Experiment: NAG (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ABIE41_RS09675 and ABIE41_RS09680 are separated by 245 nucleotides ABIE41_RS09680 and ABIE41_RS09685 overlap by 26 nucleotides ABIE41_RS09685 and ABIE41_RS09690 are separated by 2 nucleotides
ABIE41_RS09675: ABIE41_RS09675 - malate synthase A, at 1,999,100 to 2,000,701
_RS09675
ABIE41_RS09680: ABIE41_RS09680 - pilus assembly protein, at 2,000,947 to 2,002,218
_RS09680
ABIE41_RS09685: ABIE41_RS09685 - HD family hydrolase, at 2,002,193 to 2,002,807
_RS09685
ABIE41_RS09690: ABIE41_RS09690 - DNA-3-methyladenine glycosylase I, at 2,002,810 to 2,003,454
_RS09690
Position (kb)
2000
2001
2002
2003 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 2000.062 kb on - strand, within ABIE41_RS09675 at 2000.062 kb on - strand, within ABIE41_RS09675 at 2000.067 kb on + strand, within ABIE41_RS09675 at 2000.067 kb on + strand, within ABIE41_RS09675 at 2000.067 kb on + strand, within ABIE41_RS09675 at 2000.067 kb on + strand, within ABIE41_RS09675 at 2000.068 kb on - strand, within ABIE41_RS09675 at 2000.068 kb on - strand, within ABIE41_RS09675 at 2000.068 kb on - strand, within ABIE41_RS09675 at 2000.068 kb on - strand, within ABIE41_RS09675 at 2000.115 kb on + strand, within ABIE41_RS09675 at 2000.115 kb on + strand, within ABIE41_RS09675 at 2000.115 kb on + strand, within ABIE41_RS09675 at 2000.115 kb on + strand, within ABIE41_RS09675 at 2000.116 kb on - strand, within ABIE41_RS09675 at 2000.116 kb on - strand, within ABIE41_RS09675 at 2000.235 kb on + strand, within ABIE41_RS09675 at 2000.235 kb on + strand, within ABIE41_RS09675 at 2000.235 kb on + strand, within ABIE41_RS09675 at 2000.235 kb on + strand, within ABIE41_RS09675 at 2000.236 kb on - strand, within ABIE41_RS09675 at 2000.637 kb on + strand at 2000.637 kb on + strand at 2000.838 kb on - strand at 2000.878 kb on + strand at 2001.071 kb on - strand at 2001.580 kb on + strand, within ABIE41_RS09680 at 2001.580 kb on + strand, within ABIE41_RS09680 at 2001.580 kb on + strand, within ABIE41_RS09680 at 2001.581 kb on - strand, within ABIE41_RS09680 at 2001.581 kb on - strand, within ABIE41_RS09680 at 2001.728 kb on - strand, within ABIE41_RS09680 at 2001.728 kb on - strand, within ABIE41_RS09680 at 2001.739 kb on + strand, within ABIE41_RS09680 at 2001.740 kb on - strand, within ABIE41_RS09680 at 2001.946 kb on + strand, within ABIE41_RS09680 at 2001.946 kb on + strand, within ABIE41_RS09680 at 2001.947 kb on - strand, within ABIE41_RS09680 at 2001.947 kb on - strand, within ABIE41_RS09680 at 2001.947 kb on - strand, within ABIE41_RS09680 at 2001.947 kb on - strand, within ABIE41_RS09680 at 2001.947 kb on - strand, within ABIE41_RS09680 at 2002.066 kb on + strand, within ABIE41_RS09680 at 2002.066 kb on + strand, within ABIE41_RS09680 at 2002.066 kb on + strand, within ABIE41_RS09680 at 2002.067 kb on - strand, within ABIE41_RS09680 at 2002.067 kb on - strand, within ABIE41_RS09680 at 2002.067 kb on - strand, within ABIE41_RS09680 at 2002.067 kb on - strand, within