Experiment: Gly-Glu (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt yobD and b1821 are separated by 428 nucleotides b1821 and rrmA overlap by 4 nucleotides rrmA and cspC are separated by 165 nucleotides cspC and yobF are separated by 12 nucleotides
b1820: yobD - hypothetical protein (NCBI), at 1,902,825 to 1,903,283
yobD
b1821: b1821 - orf, hypothetical protein (VIMSS), at 1,903,712 to 1,904,278
b1821
b1822: rrmA - 23S rRNA m1G745 methyltransferase (NCBI), at 1,904,275 to 1,905,084
rrmA
b1823: cspC - stress protein, member of the CspA-family (NCBI), at 1,905,250 to 1,905,459
cspC
b1824: yobF - hypothetical protein (NCBI), at 1,905,472 to 1,905,615
yobF
Position (kb)
1904
1905
1906 Strain fitness (log2 ratio)
-2
-1
0
1
2
3
4
5
6 at 1903.496 kb on - strand at 1903.498 kb on - strand at 1903.529 kb on + strand at 1903.537 kb on - strand at 1903.548 kb on + strand at 1903.553 kb on + strand at 1903.558 kb on + strand at 1903.558 kb on + strand at 1903.583 kb on + strand at 1903.602 kb on - strand at 1903.602 kb on - strand at 1903.639 kb on - strand at 1903.654 kb on - strand at 1903.702 kb on + strand at 1903.777 kb on - strand, within b1821 at 1903.777 kb on - strand, within b1821 at 1904.065 kb on - strand, within b1821 at 1904.087 kb on - strand, within b1821 at 1904.603 kb on + strand, within rrmA at 1904.603 kb on + strand, within rrmA at 1904.691 kb on + strand, within rrmA at 1904.897 kb on - strand, within rrmA at 1904.988 kb on - strand, within rrmA at 1905.238 kb on - strand at 1905.238 kb on - strand at 1905.348 kb on + strand, within cspC at 1905.364 kb on - strand, within cspC at 1905.432 kb on + strand, within cspC at 1905.432 kb on + strand, within cspC at 1905.534 kb on - strand, within yobF at 1905.534 kb on - strand, within yobF at 1905.536 kb on + strand, within yobF at 1905.562 kb on + strand, within yobF at 1905.562 kb on + strand, within yobF at 1905.613 kb on + strand at 1905.619 kb on - strand at 1905.629 kb on - strand at 1905.647 kb on + strand at 1905.751 kb on - strand at 1905.751 kb on - strand at 1905.771 kb on + strand at 1905.782 kb on + strand at 1905.879 kb on - strand at 1905.881 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Gly-Glu (N) remove 1,903,496 - -0.1 1,903,498 - -0.1 1,903,529 + +0.5 1,903,537 - -0.1 1,903,548 + +0.1 1,903,553 + +0.3 1,903,558 + +0.3 1,903,558 + -0.3 1,903,583 + +0.1 1,903,602 - -1.2 1,903,602 - +0.4 1,903,639 - -0.1 1,903,654 - +0.1 1,903,702 + +0.4 1,903,777 - b1821 b1821 0.11 -0.1 1,903,777 - b1821 b1821 0.11 +0.6 1,904,065 - b1821 b1821 0.62 +0.6 1,904,087 - b1821 b1821 0.66 +0.1 1,904,603 + rrmA b1822 0.40 +0.3 1,904,603 + rrmA b1822 0.40 +1.0 1,904,691 + rrmA b1822 0.51 -0.2 1,904,897 - rrmA b1822 0.77 +3.8 1,904,988 - rrmA b1822 0.88 -0.1 1,905,238 - +1.5 1,905,238 - +2.6 1,905,348 + cspC b1823 0.47 +5.5 1,905,364 - cspC b1823 0.54 +4.9 1,905,432 + cspC b1823 0.87 +5.4 1,905,432 + cspC b1823 0.87 +5.8 1,905,534 - yobF b1824 0.43 +1.5 1,905,534 - yobF b1824 0.43 +0.7 1,905,536 + yobF b1824 0.44 +5.4 1,905,562 + yobF b1824 0.62 +5.2 1,905,562 + yobF b1824 0.62 +5.2 1,905,613 + +5.5 1,905,619 - +4.6 1,905,629 - +4.5 1,905,647 + +5.6 1,905,751 - -1.6 1,905,751 - +0.1 1,905,771 + +1.0 1,905,782 + +0.9 1,905,879 - -0.6 1,905,881 - -2.0
Or see this region's nucleotide sequence