b1294 0.38 +1.5
1,354,136 + sapA b1294 0.39 -0.1
1,354,136 + sapA b1294 0.39 -0.2
1,354,136 + sapA b1294 0.39 +1.4
1,354,136 + sapA b1294 0.39 +0.4
1,354,320 - sapA b1294 0.50 -0.1
1,354,330 - sapA b1294 0.51 +0.3
1,354,427 - sapA b1294 0.57 +0.9
1,354,486 - sapA b1294 0.61 +1.5
1,354,486 - sapA b1294 0.61 +2.0
1,354,640 + sapA b1294 0.70 -1.9
1,354,789 - sapA b1294 0.79 +1.8
1,354,915 - sapA b1294 0.87 +0.3
1,354,915 - sapA b1294 0.87 +1.4
1,354,998 - -0.3
1,355,215 + -1.0
1,355,225 - +0.3
1,355,225 - +0.2
1,355,370 - +0.1
1,355,370 - +1.3
1,355,445 - -0.2
1,355,485 + ymjA b1295 0.15 -0.8
1,355,488 - ymjA b1295 0.17 +0.5
1,355,488 - ymjA b1295 0.17 +0.2
1,355,507 - ymjA b1295 0.24 -0.3
1,355,713 - -0.1
1,355,723 - +0.3
1,355,726 - -0.3
1,355,728 - -0.6
1,355,853 - -1.7
1,355,862 + -3.4

Or see this region's nucleotide sequence