ABIE41_RS09680 at 2002.067 kb on - strand, within ABIE41_RS09680 at 2002.067 kb on - strand, within ABIE41_RS09680 at 2002.068 kb on + strand, within ABIE41_RS09680 at 2002.068 kb on + strand, within ABIE41_RS09680 at 2002.068 kb on + strand, within ABIE41_RS09680 at 2002.069 kb on - strand, within ABIE41_RS09680 at 2002.069 kb on - strand, within ABIE41_RS09680 at 2002.069 kb on - strand, within ABIE41_RS09680 at 2002.132 kb on + strand at 2002.132 kb on + strand at 2002.132 kb on + strand at 2002.132 kb on + strand at 2002.132 kb on + strand at 2002.132 kb on + strand at 2002.132 kb on + strand at 2002.133 kb on - strand at 2002.133 kb on - strand at 2002.133 kb on - strand at 2002.133 kb on - strand at 2002.133 kb on - strand at 2002.133 kb on - strand at 2002.133 kb on - strand at 2002.135 kb on + strand at 2002.135 kb on + strand at 2002.135 kb on + strand at 2002.135 kb on + strand at 2002.135 kb on + strand at 2002.135 kb on + strand at 2002.135 kb on + strand at 2002.136 kb on - strand at 2002.136 kb on - strand at 2002.136 kb on - strand at 2002.226 kb on - strand at 2002.320 kb on + strand, within ABIE41_RS09685 at 2002.321 kb on - strand, within ABIE41_RS09685 at 2002.412 kb on + strand, within ABIE41_RS09685 at 2002.412 kb on + strand, within ABIE41_RS09685 at 2002.412 kb on + strand, within ABIE41_RS09685 at 2002.412 kb on + strand, within ABIE41_RS09685 at 2002.412 kb on + strand, within ABIE41_RS09685 at 2002.412 kb on + strand, within ABIE41_RS09685 at 2002.413 kb on - strand, within ABIE41_RS09685 at 2002.485 kb on + strand, within ABIE41_RS09685 at 2002.485 kb on + strand, within ABIE41_RS09685 at 2002.485 kb on + strand, within ABIE41_RS09685 at 2002.537 kb on - strand, within ABIE41_RS09685 at 2002.537 kb on - strand, within ABIE41_RS09685 at 2002.537 kb on - strand, within ABIE41_RS09685 at 2002.537 kb on - strand, within ABIE41_RS09685 at 2002.537 kb on - strand, within ABIE41_RS09685 at 2002.564 kb on + strand, within ABIE41_RS09685 at 2002.565 kb on - strand, within ABIE41_RS09685 at 2002.565 kb on - strand, within ABIE41_RS09685 at 2002.913 kb on + strand, within ABIE41_RS09690 at 2002.914 kb on - strand, within ABIE41_RS09690 at 2002.914 kb on - strand, within ABIE41_RS09690 at 2002.914 kb on - strand, within ABIE41_RS09690 at 2003.093 kb on + strand, within ABIE41_RS09690 at 2003.093 kb on + strand, within ABIE41_RS09690 at 2003.094 kb on - strand, within ABIE41_RS09690 at 2003.094 kb on - strand, within ABIE41_RS09690
Per-strain Table
Position Strand Gene LocusTag Fraction NAG (C) remove 2,000,062 - ABIE41_RS09675 0.60 +1.4 2,000,062 - ABIE41_RS09675 0.60 +1.4 2,000,067 + ABIE41_RS09675 0.60 -1.7 2,000,067 + ABIE41_RS09675 0.60 -0.2 2,000,067 + ABIE41_RS09675 0.60 -2.5 2,000,067 + ABIE41_RS09675 0.60 -0.4 2,000,068 - ABIE41_RS09675 0.60 -0.1 2,000,068 - ABIE41_RS09675 0.60 +1.2 2,000,068 - ABIE41_RS09675 0.60 +0.4 2,000,068 - ABIE41_RS09675 0.60 +1.7 2,000,115 + ABIE41_RS09675 0.63 -0.7 2,000,115 + ABIE41_RS09675 0.63 -2.0 2,000,115 + ABIE41_RS09675 0.63 +0.1 2,000,115 + ABIE41_RS09675 0.63 -0.4 2,000,116 - ABIE41_RS09675 0.63 -0.5 2,000,116 - ABIE41_RS09675 0.63 +2.7 2,000,235 + ABIE41_RS09675 0.71 -0.4 2,000,235 + ABIE41_RS09675 0.71 -0.9 2,000,235 + ABIE41_RS09675 0.71 +0.2 2,000,235 + ABIE41_RS09675 0.71 -1.5 2,000,236 - ABIE41_RS09675 0.71 -1.1 2,000,637 + -1.2 2,000,637 + +0.5 2,000,838 - +0.2 2,000,878 + +0.8 2,001,071 - +0.2 2,001,580 + ABIE41_RS09680 0.50 -0.5 2,001,580 + ABIE41_RS09680 0.50 -0.7 2,001,580 + ABIE41_RS09680 0.50 +0.3 2,001,581 - ABIE41_RS09680 0.50 -2.0 2,001,581 - ABIE41_RS09680 0.50 +1.6 2,001,728 - ABIE41_RS09680 0.61 -0.4 2,001,728 - ABIE41_RS09680 0.61 +1.2 2,001,739 + ABIE41_RS09680 0.62 -0.0 2,001,740 - ABIE41_RS09680 0.62 -4.5 2,001,946 + ABIE41_RS09680 0.79 -1.5 2,001,946 + ABIE41_RS09680 0.79 +0.6 2,001,947 - ABIE41_RS09680 0.79 +0.3 2,001,947 - ABIE41_RS09680 0.79 +0.0 2,001,947 - ABIE41_RS09680 0.79 +0.0 2,001,947 - ABIE41_RS09680 0.79 -0.4 2,001,947 - ABIE41_RS09680 0.79 -0.2 2,002,066 + ABIE41_RS09680 0.88 +0.4 2,002,066 + ABIE41_RS09680 0.88 +0.3 2,002,066 + ABIE41_RS09680 0.88 +0.8 2,002,067 - ABIE41_RS09680 0.88 +0.3 2,002,067 - ABIE41_RS09680 0.88 -0.4 2,002,067 - ABIE41_RS09680 0.88 +0.3 2,002,067 - ABIE41_RS09680 0.88 +2.6 2,002,067 - ABIE41_RS09680 0.88 -0.8 2,002,067 - ABIE41_RS09680 0.88 +1.0 2,002,068 + ABIE41_RS09680 0.88 -0.2 2,002,068 + ABIE41_RS09680 0.88 -0.0 2,002,068 + ABIE41_RS09680 0.88 +0.2 2,002,069 - ABIE41_RS09680 0.88 +1.0 2,002,069 - ABIE41_RS09680 0.88 +0.0 2,002,069 - ABIE41_RS09680 0.88 -0.7 2,002,132 + -0.1 2,002,132 + +1.2 2,002,132 + -1.1 2,002,132 + -0.2 2,002,132 + -0.4 2,002,132 + +0.4 2,002,132 + -0.5 2,002,133 - -2.0 2,002,133 - +1.0 2,002,133 - -0.0 2,002,133 - -0.0 2,002,133 - -0.5 2,002,133 - +0.3 2,002,133 - +0.3 2,002,135 + -0.5 2,002,135 + +0.4 2,002,135 + -0.9 2,002,135 + +0.1 2,002,135 + -1.8 2,002,135 + +1.4 2,002,135 + +0.3 2,002,136 - +0.7 2,002,136 - -1.6 2,002,136 - -0.6 2,002,226 - -0.9 2,002,320 + ABIE41_RS09685 0.21 -1.1 2,002,321 - ABIE41_RS09685 0.21 +1.1 2,002,412 + ABIE41_RS09685 0.36 +0.1 2,002,412 + ABIE41_RS09685 0.36 -0.5 2,002,412 + ABIE41_RS09685 0.36 -0.3 2,002,412 + ABIE41_RS09685 0.36 -0.1 2,002,412 + ABIE41_RS09685 0.36 +0.5 2,002,412 + ABIE41_RS09685 0.36 -0.4 2,002,413 - ABIE41_RS09685 0.36 +0.4 2,002,485 + ABIE41_RS09685 0.47 -0.8 2,002,485 + ABIE41_RS09685 0.47 +1.2 2,002,485 + ABIE41_RS09685 0.47 -0.7 2,002,537 - ABIE41_RS09685 0.56 -0.9 2,002,537 - ABIE41_RS09685 0.56 -2.7 2,002,537 - ABIE41_RS09685 0.56 +0.6 2,002,537 - ABIE41_RS09685 0.56 -1.8 2,002,537 - ABIE41_RS09685 0.56 -0.5 2,002,564 + ABIE41_RS09685 0.60 -1.4 2,002,565 - ABIE41_RS09685 0.60 +0.0 2,002,565 - ABIE41_RS09685 0.60 -1.0 2,002,913 + ABIE41_RS09690 0.16 +0.3 2,002,914 - ABIE41_RS09690 0.16 +0.5 2,002,914 - ABIE41_RS09690 0.16 -1.8 2,002,914 - ABIE41_RS09690 0.16 -0.8 2,003,093 + ABIE41_RS09690 0.44 -0.6 2,003,093 + ABIE41_RS09690 0.44 +0.6 2,003,094 - ABIE41_RS09690 0.44 +0.1 2,003,094 - ABIE41_RS09690 0.44 -0.1
Or see this region's nucleotide sequence