Experiment: casaminos (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt yhfN and frlC are separated by 49 nucleotides frlC and frlD overlap by 4 nucleotides frlD and yhfR are separated by 99 nucleotides
b3371: yhfN - putative transport protein (VIMSS), at 3,499,290 to 3,500,312
yhfN
b4474: frlC - predicted isomerase (NCBI), at 3,500,362 to 3,501,192
frlC
b3374: frlD - fructoselysine 6-kinase (NCBI), at 3,501,189 to 3,501,974
frlD
b3375: yhfR - putative transcriptional regulator (VIMSS), at 3,502,074 to 3,502,805
yhfR
Position (kb)
3500
3501
3502 Strain fitness (log2 ratio)
-1
0
1
2 at 3499.395 kb on + strand, within yhfN at 3499.398 kb on + strand, within yhfN at 3499.398 kb on + strand, within yhfN at 3499.401 kb on - strand, within yhfN at 3499.462 kb on - strand, within yhfN at 3499.508 kb on + strand, within yhfN at 3499.508 kb on + strand, within yhfN at 3499.532 kb on + strand, within yhfN at 3499.645 kb on - strand, within yhfN at 3499.664 kb on + strand, within yhfN at 3499.675 kb on + strand, within yhfN at 3499.762 kb on - strand, within yhfN at 3499.762 kb on - strand, within yhfN at 3499.767 kb on + strand, within yhfN at 3499.767 kb on + strand, within yhfN at 3499.775 kb on - strand, within yhfN at 3499.899 kb on - strand, within yhfN at 3499.914 kb on - strand, within yhfN at 3499.929 kb on - strand, within yhfN at 3499.935 kb on - strand, within yhfN at 3499.950 kb on + strand, within yhfN at 3499.982 kb on + strand, within yhfN at 3500.052 kb on + strand, within yhfN at 3500.136 kb on - strand, within yhfN at 3500.192 kb on + strand, within yhfN at 3500.237 kb on - strand at 3500.237 kb on - strand at 3500.239 kb on + strand at 3500.245 kb on - strand at 3500.318 kb on + strand at 3500.318 kb on + strand at 3500.364 kb on + strand at 3500.400 kb on + strand at 3500.437 kb on + strand at 3500.445 kb on - strand, within frlC at 3500.476 kb on - strand, within frlC at 3500.548 kb on + strand, within frlC at 3500.548 kb on + strand, within frlC at 3500.569 kb on + strand, within frlC at 3500.588 kb on + strand, within frlC at 3500.591 kb on - strand, within frlC at 3500.595 kb on - strand, within frlC at 3500.605 kb on + strand, within frlC at 3500.605 kb on + strand, within frlC at 3500.625 kb on - strand, within frlC at 3500.625 kb on - strand, within frlC at 3500.649 kb on + strand, within frlC at 3500.680 kb on - strand, within frlC at 3500.711 kb on - strand, within frlC at 3500.718 kb on + strand, within frlC at 3500.718 kb on + strand, within frlC at 3500.726 kb on - strand, within frlC at 3500.739 kb on + strand, within frlC at 3500.763 kb on + strand, within frlC at 3500.763 kb on + strand, within frlC at 3500.763 kb on + strand, within frlC at 3500.763 kb on + strand, within frlC at 3500.767 kb on - strand, within frlC at 3500.789 kb on + strand, within frlC at 3500.815 kb on - strand, within frlC at 3500.830 kb on + strand, within frlC at 3500.833 kb on + strand, within frlC at 3500.861 kb on + strand, within frlC at 3500.861 kb on - strand, within frlC at 3500.861 kb on - strand, within frlC at 3500.899 kb on + strand, within frlC at 3501.008 kb on - strand, within frlC at 3501.020 kb on - strand, within frlC at 3501.028 kb on - strand, within frlC at 3501.035 kb on + strand, within frlC at 3501.035 kb on + strand, within frlC at 3501.062 kb on + strand, within frlC at 3501.062 kb on + strand, within frlC at 3501.090 kb on + strand, within frlC at 3501.098 kb on - strand, within frlC at 3501.100 kb on - strand, within frlC at 3501.105 kb on + strand, within frlC at 3501.114 kb on + strand at 3501.114 kb on + strand at 3501.114 kb on + strand at 3501.123 kb on - strand at 3501.123 kb on - strand at 3501.125 kb on + strand at 3501.169 kb on + strand at 3501.169 kb on + strand at 3501.178 kb on + strand at 3501.239 kb on + strand at 3501.240 kb on - strand at 3501.284 kb on + strand, within frlD at 3501.292 kb on - strand, within frlD at 3501.330 kb on + strand, within frlD at 3501.382 kb on - strand, within frlD at 3501.410 kb on + strand, within frlD at 3501.498 kb on - strand, within frlD at 3501.498 kb on - strand, within frlD at 3501.517 kb on - strand, within frlD at 3501.522 kb on - strand, within frlD at 3501.530 kb on + strand, within frlD at 3501.530 kb on + strand, within frlD at 3501.530 kb on + strand, within frlD at 3501.530 kb on + strand, within frlD at 3501.530 kb on + strand, within frlD at 3501.536 kb on - strand, within frlD at 3501.536 kb on - strand, within frlD at 3501.536 kb on - strand, within frlD at 3501.538 kb on - strand, within frlD at 3501.548 kb on - strand, within frlD at 3501.560 kb on - strand, within frlD at 3501.801 kb on + strand, within frlD at 3501.803 kb on + strand, within frlD at 3501.962 kb on - strand at 3501.964 kb on - strand at 3501.975 kb on + strand at 3501.975 kb on + strand at 3501.980 kb on + strand at 3501.998 kb on - strand at 3501.998 kb on - strand at 3502.109 kb on + strand at 3502.115 kb on - strand at 3502.147 kb on + strand at 3502.157 kb on + strand, within yhfR at 3502.161 kb on - strand, within yhfR at 3502.175 kb on - strand, within yhfR
Per-strain Table
Position Strand Gene LocusTag Fraction casaminos (N) remove 3,499,395 + yhfN b3371 0.10 -0.0 3,499,398 + yhfN b3371 0.11 -0.4 3,499,398 + yhfN b3371 0.11 +0.6 3,499,401 - yhfN b3371 0.11 +0.4 3,499,462 - yhfN b3371 0.17 +0.1 3,499,508 + yhfN b3371 0.21 +1.2 3,499,508 + yhfN b3371 0.21 +1.2 3,499,532 + yhfN b3371 0.24 -0.1 3,499,645 - yhfN b3371 0.35 +0.2 3,499,664 + yhfN b3371 0.37 -0.3 3,499,675 + yhfN b3371 0.38 +0.8 3,499,762 - yhfN b3371 0.46 +0.2 3,499,762 - yhfN b3371 0.46 +1.9 3,499,767 + yhfN b3371 0.47 -1.2 3,499,767 + yhfN b3371 0.47 -0.8 3,499,775 - yhfN b3371 0.47 +0.8 3,499,899 - yhfN b3371 0.60 +0.1 3,499,914 - yhfN b3371 0.61 +0.4 3,499,929 - yhfN b3371 0.62 -0.1 3,499,935 - yhfN b3371 0.63 -0.1 3,499,950 + yhfN