Experiment: Manganese (II) chloride tetrahydrate 50 uM, anaerobic
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt IAI46_23240 and IAI46_23245 are separated by 11 nucleotides IAI46_23245 and IAI46_23250 are separated by 7 nucleotides IAI46_23250 and IAI46_23255 are separated by 25 nucleotides IAI46_23255 and IAI46_23260 overlap by 29 nucleotides
IAI46_23240: IAI46_23240 - shikimate dehydrogenase, at 4,967,892 to 4,968,710
_23240
IAI46_23245: IAI46_23245 - L-threonylcarbamoyladenylate synthase type 1 TsaC, at 4,968,722 to 4,969,291
_23245
IAI46_23250: IAI46_23250 - topoisomerase DNA-binding C4 zinc finger domain-containing protein, at 4,969,299 to 4,969,844
_23250
IAI46_23255: IAI46_23255 - DUF494 domain-containing protein, at 4,969,870 to 4,970,343
_23255
IAI46_23260: IAI46_23260 - DNA-protecting protein DprA, at 4,970,315 to 4,971,436
_23260
Position (kb)
4969
4970 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 4968.410 kb on + strand, within IAI46_23240 at 4968.411 kb on - strand, within IAI46_23240 at 4968.506 kb on - strand, within IAI46_23240 at 4968.506 kb on - strand, within IAI46_23240 at 4968.518 kb on + strand, within IAI46_23240 at 4968.519 kb on - strand, within IAI46_23240 at 4968.608 kb on + strand, within IAI46_23240 at 4968.608 kb on + strand, within IAI46_23240 at 4968.609 kb on - strand, within IAI46_23240 at 4968.613 kb on + strand, within IAI46_23240 at 4968.614 kb on - strand, within IAI46_23240 at 4968.614 kb on - strand, within IAI46_23240 at 4968.614 kb on - strand, within IAI46_23240 at 4968.624 kb on + strand, within IAI46_23240 at 4968.668 kb on + strand at 4968.669 kb on - strand at 4968.717 kb on + strand at 4968.723 kb on + strand at 4969.301 kb on - strand at 4969.315 kb on - strand at 4969.390 kb on + strand, within IAI46_23250 at 4969.399 kb on + strand, within IAI46_23250 at 4969.400 kb on - strand, within IAI46_23250 at 4969.483 kb on + strand, within IAI46_23250 at 4969.484 kb on - strand, within IAI46_23250 at 4969.484 kb on - strand, within IAI46_23250 at 4969.607 kb on + strand, within IAI46_23250 at 4969.607 kb on + strand, within IAI46_23250 at 4969.608 kb on - strand, within IAI46_23250 at 4969.682 kb on + strand, within IAI46_23250 at 4969.682 kb on + strand, within IAI46_23250 at 4969.682 kb on + strand, within IAI46_23250 at 4969.683 kb on - strand, within IAI46_23250 at 4969.715 kb on + strand, within IAI46_23250 at 4969.715 kb on + strand, within IAI46_23250 at 4969.715 kb on + strand, within IAI46_23250 at 4969.716 kb on - strand, within IAI46_23250 at 4969.844 kb on + strand at 4969.844 kb on + strand at 4969.845 kb on - strand at 4969.855 kb on - strand at 4969.856 kb on + strand at 4969.857 kb on - strand at 4969.857 kb on - strand at 4969.857 kb on - strand at 4969.857 kb on - strand at 4969.857 kb on - strand at 4969.880 kb on + strand at 4969.891 kb on + strand at 4969.892 kb on - strand at 4969.892 kb on - strand at 4969.934 kb on + strand, within IAI46_23255 at 4969.947 kb on + strand, within IAI46_23255 at 4969.948 kb on - strand, within IAI46_23255 at 4969.998 kb on + strand, within IAI46_23255 at 4970.013 kb on + strand, within IAI46_23255 at 4970.053 kb on + strand, within IAI46_23255 at 4970.127 kb on - strand, within IAI46_23255 at 4970.157 kb on + strand, within IAI46_23255 at 4970.157 kb on + strand, within IAI46_23255 at 4970.158 kb on - strand, within IAI46_23255 at 4970.162 kb on + strand, within IAI46_23255 at 4970.223 kb on - strand, within IAI46_23255 at 4970.310 kb on - strand at 4970.310 kb on - strand at 4970.324 kb on + strand at 4970.325 kb on - strand at 4970.333 kb on - strand at 4970.333 kb on - strand at 4970.333 kb on - strand at 4970.358 kb on - strand at 4970.367 kb on + strand at 4970.368 kb on - strand at 4970.399 