Experiment: L-Tryptophan (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ABID97_RS01105 and ABID97_RS01110 are separated by 61 nucleotides ABID97_RS01110 and ABID97_RS01115 are separated by 46 nucleotides ABID97_RS01115 and ABID97_RS01120 overlap by 2 nucleotides
ABID97_RS01105: ABID97_RS01105 - AMP-binding protein, at 223,437 to 224,684
_RS01105
ABID97_RS01110: ABID97_RS01110 - citrate/2-methylcitrate synthase, at 224,746 to 225,000
_RS01110
ABID97_RS01115: ABID97_RS01115 - citrate/2-methylcitrate synthase, at 225,047 to 225,946
_RS01115
ABID97_RS01120: ABID97_RS01120 - citrate synthase, at 225,945 to 227,111
_RS01120
Position (kb) 225
226 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1 at 224.130 kb on + strand, within ABID97_RS01105 at 224.131 kb on - strand, within ABID97_RS01105 at 224.677 kb on - strand at 224.677 kb on - strand at 224.934 kb on - strand, within ABID97_RS01110 at 225.109 kb on - strand at 225.361 kb on - strand, within ABID97_RS01115 at 225.873 kb on + strand at 225.913 kb on + strand at 225.914 kb on - strand at 226.086 kb on + strand, within ABID97_RS01120 at 226.825 kb on - strand, within ABID97_RS01120
Per-strain Table
Position Strand Gene LocusTag Fraction L-Tryptophan (C) remove 224,130 + ABID97_RS01105 0.56 -0.5 224,131 - ABID97_RS01105 0.56 -3.8 224,677 - -0.5 224,677 - -0.2 224,934 - ABID97_RS01110 0.74 +0.0 225,109 - -0.3 225,361 - ABID97_RS01115 0.35 +0.8 225,873 + -1.2 225,913 + -2.6 225,914 - -0.2 226,086 + ABID97_RS01120 0.12 -2.2 226,825 - ABID97_RS01120 0.75 -1.8
Or see this region's nucleotide sequence