Experiment: Manganese (II) chloride tetrahydrate 50 uM, anaerobic
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt IAI46_19340 and IAI46_19345 are separated by 62 nucleotides IAI46_19345 and IAI46_19350 are separated by 125 nucleotides IAI46_19350 and IAI46_19355 are separated by 24 nucleotides IAI46_19355 and IAI46_19360 are separated by 55 nucleotides IAI46_19360 and IAI46_19365 are separated by 195 nucleotides
IAI46_19340: IAI46_19340 - co-chaperone HscB, at 4,082,627 to 4,083,148
_19340
IAI46_19345: IAI46_19345 - iron-sulfur cluster assembly protein IscA, at 4,083,211 to 4,083,534
_19345
IAI46_19350: IAI46_19350 - Fe-S cluster assembly scaffold IscU, at 4,083,660 to 4,084,046
_19350
IAI46_19355: IAI46_19355 - IscS subfamily cysteine desulfurase, at 4,084,071 to 4,085,285
_19355
IAI46_19360: IAI46_19360 - Fe-S cluster assembly transcriptional regulator IscR, at 4,085,341 to 4,085,835
_19360
IAI46_19365: IAI46_19365 - tRNA (cytosine(32)/uridine(32)-2'-O)-methyltransferase TrmJ, at 4,086,031 to 4,086,765
_19365
Position (kb)
4084
4085
4086 Strain fitness (log2 ratio)
-1
0
1
2 at 4083.112 kb on - strand at 4083.136 kb on + strand at 4083.136 kb on + strand at 4083.137 kb on - strand at 4083.141 kb on + strand at 4083.148 kb on + strand at 4083.324 kb on - strand, within IAI46_19345 at 4083.324 kb on - strand, within IAI46_19345 at 4083.417 kb on - strand, within IAI46_19345 at 4083.486 kb on + strand, within IAI46_19345 at 4083.486 kb on + strand, within IAI46_19345 at 4083.487 kb on - strand, within IAI46_19345 at 4083.553 kb on - strand at 4083.598 kb on - strand at 4083.608 kb on + strand at 4083.609 kb on - strand at 4083.609 kb on - strand at 4083.610 kb on + strand at 4083.639 kb on + strand at 4083.639 kb on + strand at 4083.640 kb on - strand at 4083.661 kb on + strand at 4083.661 kb on + strand at 4083.671 kb on + strand at 4083.685 kb on + strand at 4083.704 kb on + strand, within IAI46_19350 at 4083.725 kb on + strand, within IAI46_19350 at 4083.725 kb on + strand, within IAI46_19350 at 4083.726 kb on - strand, within IAI46_19350 at 4083.872 kb on + strand, within IAI46_19350 at 4083.873 kb on - strand, within IAI46_19350 at 4083.957 kb on - strand, within IAI46_19350 at 4083.957 kb on - strand, within IAI46_19350 at 4083.972 kb on - strand, within IAI46_19350 at 4083.972 kb on - strand, within IAI46_19350 at 4084.039 kb on + strand at 4084.039 kb on + strand at 4084.072 kb on + strand at 4084.073 kb on - strand at 4084.073 kb on - strand at 4084.101 kb on - strand at 4084.121 kb on + strand at 4084.122 kb on - strand at 4084.122 kb on - strand at 4084.124 kb on - strand at 4084.163 kb on + strand at 4084.163 kb on + strand at 4084.164 kb on - strand at 4084.164 kb on - strand at 4084.164 kb on - strand at 4085.302 kb on - strand at 4085.302 kb on - strand at 4085.302 kb on - strand at 