Experiment: Thymidine (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ABIE40_RS27045 and ABIE40_RS27050 are separated by 494 nucleotides ABIE40_RS27050 and ABIE40_RS27055 are separated by 130 nucleotides ABIE40_RS27055 and ABIE40_RS27060 are separated by 348 nucleotides
ABIE40_RS27045: ABIE40_RS27045 - alpha/beta hydrolase, at 127,295 to 128,065
_RS27045
ABIE40_RS27050: ABIE40_RS27050 - hypothetical protein, at 128,560 to 128,958
_RS27050
ABIE40_RS27055: ABIE40_RS27055 - transposase, at 129,089 to 129,445
_RS27055
ABIE40_RS27060: ABIE40_RS27060 - ISNCY family transposase, at 129,794 to 131,166
_RS27060
Position (kb)
128
129 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1
2
3 at 127.560 kb on - strand, within ABIE40_RS27045 at 127.560 kb on - strand, within ABIE40_RS27045 at 127.560 kb on - strand, within ABIE40_RS27045 at 127.759 kb on + strand, within ABIE40_RS27045 at 127.820 kb on - strand, within ABIE40_RS27045 at 127.899 kb on + strand, within ABIE40_RS27045 at 127.899 kb on + strand, within ABIE40_RS27045 at 127.901 kb on + strand, within ABIE40_RS27045 at 127.901 kb on + strand, within ABIE40_RS27045 at 127.901 kb on + strand, within ABIE40_RS27045 at 127.902 kb on - strand, within ABIE40_RS27045 at 128.004 kb on + strand at 128.005 kb on - strand at 128.005 kb on - strand at 128.029 kb on - strand at 128.059 kb on + strand at 128.060 kb on - strand at 128.071 kb on - strand at 128.172 kb on + strand at 128.172 kb on + strand at 128.172 kb on + strand at 128.173 kb on - strand at 128.174 kb on + strand at 128.175 kb on - strand at 128.175 kb on - strand at 128.217 kb on + strand at 128.431 kb on - strand at 128.449 kb on + strand at 128.450 kb on - strand at 128.450 kb on - strand at 128.450 kb on - strand at 128.572 kb on + strand at 128.598 kb on - strand at 128.598 kb on - strand at 128.598 kb on - strand at 128.629 kb on - strand, within ABIE40_RS27050 at 128.629 kb on - strand, within ABIE40_RS27050 at 128.719 kb on + strand, within ABIE40_RS27050 at 128.720 kb on - strand, within ABIE40_RS27050 at 128.732 kb on + strand, within ABIE40_RS27050 at 128.732 kb on + strand, within ABIE40_RS27050 at 128.733 kb on - strand, within ABIE40_RS27050 at 128.733 kb on - strand, within ABIE40_RS27050 at 128.733 kb on - strand, within ABIE40_RS27050 at 128.933 kb on + strand at 128.934 kb on - strand at 128.934 kb on - strand at 129.322 kb on + strand, within ABIE40_RS27055 at 129.322 kb on + strand, within ABIE40_RS27055 at 129.323 kb on - strand, within ABIE40_RS27055 at 129.392 kb on + strand, within ABIE40_RS27055 at 129.393 kb on - strand, within ABIE40_RS27055 at 129.393 kb on - strand, within ABIE40_RS27055 at 129.393 kb on - strand, within ABIE40_RS27055 at 129.393 kb on - strand, within ABIE40_RS27055 at 129.393 kb on - strand, within ABIE40_RS27055 at 129.432 kb on + strand at 129.432 kb on + strand at 129.514 kb on + strand at 129.515 kb on - strand at 129.515 kb on - strand at 129.631 kb on - strand at 129.637 kb on + strand at 129.637 kb on + strand at 129.637 kb on + strand at 129.638 kb on - strand at 129.638 kb on - strand at 129.639 kb on + strand at 129.639 kb on + strand at 129.640 kb on - strand at 129.640 kb on - strand at 129.640 kb on - strand at 129.640 kb on - strand at 129.644 kb on + strand at 129.645 kb on - strand at 129.646 kb on + strand at 129.690 kb on - strand at 129.870 kb on + strand at 129.870 kb on + strand at 129.870 kb on + strand at 129.870 kb on + strand at 129.870 kb on + strand at 129.871 kb on - strand at 129.871 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Thymidine (C) remove 127,560 - ABIE40_RS27045 0.34 +0.3 127,560 - ABIE40_RS27045 0.34 -2.4 127,560 - ABIE40_RS27045 0.34 -3.3 127,759 + ABIE40_RS27045 0.60 +0.4 127,820 - ABIE40_RS27045 0.68 -2.6 127,899 + ABIE40_RS27045 0.78 +1.3 127,899 + ABIE40_RS27045 0.78 +1.5 127,901 + ABIE40_RS27045 0.79 +0.9 127,901 + ABIE40_RS27045 0.79 -3.4 127,901 + ABIE40_RS27045 0.79 +0.1 127,902 - ABIE40_RS27045 0.79 +2.1 128,004 + -0.3 128,005 - -3.4 128,005 - +0.1 128,029 - +0.9 128,059 + -3.8 128,060 - -1.3 128,071 - -0.7 128,172 + -1.4 128,172 + -0.2 128,172 + -1.9 128,173 - -1.2 128,174 + +0.6 128,175 - +1.4 128,175 - -0.1 128,217 + -4.2 128,431 - +0.6 128,449 + +1.4 128,450 - +2.3 128,450 - +2.4 128,450 - -3.2 128,572 + -1.1 128,598 - +0.1 128,598 - +1.9 128,598 - -3.3 128,629 - ABIE40_RS27050 0.17 +3.6 128,629 - ABIE40_RS27050 0.17 -2.1 128,719 + ABIE40_RS27050 0.40 +2.0 128,720 - ABIE40_RS27050 0.40 -0.7 128,732 + ABIE40_RS27050 0.43 +1.9 128,732 + ABIE40_RS27050 0.43 -0.5 128,733 - ABIE40_RS27050 0.43 +0.4 128,733 - ABIE40_RS27050 0.43 -2.8 128,733 - ABIE40_RS27050 0.43 -3.2 128,933 + -0.5 128,934 - -2.5 128,934 - +0.2 129,322 + ABIE40_RS27055 0.65 -1.9 129,322 + ABIE40_RS27055 0.65 -1.9 129,323 - ABIE40_RS27055 0.66 -1.2 129,392 + ABIE40_RS27055 0.85 -1.0 129,393 - ABIE40_RS27055 0.85 -2.3 129,393 - ABIE40_RS27055 0.85 -3.7 129,393 - ABIE40_RS27055 0.85 -1.1 129,393 - ABIE40_RS27055 0.85 +2.3 129,393 - ABIE40_RS27055 0.85 -1.9 129,432 + -1.5 129,432 + +0.5 129,514 + +0.5 129,515 - +0.6 129,515 - -1.2 129,631 - -1.1 129,637 + -0.8 129,637 + -0.9 129,637 + +1.2 129,638 - +0.1 129,638 - -4.8 129,639 + +0.7 129,639 + +1.0 129,640 - +0.6 129,640 - -2.8 129,640 - -0.1 129,640 - -0.0 129,644 + +0.4 129,645 - -0.4 129,646 + -3.4 129,690 - -1.8 129,870 + +2.0 129,870 + -1.4 129,870 + -0.8 129,870 + -4.0 129,870 + -2.6 129,871 - -2.5 129,871 - -1.3
Or see this region's nucleotide sequence