Experiment: Putrescine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ycgX and ycgE are separated by 220 nucleotides ycgE and ycgF are separated by 204 nucleotides
b1161: ycgX - hypothetical protein (NCBI), at 1,211,926 to 1,212,330
ycgX
b1162: ycgE - predicted DNA-binding transcriptional regulator (NCBI), at 1,212,551 to 1,213,282
ycgE
b1163: ycgF - predicted FAD-binding phosphodiesterase (NCBI), at 1,213,487 to 1,214,698
ycgF
Position (kb)
1212
1213
1214 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1212.255 kb on - strand, within ycgX at 1212.292 kb on - strand at 1212.292 kb on - strand at 1212.327 kb on - strand at 1212.328 kb on - strand at 1212.345 kb on - strand at 1212.371 kb on - strand at 1212.371 kb on - strand at 1212.376 kb on - strand at 1212.376 kb on - strand at 1212.425 kb on - strand at 1212.427 kb on + strand at 1212.435 kb on - strand at 1212.435 kb on - strand at 1212.472 kb on + strand at 1212.477 kb on + strand at 1212.477 kb on + strand at 1212.483 kb on + strand at 1212.483 kb on + strand at 1212.579 kb on - strand at 1212.579 kb on - strand at 1212.596 kb on - strand at 1212.618 kb on + strand at 1212.689 kb on - strand, within ycgE at 1212.689 kb on - strand, within ycgE at 1212.803 kb on + strand, within ycgE at 1212.864 kb on - strand, within ycgE at 1212.892 kb on - strand, within ycgE at 1212.894 kb on + strand, within ycgE at 1212.951 kb on - strand, within ycgE at 1212.954 kb on + strand, within ycgE at 1212.993 kb on + strand, within ycgE at 1213.017 kb on - strand, within ycgE at 1213.051 kb on - strand, within ycgE at 1213.051 kb on - strand, within ycgE at 1213.160 kb on - strand, within ycgE at 1213.171 kb on + strand, within ycgE at 1213.247 kb on + strand at 1213.265 kb on - strand at 1213.265 kb on - strand at 1213.277 kb on - strand at 1213.301 kb on + strand at 1213.314 kb on + strand at 1213.314 kb on + strand at 1213.314 kb on + strand at 1213.314 kb on + strand at 1213.434 kb on - strand at 1213.517 kb on - strand at 1213.581 kb on + strand at 1213.592 kb on + strand at 1213.592 kb on + strand at 1213.594 kb on + strand at 1213.600 kb on - strand at 1213.602 kb on - strand at 1213.612 kb on - strand, within ycgF at 1213.612 kb on - strand, within ycgF at 1213.620 kb on - strand, within ycgF at 1213.758 kb on - strand, within ycgF at 1213.759 kb on - strand, within ycgF at 1213.789 kb on + strand, within ycgF at 1213.789 kb on + strand, within ycgF at 1213.797 kb on - strand, within ycgF at 1213.799 kb on + strand, within ycgF at 1213.799 kb on - strand, within ycgF at 1213.846 kb on - strand, within ycgF at 1213.858 kb on - strand, within ycgF at 1213.902 kb on + strand, within ycgF at 1213.910 kb on - strand, within ycgF at 1213.960 kb