Experiment: Putrescine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt miaB and ubiF are separated by 145 nucleotides ubiF and glnX are separated by 153 nucleotides glnX and glnV are separated by 37 nucleotides glnV and metU are separated by 47 nucleotides metU and glnW are separated by 15 nucleotides glnW and glnU are separated by 34 nucleotides glnU and leuW are separated by 23 nucleotides leuW and metT are separated by 9 nucleotides
b0661: miaB - isopentenyl-adenosine A37 tRNA methylthiolase (NCBI), at 692,754 to 694,178
miaB
b0662: ubiF - 2-octaprenyl-3-methyl-6-methoxy-1,4-benzoquinol hydroxylase (NCBI), at 694,324 to 695,499
ubiF
b0664: glnX - tRNA-Gln (NCBI), at 695,653 to 695,727
glnX
b0665: glnV - tRNA-Gln (NCBI), at 695,765 to 695,839
glnV
b0666: metU - tRNA-Met (NCBI), at 695,887 to 695,963
metU
b0668: glnW - tRNA-Gln (NCBI), at 695,979 to 696,053
glnW
b0670: glnU - tRNA-Gln (NCBI), at 696,088 to 696,162
glnU
b0672: leuW - tRNA-Leu (NCBI), at 696,186 to 696,270
leuW
b0673: metT - tRNA-Met (NCBI), at 696,280 to 696,356
metT
Position (kb) 694
695
696 Strain fitness (log2 ratio) -2
-1
0
1
2 at 693.337 kb on - strand, within miaB at 693.380 kb on + strand, within miaB at 693.380 kb on + strand, within miaB at 693.380 kb on + strand, within miaB at 693.595 kb on - strand, within miaB at 693.662 kb on - strand, within miaB at 693.662 kb on - strand, within miaB at 693.662 kb on - strand, within miaB at 693.662 kb on - strand, within miaB at 693.682 kb on + strand, within miaB at 693.764 kb on - strand, within miaB at 693.798 kb on + strand, within miaB at 693.798 kb on + strand, within miaB at 693.813 kb on - strand, within miaB at 694.062 kb on - strand at 694.094 kb on - strand at 694.094 kb on - strand at 694.196 kb on + strand at 694.266 kb on + strand at 695.499 kb on - strand at 695.507 kb on + strand at 695.779 kb on - strand, within glnV at 695.885 kb on - strand at 695.885 kb on - strand at 695.919 kb on - strand, within metU at 696.470 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Putrescine (N) remove 693,337 - miaB b0661 0.41 -1.2 693,380 + miaB b0661 0.44 -0.3 693,380 + miaB b0661 0.44 -1.1 693,380 + miaB b0661 0.44 -1.1 693,595 - miaB b0661 0.59 -0.8 693,662 - miaB b0661 0.64 -0.0 693,662 - miaB b0661 0.64 -1.1 693,662 - miaB b0661 0.64 -0.9 693,662 - miaB b0661 0.64 -0.4 693,682 + miaB b0661 0.65 -1.1 693,764 - miaB b0661 0.71 -0.8 693,798 + miaB b0661 0.73 -2.6 693,798 + miaB b0661 0.73 -1.9 693,813 - miaB b0661 0.74 -0.9 694,062 - -2.3 694,094 - -0.8 694,094 - -0.1 694,196 + -0.1 694,266 + -0.1 695,499 - -1.5 695,507 + +1.8 695,779 - glnV b0665 0.19 -0.2 695,885 - +0.0 695,885 - +0.9 695,919 - metU b0666 0.42 -2.2 696,470 + -0.3
Or see this region's nucleotide sequence