Experiment: Putrescine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt trpD and trpE overlap by 1 nucleotides trpE and trpL are separated by 91 nucleotides trpL and yciV are separated by 137 nucleotides
b1263: trpD - bifunctional indole-3-glycerol-phosphate synthase/anthranilate phosphoribosyltransferase (NCBI), at 1,317,813 to 1,319,408
trpD
b1264: trpE - anthranilate synthase component I (NCBI), at 1,319,408 to 1,320,970
trpE
b1265: trpL - trp operon leader peptide (NCBI), at 1,321,062 to 1,321,106
trpL
b1266: yciV - hypothetical protein (NCBI), at 1,321,244 to 1,322,125
yciV
Position (kb)
1319
1320
1321 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1 at 1319.016 kb on - strand, within trpD at 1319.138 kb on - strand, within trpD at 1319.228 kb on + strand, within trpD at 1319.372 kb on - strand at 1319.455 kb on - strand at 1319.714 kb on + strand, within trpE at 1319.820 kb on + strand, within trpE at 1320.027 kb on + strand, within trpE at 1320.044 kb on + strand, within trpE at 1320.100 kb on - strand, within trpE at 1320.119 kb on - strand, within trpE at 1320.125 kb on + strand, within trpE at 1320.125 kb on + strand, within trpE at 1320.275 kb on - strand, within trpE at 1320.275 kb on - strand, within trpE at 1320.672 kb on - strand, within trpE at 1320.809 kb on - strand, within trpE at 1320.890 kb on - strand at 1321.053 kb on - strand at 1321.053 kb on - strand at 1321.257 kb on + strand at 1321.332 kb on + strand at 1321.332 kb on + strand at 1321.378 kb on + strand, within yciV at 1321.381 kb on + strand, within yciV at 1321.537 kb on - strand, within yciV at 1321.537 kb on - strand, within yciV at 1321.697 kb on + strand, within yciV at 1321.935 kb on - strand, within yciV
Per-strain Table
Position Strand Gene LocusTag Fraction Putrescine (N) remove 1,319,016 - trpD b1263 0.75 -2.4 1,319,138 - trpD b1263 0.83 -2.1 1,319,228 + trpD b1263 0.89 -1.3 1,319,372 - -2.5 1,319,455 - -0.1 1,319,714 + trpE b1264 0.20 -2.6 1,319,820 + trpE b1264 0.26 -4.4 1,320,027 + trpE b1264 0.40 -3.5 1,320,044 + trpE b1264 0.41 -5.6 1,320,100 - trpE b1264 0.44 -1.1 1,320,119 - trpE b1264 0.45 -1.5 1,320,125 + trpE b1264 0.46 -3.3 1,320,125 + trpE b1264 0.46 -1.6 1,320,275 - trpE b1264 0.55 +0.5 1,320,275 - trpE b1264 0.55 -0.0 1,320,672 - trpE b1264 0.81 +0.3 1,320,809 - trpE b1264 0.90 -0.5 1,320,890 - -1.3 1,321,053 - -0.4 1,321,053 - -0.1 1,321,257 + -0.2 1,321,332 + +0.9 1,321,332 + +0.4 1,321,378 + yciV b1266 0.15 -0.2 1,321,381 + yciV b1266 0.16 +0.9 1,321,537 - yciV b1266 0.33 +1.0 1,321,537 - yciV b1266 0.33 +0.2 1,321,697 + yciV b1266 0.51 -0.6 1,321,935 - yciV b1266 0.78 -0.8
Or see this region's nucleotide sequence