Strain Fitness in Escherichia coli BW25113 around b1913

Experiment: Gly-DL-Asp (N)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntleuZ and cysT are separated by 12 nucleotidescysT and glyW are separated by 54 nucleotidesglyW and pgsA are separated by 151 nucleotidespgsA and uvrC are separated by 56 nucleotidesuvrC and uvrY overlap by 4 nucleotides b1909: leuZ - tRNA-Leu (NCBI), at 1,989,839 to 1,989,925 leuZ b1910: cysT - tRNA-Cys (NCBI), at 1,989,938 to 1,990,011 cysT b1911: glyW - tRNA-Gly (NCBI), at 1,990,066 to 1,990,141 glyW b1912: pgsA - phosphatidylglycerophosphate synthetase (NCBI), at 1,990,293 to 1,990,841 pgsA b1913: uvrC - excinuclease ABC, subunit C; repair of UV damage to DNA (VIMSS), at 1,990,898 to 1,992,730 uvrC b1914: uvrY - response regulator (NCBI), at 1,992,727 to 1,993,383 uvrY Position (kb) 1990 1991 1992 1993Strain fitness (log2 ratio) -1 0 1at 1990.916 kb on + strandat 1991.220 kb on + strand, within uvrCat 1991.230 kb on + strand, within uvrCat 1991.528 kb on + strand, within uvrCat 1991.533 kb on + strand, within uvrCat 1991.533 kb on + strand, within uvrCat 1991.662 kb on + strand, within uvrCat 1991.662 kb on + strand, within uvrCat 1991.798 kb on - strand, within uvrCat 1991.798 kb on - strand, within uvrCat 1991.857 kb on + strand, within uvrCat 1991.922 kb on + strand, within uvrCat 1991.948 kb on - strand, within uvrCat 1992.150 kb on + strand, within uvrCat 1992.202 kb on + strand, within uvrCat 1992.227 kb on - strand, within uvrCat 1992.461 kb on - strand, within uvrCat 1992.461 kb on - strand, within uvrCat 1992.523 kb on - strand, within uvrCat 1992.523 kb on - strand, within uvrCat 1992.721 kb on + strandat 1992.862 kb on + strand, within uvrYat 1993.136 kb on - strand, within uvrYat 1993.136 kb on - strand, within uvrYat 1993.220 kb on - strand, within uvrYat 1993.236 kb on + strand, within uvrYat 1993.236 kb on + strand, within uvrYat 1993.244 kb on - strand, within uvrYat 1993.244 kb on - strand, within uvrYat 1993.268 kb on - strand, within uvrYat 1993.309 kb on + strand, within uvrYat 1993.317 kb on + strand, within uvrYat 1993.328 kb on + strandat 1993.345 kb on + strandat 1993.350 kb on + strandat 1993.350 kb on + strandat 1993.358 kb on - strandat 1993.415 kb on - strandat 1993.419 kb on + strandat 1993.419 kb on - strandat 1993.522 kb on + strandat 1993.522 kb on + strandat 1993.522 kb on + strandat 1993.522 kb on + strandat 1993.534 kb on + strandat 1993.537 kb on + strandat 1993.540 kb on - strandat 1993.542 kb on - strandat 1993.548 kb on - strandat 1993.548 kb on - strandat 1993.549 kb on + strandat 1993.559 kb on + strandat 1993.559 kb on + strandat 1993.559 kb on + strandat 1993.559 kb on + strandat 1993.559 kb on + strandat 1993.559 kb on + strandat 1993.560 kb on + strandat 1993.560 kb on + strandat 1993.565 kb on + strandat 1993.571 kb on + strandat 1993.571 kb on + strandat 1993.571 kb on + strandat 1993.717 kb on + strandat 1993.717 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Gly-DL-Asp (N)
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1,990,916 + -0.4
1,991,220 + uvrC b1913 0.18 +0.1
1,991,230 + uvrC b1913 0.18 -0.5
1,991,528 + uvrC b1913 0.34 +0.4
1,991,533 + uvrC b1913 0.35 +0.5
1,991,533 + uvrC b1913 0.35 +1.5
1,991,662 + uvrC b1913 0.42 +0.4
1,991,662 + uvrC b1913 0.42 -0.2
1,991,798 - uvrC b1913 0.49 +0.3
1,991,798 - uvrC b1913 0.49 +1.1
1,991,857 + uvrC b1913 0.52 -0.3
1,991,922 + uvrC b1913 0.56 -0.4
1,991,948 - uvrC b1913 0.57 +0.3
1,992,150 + uvrC b1913 0.68 +0.5
1,992,202 + uvrC b1913 0.71 +0.4
1,992,227 - uvrC b1913 0.73 +0.1
1,992,461 - uvrC b1913 0.85 +0.1
1,992,461 - uvrC b1913 0.85 -0.1
1,992,523 - uvrC b1913 0.89 +0.4
1,992,523 - uvrC b1913 0.89 +0.1
1,992,721 + -0.5
1,992,862 + uvrY b1914 0.21 -0.4
1,993,136 - uvrY b1914 0.62 -0.6
1,993,136 - uvrY b1914 0.62 +0.2
1,993,220 - uvrY b1914 0.75 -1.1
1,993,236 + uvrY b1914 0.77 +0.3
1,993,236 + uvrY b1914 0.77 -0.3
1,993,244 - uvrY b1914 0.79 +0.4
1,993,244 - uvrY b1914 0.79 +0.5
1,993,268 - uvrY b1914 0.82 -0.8
1,993,309 + uvrY b1914 0.89 +0.0
1,993,317 + uvrY b1914 0.90 +1.3
1,993,328 + -0.2
1,993,345 + -0.2
1,993,350 + +0.8
1,993,350 + -0.3
1,993,358 - +0.4
1,993,415 - -0.6
1,993,419 + -1.2
1,993,419 - -0.4
1,993,522 + +0.0
1,993,522 + +0.5
1,993,522 + +0.8
1,993,522 + +0.4
1,993,534 + +0.7
1,993,537 + -1.1
1,993,540 - -0.2
1,993,542 - -0.2
1,993,548 - -0.9
1,993,548 - -0.7
1,993,549 + -1.6
1,993,559 + +0.7
1,993,559 + -0.3
1,993,559 + +0.3
1,993,559 + -0.3
1,993,559 + +0.3
1,993,559 + -0.6
1,993,560 + +0.1
1,993,560 + +0.4
1,993,565 + -0.5
1,993,571 + -0.6
1,993,571 + +0.6
1,993,571 + +1.3
1,993,717 + +0.2
1,993,717 + -1.3

Or see this region's nucleotide sequence