Strain Fitness in Escherichia coli BW25113 around b0775

Experiment: casaminos (N)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntbioA and bioB are separated by 86 nucleotidesbioB and bioF overlap by 4 nucleotides b0774: bioA - adenosylmethionine--8-amino-7-oxononanoate transaminase (NCBI), at 807,191 to 808,480 bioA b0775: bioB - biotin synthase (NCBI), at 808,567 to 809,607 bioB b0776: bioF - 8-amino-7-oxononanoate synthase (NCBI), at 809,604 to 810,758 bioF Position (kb) 808 809 810Strain fitness (log2 ratio) -3 -2 -1 0 1at 807.591 kb on + strand, within bioAat 807.597 kb on - strand, within bioAat 807.607 kb on - strand, within bioAat 807.720 kb on + strand, within bioAat 807.720 kb on + strand, within bioAat 807.848 kb on - strand, within bioAat 807.982 kb on - strand, within bioAat 807.995 kb on + strand, within bioAat 808.019 kb on - strand, within bioAat 808.050 kb on - strand, within bioAat 808.307 kb on + strand, within bioAat 808.337 kb on + strand, within bioAat 808.401 kb on - strandat 808.428 kb on + strandat 808.428 kb on + strandat 808.684 kb on + strand, within bioBat 808.904 kb on - strand, within bioBat 808.915 kb on + strand, within bioBat 808.949 kb on + strand, within bioBat 808.964 kb on + strand, within bioBat 809.074 kb on + strand, within bioBat 809.082 kb on - strand, within bioBat 809.217 kb on - strand, within bioBat 809.217 kb on - strand, within bioBat 809.233 kb on + strand, within bioBat 809.379 kb on + strand, within bioBat 809.723 kb on + strand, within bioFat 809.993 kb on + strand, within bioFat 809.993 kb on + strand, within bioFat 810.237 kb on + strand, within bioFat 810.237 kb on + strand, within bioFat 810.359 kb on + strand, within bioFat 810.362 kb on - strand, within bioFat 810.393 kb on - strand, within bioFat 810.414 kb on + strand, within bioFat 810.492 kb on + strand, within bioFat 810.492 kb on + strand, within bioF

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Per-strain Table

Position Strand Gene LocusTag Fraction casaminos (N)
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807,591 + bioA b0774 0.31 -0.3
807,597 - bioA b0774 0.31 -1.2
807,607 - bioA b0774 0.32 -1.8
807,720 + bioA b0774 0.41 -0.1
807,720 + bioA b0774 0.41 -0.6
807,848 - bioA b0774 0.51 -0.0
807,982 - bioA b0774 0.61 -1.3
807,995 + bioA b0774 0.62 -0.1
808,019 - bioA b0774 0.64 -0.2
808,050 - bioA b0774 0.67 -3.5
808,307 + bioA b0774 0.87 -1.6
808,337 + bioA b0774 0.89 -0.4
808,401 - -1.8
808,428 + -0.6
808,428 + -1.7
808,684 + bioB b0775 0.11 -1.9
808,904 - bioB b0775 0.32 -0.9
808,915 + bioB b0775 0.33 -1.3
808,949 + bioB b0775 0.37 -3.1
808,964 + bioB b0775 0.38 -1.0
809,074 + bioB b0775 0.49 -1.4
809,082 - bioB b0775 0.49 -0.9
809,217 - bioB b0775 0.62 -1.1
809,217 - bioB b0775 0.62 -1.2
809,233 + bioB b0775 0.64 -0.4
809,379 + bioB b0775 0.78 -2.4
809,723 + bioF b0776 0.10 -1.1
809,993 + bioF b0776 0.34 +0.8
809,993 + bioF b0776 0.34 -0.4
810,237 + bioF b0776 0.55 -1.2
810,237 + bioF b0776 0.55 -0.8
810,359 + bioF b0776 0.65 -1.1
810,362 - bioF b0776 0.66 -1.0
810,393 - bioF b0776 0.68 -0.6
810,414 + bioF b0776 0.70 -1.2
810,492 + bioF b0776 0.77 -1.7
810,492 + bioF b0776 0.77 +0.6

Or see this region's nucleotide sequence