Experiment: Putrescine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt lacZ and lacI are separated by 122 nucleotides lacI and mhpR are separated by 76 nucleotides
b0344: lacZ - beta-D-galactosidase (NCBI), at 362,455 to 365,529
lacZ
b0345: lacI - transcriptional repressor of the lac operon (VIMSS), at 365,652 to 366,734
lacI
b0346: mhpR - DNA-binding transcriptional activator, 3HPP-binding (RefSeq), at 366,811 to 367,644
mhpR
Position (kb) 365
366
367 Strain fitness (log2 ratio) -2
-1
0
1
2 at 365.812 kb on + strand, within lacI at 365.893 kb on - strand, within lacI at 365.913 kb on + strand, within lacI at 365.919 kb on + strand, within lacI at 365.959 kb on - strand, within lacI at 365.968 kb on - strand, within lacI at 365.984 kb on + strand, within lacI at 365.987 kb on - strand, within lacI at 365.987 kb on - strand, within lacI at 366.016 kb on + strand, within lacI at 366.040 kb on - strand, within lacI at 366.040 kb on - strand, within lacI at 366.265 kb on - strand, within lacI at 366.279 kb on + strand, within lacI at 366.339 kb on - strand, within lacI at 366.339 kb on - strand, within lacI at 366.369 kb on - strand, within lacI at 366.412 kb on + strand, within lacI at 366.415 kb on - strand, within lacI at 366.455 kb on + strand, within lacI at 366.683 kb on + strand at 366.683 kb on + strand at 366.704 kb on - strand at 366.709 kb on - strand at 366.714 kb on + strand at 366.717 kb on - strand at 366.721 kb on - strand at 366.727 kb on - strand at 366.747 kb on + strand at 366.790 kb on + strand at 366.960 kb on + strand, within mhpR at 366.960 kb on + strand, within mhpR at 366.960 kb on + strand, within mhpR at 366.996 kb on + strand, within mhpR at 367.173 kb on - strand, within mhpR at 367.287 kb on + strand, within mhpR at 367.325 kb on + strand, within mhpR at 367.326 kb on - strand, within mhpR at 367.592 kb on - strand at 367.592 kb on - strand at 367.596 kb on + strand at 367.728 kb on + strand at 367.728 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Putrescine (N) remove 365,812 + lacI b0345 0.15 -0.9 365,893 - lacI b0345 0.22 -0.3 365,913 + lacI b0345 0.24 +0.7 365,919 + lacI b0345 0.25 +0.3 365,959 - lacI b0345 0.28 +0.7 365,968 - lacI b0345 0.29 +1.3 365,984 + lacI b0345 0.31 +0.3 365,987 - lacI b0345 0.31 -0.1 365,987 - lacI b0345 0.31 +0.6 366,016 + lacI b0345 0.34 +0.4 366,040 - lacI b0345 0.36 +0.2 366,040 - lacI b0345 0.36 -0.3 366,265 - lacI b0345 0.57 +0.2 366,279 + lacI b0345 0.58 -0.3 366,339 - lacI b0345 0.63 -0.4 366,339 - lacI b0345 0.63 -0.0 366,369 - lacI b0345 0.66 +0.3 366,412 + lacI b0345 0.70 -0.5 366,415 - lacI b0345 0.70 -0.4 366,455 + lacI b0345 0.74 -0.9 366,683 + -0.3 366,683 + +0.2 366,704 - +0.2 366,709 - -0.5 366,714 + -1.9 366,717 - -0.3 366,721 - +0.3 366,727 - +0.4 366,747 + -1.0 366,790 + +0.2 366,960 + mhpR b0346 0.18 +0.3 366,960 + mhpR b0346 0.18 +1.9 366,960 + mhpR b0346 0.18 -0.1 366,996 + mhpR b0346 0.22 +0.2 367,173 - mhpR b0346 0.43 -0.0 367,287 + mhpR b0346 0.57 -0.5 367,325 + mhpR b0346 0.62 +0.7 367,326 - mhpR b0346 0.62 +0.4 367,592 - -0.2 367,592 - +0.3 367,596 + -0.3 367,728 + +0.1 367,728 + +0.3
Or see this region's nucleotide sequence