Experiment: Manganese (II) chloride tetrahydrate 50 uM, anaerobic
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt IAI46_17865 and IAI46_17870 overlap by 14 nucleotides IAI46_17870 and IAI46_17875 are separated by 220 nucleotides IAI46_17875 and IAI46_17880 are separated by 299 nucleotides
IAI46_17865: IAI46_17865 - sensor histidine kinase N-terminal domain-containing protein, at 3,764,363 to 3,765,781
_17865
IAI46_17870: IAI46_17870 - transcriptional regulator TctD, at 3,765,768 to 3,766,442
_17870
IAI46_17875: IAI46_17875 - hypothetical protein, at 3,766,663 to 3,766,989
_17875
IAI46_17880: IAI46_17880 - tripartite tricarboxylate transporter substrate binding protein, at 3,767,289 to 3,768,269
_17880
Position (kb)
3765
3766
3767 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 3764.868 kb on + strand, within IAI46_17865 at 3764.951 kb on - strand, within IAI46_17865 at 3765.070 kb on + strand, within IAI46_17865 at 3765.071 kb on - strand, within IAI46_17865 at 3765.071 kb on - strand, within IAI46_17865 at 3765.164 kb on - strand, within IAI46_17865 at 3765.190 kb on + strand, within IAI46_17865 at 3765.191 kb on - strand, within IAI46_17865 at 3765.233 kb on + strand, within IAI46_17865 at 3765.288 kb on + strand, within IAI46_17865 at 3765.288 kb on + strand, within IAI46_17865 at 3765.478 kb on - strand, within IAI46_17865 at 3765.488 kb on - strand, within IAI46_17865 at 3765.491 kb on + strand, within IAI46_17865 at 3765.491 kb on + strand, within IAI46_17865 at 3765.491 kb on + strand, within IAI46_17865 at 3765.492 kb on - strand, within IAI46_17865 at 3765.492 kb on - strand, within IAI46_17865 at 3765.516 kb on + strand, within IAI46_17865 at 3765.517 kb on - strand, within IAI46_17865 at 3765.519 kb on + strand, within IAI46_17865 at 3765.520 kb on - strand, within IAI46_17865 at 3765.564 kb on + strand, within IAI46_17865 at 3765.565 kb on - strand, within IAI46_17865 at 3765.565 kb on - strand, within IAI46_17865 at 3765.565 kb on - strand, within IAI46_17865 at 3765.648 kb on + strand at 3765.648 kb on + strand at 3765.649 kb on - strand at 3765.681 kb on + strand at 3765.681 kb on + strand at 3765.682 kb on - strand at 3765.707 kb on + strand at 3765.707 kb on + strand at 3765.753 kb on - strand at 3765.770 kb on - strand at 3765.868 kb on + strand, within IAI46_17870 at 3765.868 kb on + strand, within IAI46_17870 at 3765.868 kb on + strand, within IAI46_17870 at 3765.869 kb on - strand, within IAI46_17870 at 3765.869 kb on - strand, within IAI46_17870 at 3765.869 kb on - strand, within IAI46_17870 at 3765.869 kb on - strand, within IAI46_17870 at 3766.058 kb on - strand, within IAI46_17870 at 3766.178 kb on + strand, within IAI46_17870 at 3766.224 kb on + strand, within IAI46_17870 at 3766.224 kb on + strand, within IAI46_17870 at 3766.224 kb on + strand, within IAI46_17870 at 3766.224 kb on + strand, within IAI46_17870 at 3766.225 kb on - strand, within IAI46_17870 at 3766.225 kb on - strand, within IAI46_17870 at 3766.407 kb on + strand at 3766.529 kb on + strand at 3766.530 kb on - strand at 3766.530 kb on - strand at 3766.562 kb on + strand at 3766.562 kb on + strand at 3766.562 kb on + strand at 3766.563 kb on - strand at 3766.617 kb on + strand at 3766.617 kb on + strand at 3766.618 kb on - strand at 3766.644 kb on + strand at 3766.664 kb on + strand at 3766.665 kb on - strand at 3766.665 kb on - strand at 3766.726 kb on - strand, within IAI46_17875 at 3766.812 kb on - strand, within IAI46_17875 at 3766.876 kb on - strand, within IAI46_17875 at 3766.913 kb on + strand, within IAI46_17875 at 3766.914 kb on - strand, within IAI46_17875 at 3766.990 kb on - strand at 3766.992 kb on + strand at 3766.993 kb on - strand at 3766.993 kb on - strand at 3767.046 kb on + strand at 3767.058 kb on + strand at 3767.058 kb on + strand at 3767.080 kb on + strand at 3767.091 kb on - strand at 3767.091 kb on - strand at 3767.145 kb on + strand at 3767.155 kb on + strand at 3767.169 kb on + strand at 3767.170 kb on - strand at 3767.170 kb on - strand at 3767.170 kb on - strand at 3767.226 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Manganese (II) chloride tetrahydrate 50 uM, anaerobic remove 3,764,868 + IAI46_17865 0.36 +0.4 3,764,951 - IAI46_17865 0.41 +0.8 3,765,070 + IAI46_17865 0.50 +0.1 3,765,071 - IAI46_17865 0.50 +0.3 3,765,071 - IAI46_17865 0.50 +0.1 3,765,164 - IAI46_17865 0.56 -0.5 3,765,190 + IAI46_17865 0.58 -1.2 3,765,191 - IAI46_17865 0.58 +0.6 3,765,233 + IAI46_17865 0.61 -0.0 3,765,288 + IAI46_17865 0.65 -1.7 3,765,288 + IAI46_17865 0.65 -0.9 3,765,478 - IAI46_17865 0.79 -0.6 3,765,488 - IAI46_17865 0.79 -0.1 3,765,491 + IAI46_17865 0.79 -0.3 3,765,491 + IAI46_17865 0.79 -0.5 3,765,491 + IAI46_17865 0.79 +0.3 3,765,492 - IAI46_17865 0.80 +0.2 3,765,492 - IAI46_17865 0.80 -0.6 3,765,516 + IAI46_17865 0.81 +0.6 3,765,517 - IAI46_17865 0.81 -0.9 3,765,519 + IAI46_17865 0.81 +1.0 3,765,520 - IAI46_17865 0.82 -0.4 3,765,564 + IAI46_17865 0.85 -0.3 3,765,565 - IAI46_17865 0.85 -0.2 3,765,565 - IAI46_17865 0.85 +0.3 3,765,565 - IAI46_17865 0.85 -0.1 3,765,648 + +0.6 3,765,648 + -1.2 3,765,649 - -1.9 3,765,681 + +0.8 3,765,681 + +0.3 3,765,682 - +1.3 3,765,707 + -0.2 3,765,707 + +1.1 3,765,753 - +0.3 3,765,770 - +0.7 3,765,868 + IAI46_17870 0.15 +1.0 3,765,868 + IAI46_17870 0.15 +0.4 3,765,868 + IAI46_17870 0.15 +0.3 3,765,869 - IAI46_17870 0.15 +0.4 3,765,869 - IAI46_17870 0.15 +1.7 3,765,869 - IAI46_17870 0.15 +0.7 3,765,869 - IAI46_17870 0.15 -0.5 3,766,058 - IAI46_17870 0.43 +1.9 3,766,178 + IAI46_17870 0.61 -0.4 3,766,224 + IAI46_17870 0.68 +0.3 3,766,224 + IAI46_17870 0.68 -0.6 3,766,224 + IAI46_17870 0.68 +0.7 3,766,224 + IAI46_17870 0.68 -1.0 3,766,225 - IAI46_17870 0.68 +0.7 3,766,225 - IAI46_17870 0.68 -1.7 3,766,407 + +0.4 3,766,529 + +1.2 3,766,530 - -0.7 3,766,530 - +0.0 3,766,562 + +0.2 3,766,562 + +0.5 3,766,562 + -0.1 3,766,563 - +0.3 3,766,617 + +0.7 3,766,617 + -1.1 3,766,618 - -0.1 3,766,644 + +0.5 3,766,664 + +0.9 3,766,665 - -0.1 3,766,665 - -0.1 3,766,726 - IAI46_17875 0.19 +0.2 3,766,812 - IAI46_17875 0.46 -0.4 3,766,876 - IAI46_17875 0.65 -0.9 3,766,913 + IAI46_17875 0.76 +0.6 3,766,914 - IAI46_17875 0.77 -0.7 3,766,990 - -0.2 3,766,992 + +0.2 3,766,993 - +0.3 3,766,993 - -0.7 3,767,046 + +1.6 3,767,058 + +1.2 3,767,058 + +0.9 3,767,080 + -0.2 3,767,091 - +1.3 3,767,091 - +0.6 3,767,145 + +0.1 3,767,155 + -1.8 3,767,169 + -0.2 3,767,170 - -0.8 3,767,170 - -0.3 3,767,170 - +0.9 3,767,226 - +0.8
Or see this region's nucleotide sequence