4085.342 kb on + strand at 4085.356 kb on - strand at 4085.356 kb on - strand at 4085.364 kb on + strand at 4085.464 kb on - strand, within IAI46_19360 at 4085.599 kb on - strand, within IAI46_19360 at 4085.702 kb on - strand, within IAI46_19360 at 4085.702 kb on - strand, within IAI46_19360 at 4085.715 kb on - strand, within IAI46_19360 at 4085.728 kb on - strand, within IAI46_19360 at 4085.797 kb on - strand at 4085.797 kb on - strand at 4085.821 kb on - strand at 4085.821 kb on - strand at 4085.821 kb on - strand at 4085.839 kb on + strand at 4085.840 kb on - strand at 4085.840 kb on - strand at 4085.840 kb on - strand at 4085.912 kb on - strand at 4085.946 kb on + strand at 4085.947 kb on - strand at 4085.977 kb on + strand at 4085.991 kb on - strand at 4085.994 kb on + strand at 4085.995 kb on - strand at 4086.000 kb on + strand at 4086.003 kb on + strand at 4086.133 kb on + strand, within IAI46_19365
Per-strain Table
Position Strand Gene LocusTag Fraction Manganese (II) chloride tetrahydrate 50 uM, anaerobic remove 4,083,112 - -0.8 4,083,136 + -0.9 4,083,136 + -0.0 4,083,137 - +0.0 4,083,141 + -1.1 4,083,148 + -0.2 4,083,324 - IAI46_19345 0.35 +1.1 4,083,324 - IAI46_19345 0.35 -0.5 4,083,417 - IAI46_19345 0.64 +2.3 4,083,486 + IAI46_19345 0.85 -1.4 4,083,486 + IAI46_19345 0.85 +0.2 4,083,487 - IAI46_19345 0.85 -0.0 4,083,553 - -0.5 4,083,598 - -0.2 4,083,608 + -0.8 4,083,609 - +0.6 4,083,609 - -0.0 4,083,610 + -0.8 4,083,639 + -0.2 4,083,639 + -0.6 4,083,640 - +0.3 4,083,661 + +0.0 4,083,661 + -0.1 4,083,671 + +0.1 4,083,685 + -1.2 4,083,704 + IAI46_19350 0.11 -1.6 4,083,725 + IAI46_19350 0.17 -0.1 4,083,725 + IAI46_19350 0.17 -0.4 4,083,726 - IAI46_19350 0.17 +0.6 4,083,872 + IAI46_19350 0.55 +0.5 4,083,873 - IAI46_19350 0.55 -0.8 4,083,957 - IAI46_19350 0.77 -0.6 4,083,957 - IAI46_19350 0.77 +0.6 4,083,972 - IAI46_19350 0.81 -0.5 4,083,972 - IAI46_19350 0.81 -0.7 4,084,039 + -1.2 4,084,039 + -0.1 4,084,072 + -1.4 4,084,073 - -1.7 4,084,073 - +0.0 4,084,101 - +0.6 4,084,121 + +0.7 4,084,122 - -0.3 4,084,122 - -0.8 4,084,124 - +0.9 4,084,163 + -0.6 4,084,163 + -1.6 4,084,164 - +0.2 4,084,164 - -0.3 4,084,164 - -0.5 4,085,302 - -0.6 4,085,302 - -0.2 4,085,302 - +1.8 4,085,342 + +0.5 4,085,356 - +0.1 4,085,356 - -0.7 4,085,364 + -1.6 4,085,464 - IAI46_19360 0.25 +0.5 4,085,599 - IAI46_19360 0.52 +0.7 4,085,702 - IAI46_19360 0.73 +1.0 4,085,702 - IAI46_19360 0.73 +0.8 4,085,715 - IAI46_19360 0.76 -0.2 4,085,728 - IAI46_19360 0.78 +0.3 4,085,797 - -1.6 4,085,797 - +0.2 4,085,821 - +0.4 4,085,821 - +1.3 4,085,821 - +0.1 4,085,839 + -0.8 4,085,840 - +1.1 4,085,840 - +0.6 4,085,840 - +1.0 4,085,912 - -0.7 4,085,946 + +0.6 4,085,947 - -0.9 4,085,977 + -0.1 4,085,991 - -0.1 4,085,994 + -0.6 4,085,995 - +0.6 4,086,000 + -0.1 4,086,003 + -0.1 4,086,133 + IAI46_19365 0.14 -0.0
Or see this region's nucleotide sequence