b3371 0.65 -0.9 3,499,982 + yhfN b3371 0.68 +0.5 3,500,052 + yhfN b3371 0.74 -0.9 3,500,136 - yhfN b3371 0.83 -0.4 3,500,192 + yhfN b3371 0.88 -0.0 3,500,237 - +0.6 3,500,237 - +0.3 3,500,239 + -0.7 3,500,245 - -0.7 3,500,318 + +1.3 3,500,318 + +0.2 3,500,364 + -0.6 3,500,400 + -0.6 3,500,437 + +0.9 3,500,445 - frlC b4474 0.10 +0.3 3,500,476 - frlC b4474 0.14 +0.3 3,500,548 + frlC b4474 0.22 -0.6 3,500,548 + frlC b4474 0.22 +0.1 3,500,569 + frlC b4474 0.25 -0.6 3,500,588 + frlC b4474 0.27 +0.9 3,500,591 - frlC b4474 0.28 +0.1 3,500,595 - frlC b4474 0.28 +0.3 3,500,605 + frlC b4474 0.29 +0.2 3,500,605 + frlC b4474 0.29 +0.8 3,500,625 - frlC b4474 0.32 +0.3 3,500,625 - frlC b4474 0.32 -0.2 3,500,649 + frlC b4474 0.35 -0.5 3,500,680 - frlC b4474 0.38 -0.4 3,500,711 - frlC b4474 0.42 -0.0 3,500,718 + frlC b4474 0.43 -1.2 3,500,718 + frlC b4474 0.43 -0.4 3,500,726 - frlC b4474 0.44 +0.4 3,500,739 + frlC b4474 0.45 +0.4 3,500,763 + frlC b4474 0.48 +0.3 3,500,763 + frlC b4474 0.48 +0.2 3,500,763 + frlC b4474 0.48 -0.4 3,500,763 + frlC b4474 0.48 +0.1 3,500,767 - frlC b4474 0.49 -0.6 3,500,789 + frlC b4474 0.51 +0.3 3,500,815 - frlC b4474 0.55 +1.3 3,500,830 + frlC b4474 0.56 +0.1 3,500,833 + frlC b4474 0.57 -0.7 3,500,861 + frlC b4474 0.60 -0.2 3,500,861 - frlC b4474 0.60 -0.2 3,500,861 - frlC b4474 0.60 -0.2 3,500,899 + frlC b4474 0.65 +0.1 3,501,008 - frlC b4474 0.78 +0.0 3,501,020 - frlC b4474 0.79 +0.3 3,501,028 - frlC b4474 0.80 -0.3 3,501,035 + frlC b4474 0.81 -0.1 3,501,035 + frlC b4474 0.81 +0.3 3,501,062 + frlC b4474 0.84 +0.6 3,501,062 + frlC b4474 0.84 +1.3 3,501,090 + frlC b4474 0.88 -0.4 3,501,098 - frlC b4474 0.89 -0.4 3,501,100 - frlC b4474 0.89 -0.2 3,501,105 + frlC b4474 0.89 -0.4 3,501,114 + +0.2 3,501,114 + +0.3 3,501,114 + -0.6 3,501,123 - +0.5 3,501,123 - +1.1 3,501,125 + -0.3 3,501,169 + +0.2 3,501,169 + +0.0 3,501,178 + +1.2 3,501,239 + -0.2 3,501,240 - +0.2 3,501,284 + frlD b3374 0.12 +0.1 3,501,292 - frlD b3374 0.13 -0.4 3,501,330 + frlD b3374 0.18 +0.1 3,501,382 - frlD b3374 0.25 +0.1 3,501,410 + frlD b3374 0.28 +0.2 3,501,498 - frlD b3374 0.39 -1.2 3,501,498 - frlD b3374 0.39 -0.7 3,501,517 - frlD b3374 0.42 +0.2 3,501,522 - frlD b3374 0.42 +0.9 3,501,530 + frlD b3374 0.43 -0.8 3,501,530 + frlD b3374 0.43 -0.5 3,501,530 + frlD b3374 0.43 -0.0 3,501,530 + frlD b3374 0.43 -0.6 3,501,530 + frlD b3374 0.43 +0.6 3,501,536 - frlD b3374 0.44 +0.2 3,501,536 - frlD b3374 0.44 +0.2 3,501,536 - frlD b3374 0.44 -0.1 3,501,538 - frlD b3374 0.44 -0.3 3,501,548 - frlD b3374 0.46 +0.3 3,501,560 - frlD b3374 0.47 -0.5 3,501,801 + frlD b3374 0.78 +0.6 3,501,803 + frlD b3374 0.78 +0.7 3,501,962 - -0.7 3,501,964 - +0.1 3,501,975 + +0.0 3,501,975 + +0.6 3,501,980 + +0.3 3,501,998 - -0.5 3,501,998 - +0.0 3,502,109 + -1.3 3,502,115 - +0.9 3,502,147 + +0.3 3,502,157 + yhfR b3375 0.11 -0.5 3,502,161 - yhfR b3375 0.12 -1.1 3,502,175 - yhfR b3375 0.14 +0.0
Or see this region's nucleotide sequence