Experiment: Manganese (II) chloride tetrahydrate 50 uM, anaerobic
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt IAI46_13170 and IAI46_13175 are separated by 41 nucleotides IAI46_13175 and IAI46_13180 are separated by 73 nucleotides IAI46_13180 and IAI46_13185 are separated by 99 nucleotides
IAI46_13170: IAI46_13170 - pyridoxal kinase, at 2,808,655 to 2,809,494
_13170
IAI46_13175: IAI46_13175 - flagellar brake protein, at 2,809,536 to 2,810,249
_13175
IAI46_13180: IAI46_13180 - N-formylglutamate amidohydrolase, at 2,810,323 to 2,811,105
_13180
IAI46_13185: IAI46_13185 - ornithine lipid synthase OlsF, at 2,811,205 to 2,812,926
_13185
Position (kb)
2810
2811
2812 Strain fitness (log2 ratio)
-2
-1
0
1 at 2809.846 kb on + strand, within IAI46_13175 at 2809.865 kb on - strand, within IAI46_13175 at 2810.050 kb on + strand, within IAI46_13175 at 2810.050 kb on + strand, within IAI46_13175 at 2810.223 kb on - strand at 2810.435 kb on + strand, within IAI46_13180 at 2810.474 kb on + strand, within IAI46_13180 at 2810.475 kb on - strand, within IAI46_13180 at 2810.475 kb on - strand, within IAI46_13180 at 2810.711 kb on + strand, within IAI46_13180 at 2810.855 kb on + strand, within IAI46_13180 at 2810.892 kb on + strand, within IAI46_13180 at 2810.892 kb on + strand, within IAI46_13180 at 2810.934 kb on + strand, within IAI46_13180 at 2810.944 kb on + strand, within IAI46_13180 at 2811.017 kb on - strand, within IAI46_13180 at 2811.134 kb on + strand at 2811.135 kb on - strand at 2811.135 kb on - strand at 2811.139 kb on + strand at 2811.139 kb on + strand at 2811.139 kb on + strand at 2811.139 kb on + strand at 2811.144 kb on + strand at 2811.212 kb on - strand at 2811.212 kb on - strand at 2811.212 kb on - strand at 2811.268 kb on - strand at 2811.303 kb on + strand at 2811.303 kb on + strand at 2811.304 kb on - strand at 2811.309 kb on + strand at 2811.372 kb on + strand at 2811.373 kb on - strand at 2811.374 kb on + strand at 2811.374 kb on + strand at 2811.375 kb on - strand at 2811.381 kb on + strand, within IAI46_13185 at 2811.383 kb on + strand, within IAI46_13185 at 2811.384 kb on - strand, within IAI46_13185 at 2811.618 kb on - strand, within IAI46_13185 at 2811.623 kb on + strand, within IAI46_13185 at 2811.742 kb on - strand, within IAI46_13185 at 2811.782 kb on + strand, within IAI46_13185 at 2811.900 kb on - strand, within IAI46_13185
Per-strain Table
Position Strand Gene LocusTag Fraction Manganese (II) chloride tetrahydrate 50 uM, anaerobic remove 2,809,846 + IAI46_13175 0.43 +0.5 2,809,865 - IAI46_13175 0.46 -0.1 2,810,050 + IAI46_13175 0.72 -1.2 2,810,050 + IAI46_13175 0.72 +0.0 2,810,223 - -0.5 2,810,435 + IAI46_13180 0.14 -2.1 2,810,474 + IAI46_13180 0.19 -1.5 2,810,475 - IAI46_13180 0.19 +0.6 2,810,475 - IAI46_13180 0.19 +0.4 2,810,711 + IAI46_13180 0.50 -0.1 2,810,855 + IAI46_13180 0.68 -0.3 2,810,892 + IAI46_13180 0.73 +0.8 2,810,892 + IAI46_13180 0.73 +0.3 2,810,934 + IAI46_13180 0.78 -1.1 2,810,944 + IAI46_13180 0.79 -0.8 2,811,017 - IAI46_13180 0.89 +1.1 2,811,134 + +1.5 2,811,135 - -0.7 2,811,135 - +0.1 2,811,139 + -1.1 2,811,139 + +1.2 2,811,139 + -0.8 2,811,139 + +1.0 2,811,144 + +0.1 2,811,212 - +0.2 2,811,212 - +0.2 2,811,212 - +1.0 2,811,268 - -0.0 2,811,303 + -0.3 2,811,303 + +1.5 2,811,304 - -0.4 2,811,309 + -1.0 2,811,372 + -1.0 2,811,373 - -0.8 2,811,374 + +0.0 2,811,374 + +1.0 2,811,375 - +1.0 2,811,381 + IAI46_13185 0.10 -0.1 2,811,383 + IAI46_13185 0.10 +1.5 2,811,384 - IAI46_13185 0.10 -1.3 2,811,618 - IAI46_13185 0.24 -0.0 2,811,623 + IAI46_13185 0.24 -0.8 2,811,742 - IAI46_13185 0.31 +0.7 2,811,782 + IAI46_13185 0.34 +0.2 2,811,900 - IAI46_13185 0.40 -0.8
Or see this region's nucleotide sequence