on - strand, within ycgF at 1213.993 kb on - strand, within ycgF at 1213.993 kb on - strand, within ycgF at 1214.007 kb on + strand, within ycgF at 1214.067 kb on + strand, within ycgF at 1214.067 kb on + strand, within ycgF at 1214.151 kb on - strand, within ycgF at 1214.190 kb on + strand, within ycgF at 1214.190 kb on + strand, within ycgF at 1214.194 kb on - strand, within ycgF at 1214.203 kb on + strand, within ycgF at 1214.203 kb on + strand, within ycgF at 1214.203 kb on + strand, within ycgF at 1214.257 kb on + strand, within ycgF
Per-strain Table
Position Strand Gene LocusTag Fraction Putrescine (N) remove 1,212,255 - ycgX b1161 0.81 -0.3 1,212,292 - -0.1 1,212,292 - +0.6 1,212,327 - +0.3 1,212,328 - +1.1 1,212,345 - +0.9 1,212,371 - -0.2 1,212,371 - +0.0 1,212,376 - +1.1 1,212,376 - +1.0 1,212,425 - +1.0 1,212,427 + -1.0 1,212,435 - +0.1 1,212,435 - +0.5 1,212,472 + +1.1 1,212,477 + -0.8 1,212,477 + -0.4 1,212,483 + +0.3 1,212,483 + -0.1 1,212,579 - +0.5 1,212,579 - -1.5 1,212,596 - -3.5 1,212,618 + -2.4 1,212,689 - ycgE b1162 0.19 -1.0 1,212,689 - ycgE b1162 0.19 -0.4 1,212,803 + ycgE b1162 0.34 -0.8 1,212,864 - ycgE b1162 0.43 -1.3 1,212,892 - ycgE b1162 0.47 -0.1 1,212,894 + ycgE b1162 0.47 -0.2 1,212,951 - ycgE b1162 0.55 -1.4 1,212,954 + ycgE b1162 0.55 -0.8 1,212,993 + ycgE b1162 0.60 -1.5 1,213,017 - ycgE b1162 0.64 -2.6 1,213,051 - ycgE b1162 0.68 -2.6 1,213,051 - ycgE b1162 0.68 -1.7 1,213,160 - ycgE b1162 0.83 -1.2 1,213,171 + ycgE b1162 0.85 -1.0 1,213,247 + +0.3 1,213,265 - -1.5 1,213,265 - -0.5 1,213,277 - -1.9 1,213,301 + -0.9 1,213,314 + -1.4 1,213,314 + -0.1 1,213,314 + -0.6 1,213,314 + -0.6 1,213,434 - +0.1 1,213,517 - +0.2 1,213,581 + -2.7 1,213,592 + +1.4 1,213,592 + -0.1 1,213,594 + -0.4 1,213,600 - +0.0 1,213,602 - -0.6 1,213,612 - ycgF b1163 0.10 +1.8 1,213,612 - ycgF b1163 0.10 -0.1 1,213,620 - ycgF b1163 0.11 +0.4 1,213,758 - ycgF b1163 0.22 +0.7 1,213,759 - ycgF b1163 0.22 -0.5 1,213,789 + ycgF b1163 0.25 +0.6 1,213,789 + ycgF b1163 0.25 -0.1 1,213,797 - ycgF b1163 0.26 +0.3 1,213,799 + ycgF b1163 0.26 +0.1 1,213,799 - ycgF b1163 0.26 -0.1 1,213,846 - ycgF b1163 0.30 +0.2 1,213,858 - ycgF b1163 0.31 +0.3 1,213,902 + ycgF b1163 0.34 -1.3 1,213,910 - ycgF b1163 0.35 +0.1 1,213,960 - ycgF b1163 0.39 +0.1 1,213,993 - ycgF b1163 0.42 -1.2 1,213,993 - ycgF b1163 0.42 +0.6 1,214,007 + ycgF b1163 0.43 -0.5 1,214,067 + ycgF b1163 0.48 +0.5 1,214,067 + ycgF b1163 0.48 +1.0 1,214,151 - ycgF b1163 0.55 +0.4 1,214,190 + ycgF b1163 0.58 +0.7 1,214,190 + ycgF b1163 0.58 +0.3 1,214,194 - ycgF b1163 0.58 -1.3 1,214,203 + ycgF b1163 0.59 -0.2 1,214,203 + ycgF b1163 0.59 -1.8 1,214,203 + ycgF b1163 0.59 -0.2 1,214,257 + ycgF b1163 0.64 +1.4
Or see this region's nucleotide sequence