kb on + strand at 4970.399 kb on + strand at 4970.399 kb on + strand at 4970.400 kb on - strand at 4970.423 kb on + strand at 4970.423 kb on + strand at 4970.426 kb on + strand at 4970.426 kb on + strand at 4970.427 kb on - strand at 4970.427 kb on - strand at 4970.427 kb on - strand at 4970.427 kb on - strand at 4970.489 kb on + strand, within IAI46_23260 at 4970.489 kb on + strand, within IAI46_23260 at 4970.489 kb on + strand, within IAI46_23260 at 4970.490 kb on - strand, within IAI46_23260 at 4970.490 kb on - strand, within IAI46_23260 at 4970.490 kb on - strand, within IAI46_23260 at 4970.490 kb on - strand, within IAI46_23260 at 4970.545 kb on + strand, within IAI46_23260 at 4970.545 kb on + strand, within IAI46_23260 at 4970.625 kb on + strand, within IAI46_23260 at 4970.626 kb on - strand, within IAI46_23260 at 4970.626 kb on - strand, within IAI46_23260 at 4970.765 kb on + strand, within IAI46_23260 at 4970.765 kb on + strand, within IAI46_23260
Per-strain Table
Position Strand Gene LocusTag Fraction Manganese (II) chloride tetrahydrate 50 uM, anaerobic remove 4,968,410 + IAI46_23240 0.63 +2.0 4,968,411 - IAI46_23240 0.63 +1.5 4,968,506 - IAI46_23240 0.75 -0.7 4,968,506 - IAI46_23240 0.75 -1.7 4,968,518 + IAI46_23240 0.76 +0.0 4,968,519 - IAI46_23240 0.77 -0.9 4,968,608 + IAI46_23240 0.87 +0.1 4,968,608 + IAI46_23240 0.87 +0.0 4,968,609 - IAI46_23240 0.88 -0.7 4,968,613 + IAI46_23240 0.88 -1.0 4,968,614 - IAI46_23240 0.88 +0.7 4,968,614 - IAI46_23240 0.88 -0.8 4,968,614 - IAI46_23240 0.88 -0.7 4,968,624 + IAI46_23240 0.89 -0.7 4,968,668 + +1.3 4,968,669 - +0.3 4,968,717 + +0.9 4,968,723 + -0.1 4,969,301 - +0.2 4,969,315 - -0.4 4,969,390 + IAI46_23250 0.17 -0.1 4,969,399 + IAI46_23250 0.18 -1.7 4,969,400 - IAI46_23250 0.18 -0.6 4,969,483 + IAI46_23250 0.34 -0.0 4,969,484 - IAI46_23250 0.34 -0.2 4,969,484 - IAI46_23250 0.34 +0.1 4,969,607 + IAI46_23250 0.56 +0.5 4,969,607 + IAI46_23250 0.56 -0.5 4,969,608 - IAI46_23250 0.57 -0.2 4,969,682 + IAI46_23250 0.70 -0.5 4,969,682 + IAI46_23250 0.70 -1.2 4,969,682 + IAI46_23250 0.70 +1.3 4,969,683 - IAI46_23250 0.70 +0.1 4,969,715 + IAI46_23250 0.76 +0.0 4,969,715 + IAI46_23250 0.76 +0.7 4,969,715 + IAI46_23250 0.76 +1.5 4,969,716 - IAI46_23250 0.76 +0.1 4,969,844 + +0.3 4,969,844 + -1.5 4,969,845 - -0.0 4,969,855 - -0.2 4,969,856 + -0.5 4,969,857 - -0.1 4,969,857 - +0.2 4,969,857 - +1.0 4,969,857 - +0.3 4,969,857 - -0.5 4,969,880 + -0.1 4,969,891 + -0.6 4,969,892 - -0.9 4,969,892 - +0.9 4,969,934 + IAI46_23255 0.14 +1.1 4,969,947 + IAI46_23255 0.16 +1.1 4,969,948 - IAI46_23255 0.16 +0.9 4,969,998 + IAI46_23255 0.27 +0.5 4,970,013 + IAI46_23255 0.30 -0.1 4,970,053 + IAI46_23255 0.39 -0.3 4,970,127 - IAI46_23255 0.54 -0.7 4,970,157 + IAI46_23255 0.61 -0.4 4,970,157 + IAI46_23255 0.61 +0.4 4,970,158 - IAI46_23255 0.61 +0.4 4,970,162 + IAI46_23255 0.62 -0.0 4,970,223 - IAI46_23255 0.74 +0.1 4,970,310 - +0.2 4,970,310 - -0.1 4,970,324 + +0.4 4,970,325 - +0.3 4,970,333 - +0.3 4,970,333 - +0.5 4,970,333 - -0.1 4,970,358 - +0.9 4,970,367 + -0.1 4,970,368 - -1.8 4,970,399 + -0.7 4,970,399 + -0.4 4,970,399 + -0.4 4,970,400 - -1.1 4,970,423 + +0.5 4,970,423 + +0.3 4,970,426 + -0.7 4,970,426 + -0.0 4,970,427 - -0.1 4,970,427 - +0.9 4,970,427 - +0.2 4,970,427 - +0.1 4,970,489 + IAI46_23260 0.16 +1.2 4,970,489 + IAI46_23260 0.16 -0.1 4,970,489 + IAI46_23260 0.16 -0.3 4,970,490 - IAI46_23260 0.16 +1.3 4,970,490 - IAI46_23260 0.16 +0.1 4,970,490 - IAI46_23260 0.16 -1.2 4,970,490 - IAI46_23260 0.16 -0.6 4,970,545 + IAI46_23260 0.20 +1.1 4,970,545 + IAI46_23260 0.20 -0.3 4,970,625 + IAI46_23260 0.28 -0.1 4,970,626 - IAI46_23260 0.28 +0.0 4,970,626 - IAI46_23260 0.28 +0.3 4,970,765 + IAI46_23260 0.40 +0.7 4,970,765 + IAI46_23260 0.40 +0.0
Or see this region's nucleotide sequence