Experiment: Putrescine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt valZ and lysY are separated by 3 nucleotides lysY and lysZ are separated by 146 nucleotides lysZ and lysQ are separated by 132 nucleotides lysQ and nadA are separated by 432 nucleotides nadA and pnuC are separated by 37 nucleotides pnuC and zitB overlap by 4 nucleotides
b0746: valZ - tRNA-Val (NCBI), at 780,291 to 780,366
valZ
b0747: lysY - tRNA-Lys (NCBI), at 780,370 to 780,445
lysY
b0748: lysZ - tRNA-Lys (NCBI), at 780,592 to 780,667
lysZ
b0749: lysQ - tRNA-Lys (NCBI), at 780,800 to 780,875
lysQ
b0750: nadA - quinolinate synthetase (NCBI), at 781,308 to 782,351
nadA
b0751: pnuC - predicted nicotinamide mononucleotide transporter (NCBI), at 782,389 to 783,108
pnuC
b0752: zitB - zinc transporter ZitB (NCBI), at 783,105 to 784,046
zitB
Position (kb) 781
782
783 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1 at 780.357 kb on + strand, within valZ at 780.357 kb on + strand, within valZ at 780.536 kb on + strand at 780.536 kb on + strand at 780.583 kb on + strand at 780.703 kb on - strand at 780.705 kb on + strand at 780.708 kb on - strand at 780.729 kb on - strand at 781.165 kb on + strand at 781.257 kb on + strand at 781.257 kb on + strand at 781.262 kb on + strand at 781.262 kb on + strand at 781.319 kb on - strand at 781.319 kb on - strand at 781.430 kb on - strand, within nadA at 781.430 kb on - strand, within nadA at 781.443 kb on + strand, within nadA at 781.456 kb on - strand, within nadA at 781.456 kb on - strand, within nadA at 781.456 kb on - strand, within nadA at 781.468 kb on + strand, within nadA at 781.708 kb on + strand, within nadA at 781.760 kb on - strand, within nadA at 781.847 kb on + strand, within nadA at 781.982 kb on - strand, within nadA at 782.171 kb on - strand, within nadA at 782.171 kb on - strand, within nadA at 782.226 kb on - strand, within nadA at 782.284 kb on + strand at 782.319 kb on + strand at 782.404 kb on + strand at 782.404 kb on + strand at 782.429 kb on - strand at 782.485 kb on + strand, within pnuC at 782.508 kb on - strand, within pnuC at 782.508 kb on - strand, within pnuC at 782.510 kb on + strand, within pnuC at 782.537 kb on - strand, within pnuC at 782.701 kb on + strand, within pnuC at 782.754 kb on + strand, within pnuC at 782.803 kb on + strand, within pnuC at 782.803 kb on + strand, within pnuC at 782.824 kb on + strand, within pnuC at 782.824 kb on + strand, within pnuC at 782.848 kb on - strand, within pnuC at 782.848 kb on - strand, within pnuC at 782.888 kb on - strand, within pnuC at 782.921 kb on + strand, within pnuC at 782.921 kb on + strand, within pnuC at 783.018 kb on - strand, within pnuC at 783.127 kb on - strand at 783.158 kb on + strand at 783.179 kb on - strand at 783.193 kb on - strand at 783.193 kb on - strand at 783.202 kb on + strand, within zitB at 783.202 kb on + strand, within zitB at 783.243 kb on - strand, within zitB at 783.270 kb on + strand, within zitB at 783.270 kb on + strand, within zitB at 783.317 kb on + strand, within zitB at 783.341 kb on + strand, within zitB
Per-strain Table
Position Strand Gene LocusTag Fraction Putrescine (N) remove 780,357 + valZ b0746 0.87 -0.6 780,357 + valZ b0746 0.87 +0.7 780,536 + -0.1 780,536 + +0.5 780,583 + +0.2 780,703 - +0.3 780,705 + +0.4 780,708 - -0.1 780,729 - -0.7 781,165 + +1.2 781,257 + -0.3 781,257 + +0.5 781,262 + +0.3 781,262 + -1.2 781,319 - -3.1 781,319 - -3.1 781,430 - nadA b0750 0.12 -3.7 781,430 - nadA b0750 0.12 -2.8 781,443 + nadA b0750 0.13 -0.8 781,456 - nadA b0750 0.14 -1.9 781,456 - nadA b0750 0.14 -1.7 781,456 - nadA b0750 0.14 -2.2 781,468 + nadA b0750 0.15 -3.5 781,708 + nadA b0750 0.38 -4.3 781,760 - nadA b0750 0.43 -1.6 781,847 + nadA b0750 0.52 -1.4 781,982 - nadA b0750 0.65 -3.1 782,171 - nadA b0750 0.83 -1.4 782,171 - nadA b0750 0.83 -1.3 782,226 - nadA b0750 0.88 -1.0 782,284 + -0.8 782,319 + -1.7 782,404 + -1.1 782,404 + -1.4 782,429 - +0.7 782,485 + pnuC b0751 0.13 +1.5 782,508 - pnuC b0751 0.17 +0.1 782,508 - pnuC b0751 0.17 +0.3 782,510 + pnuC b0751 0.17 +1.2 782,537 - pnuC b0751 0.21 +0.6 782,701 + pnuC b0751 0.43 +0.2 782,754 + pnuC b0751 0.51 -0.6 782,803 + pnuC b0751 0.57 +0.2 782,803 + pnuC b0751 0.57 +0.2 782,824 + pnuC b0751 0.60 -0.1 782,824 + pnuC b0751 0.60 +0.3 782,848 - pnuC b0751 0.64 +0.6 782,848 - pnuC b0751 0.64 +0.6 782,888 - pnuC b0751 0.69 +0.3 782,921 + pnuC b0751 0.74 +0.1 782,921 + pnuC b0751 0.74 +0.7 783,018 - pnuC b0751 0.87 -0.4 783,127 - -0.2 783,158 + -0.6 783,179 - +0.7 783,193 - -1.3 783,193 - +0.3 783,202 + zitB b0752 0.10 -0.2 783,202 + zitB b0752 0.10 +0.5 783,243 - zitB b0752 0.15 -0.5 783,270 + zitB b0752 0.18 +0.2 783,270 + zitB b0752 0.18 +0.3 783,317 + zitB b0752 0.23 +0.3 783,341 + zitB b0752 0.25 -0.8
Or see this region's nucleotide sequence