Experiment: Manganese (II) chloride tetrahydrate 50 uM, anaerobic
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt IAI46_09205 and IAI46_09210 overlap by 8 nucleotides IAI46_09210 and IAI46_09215 are separated by 58 nucleotides IAI46_09215 and IAI46_09220 are separated by 145 nucleotides IAI46_09220 and IAI46_09225 are separated by 58 nucleotides IAI46_09225 and IAI46_09230 are separated by 10 nucleotides
IAI46_09205: IAI46_09205 - UvrY/SirA/GacA family response regulator transcription factor, at 1,971,189 to 1,971,845
_09205
IAI46_09210: IAI46_09210 - excinuclease ABC subunit UvrC, at 1,971,838 to 1,973,670
_09210
IAI46_09215: IAI46_09215 - CDP-diacylglycerol--glycerol-3-phosphate 3-phosphatidyltransferase, at 1,973,729 to 1,974,277
_09215
IAI46_09220: IAI46_09220 - tRNA-Gly, at 1,974,423 to 1,974,498
_09220
IAI46_09225: IAI46_09225 - tRNA-Cys, at 1,974,557 to 1,974,630
_09225
IAI46_09230: IAI46_09230 - tRNA-Leu, at 1,974,641 to 1,974,726
_09230
Position (kb)
1971
1972
1973
1974 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1970.934 kb on - strand at 1970.959 kb on - strand at 1970.991 kb on + strand at 1971.079 kb on + strand at 1971.090 kb on - strand at 1971.090 kb on - strand at 1971.128 kb on + strand at 1971.128 kb on + strand at 1971.128 kb on + strand at 1971.132 kb on - strand at 1971.141 kb on + strand at 1971.141 kb on + strand at 1971.141 kb on + strand at 1971.142 kb on - strand at 1971.143 kb on + strand at 1971.332 kb on - strand, within IAI46_09205 at 1971.335 kb on + strand, within IAI46_09205 at 1971.335 kb on + strand, within IAI46_09205 at 1971.335 kb on + strand, within IAI46_09205 at 1971.350 kb on + strand, within IAI46_09205 at 1971.558 kb on + strand, within IAI46_09205 at 1971.558 kb on + strand, within IAI46_09205 at 1971.560 kb on + strand, within IAI46_09205 at 1971.560 kb on + strand, within IAI46_09205 at 1971.560 kb on + strand, within IAI46_09205 at 1971.657 kb on - strand, within IAI46_09205 at 1971.657 kb on - strand, within IAI46_09205 at 1971.832 kb on + strand at 1971.899 kb on - strand at 1971.903 kb on + strand at 1971.903 kb on + strand at 1971.904 kb on - strand at 1971.904 kb on - strand at 1971.904 kb on - strand at 1971.904 kb on - strand at 1971.907 kb on + strand at 1971.907 kb on + strand at 1971.908 kb on - strand at 1971.908 kb on - strand at 1971.931 kb on + strand at 1971.931 kb on + strand at 1971.932 kb on - strand at 1972.035 kb on - strand, within IAI46_09210 at 1972.041 kb on - strand, within IAI46_09210 at 1972.054 kb on - strand, within IAI46_09210 at 1972.073 kb on + strand, within IAI46_09210 at 1972.083 kb on + strand, within IAI46_09210 at 1972.091 kb on + strand, within IAI46_09210 at 1972.113 kb on + strand, within IAI46_09210 at 1972.244 kb on - strand, within IAI46_09210 at 1972.417 kb on + strand, within IAI46_09210 at 1972.454 kb on + strand, within IAI46_09210 at 1972.457 kb on + strand, within IAI46_09210 at 1972.458 kb on - strand, within IAI46_09210 at 1972.664 kb on + strand, within IAI46_09210 at 1972.747 kb on + strand, within IAI46_09210 at 1972.747 kb on + strand, within IAI46_09210 at 1972.751 kb on + strand, within IAI46_09210 at 1972.790 kb on + strand, within IAI46_09210 at 1972.898 kb on - strand, within IAI46_09210 at 1972.903 kb on - strand, within IAI46_09210 at 1972.903 kb on - strand, within IAI46_09210 at 1972.914 kb on + strand, within IAI46_09210 at 1973.078 kb on + strand, within IAI46_09210 at 1973.119 kb on + strand, within IAI46_09210 at 1973.121 kb on + strand, within IAI46_09210 at 1973.122 kb on - strand, within IAI46_09210 at 1973.122 kb on - strand, within IAI46_09210 at 1973.122 kb on - strand, within IAI46_09210 at 1973.124 kb on + strand, within IAI46_09210 at 1973.152 kb on + strand, within IAI46_09210 at 1973.152 kb on + strand, within IAI46_09210 at 1973.153 kb on - strand, within IAI46_09210 at 1973.223 kb on + strand, within IAI46_09210 at 1973.224 kb on - strand, within IAI46_09210 at 1973.224 kb on - strand, within IAI46_09210 at 1973.270 kb on + strand, within IAI46_09210 at 1973.270 kb on + strand, within IAI46_09210 at 1973.493 kb on - strand at 1973.560 kb on + strand at 1973.561 kb on - strand at 1973.562 kb on + strand at 1973.563 kb on - strand at 1973.582 kb on + strand at 1973.687 kb on + strand at 1973.713 kb on + strand at 1973.714 kb on - strand at 1973.717 kb on + strand at 1973.728 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Manganese (II) chloride tetrahydrate 50 uM, anaerobic remove 1,970,934 - -0.6 1,970,959 - -1.3 1,970,991 + -0.0 1,971,079 + -0.9 1,971,090 - -0.2 1,971,090 - -0.1 1,971,128 + -0.1 1,971,128 + +0.9 1,971,128 + -1.1 1,971,132 - +0.4 1,971,141 + +0.3 1,971,141 + +0.5 1,971,141 + -0.1 1,971,142 - -1.1 1,971,143 + -0.0 1,971,332 - IAI46_09205 0.22 +0.3 1,971,335 + IAI46_09205 0.22 -0.7 1,971,335 + IAI46_09205 0.22 +1.1 1,971,335 + IAI46_09205 0.22 -0.7 1,971,350 + IAI46_09205 0.25 -0.1 1,971,558 + IAI46_09205 0.56 +0.2 1,971,558 + IAI46_09205 0.56 -0.3 1,971,560 + IAI46_09205 0.56 +0.6 1,971,560 + IAI46_09205 0.56 -0.5 1,971,560 + IAI46_09205 0.56 +0.5 1,971,657 - IAI46_09205 0.71 +0.4 1,971,657 - IAI46_09205 0.71 +0.3 1,971,832 + +0.0 1,971,899 - -0.8 1,971,903 + +1.4 1,971,903 + +1.2 1,971,904 - +0.0 1,971,904 - +0.1 1,971,904 - +1.7 1,971,904 - +0.3 1,971,907 + -0.0 1,971,907 + +1.6 1,971,908 - +0.1 1,971,908 - -1.1 1,971,931 + -0.1 1,971,931 + +0.3 1,971,932 - +0.9 1,972,035 - IAI46_09210 0.11 +0.7 1,972,041 - IAI46_09210 0.11 +1.4 1,972,054 - IAI46_09210 0.12 +0.1 1,972,073 + IAI46_09210 0.13 +0.1 1,972,083 + IAI46_09210 0.13 +0.2 1,972,091 + IAI46_09210 0.14 -0.1 1,972,113 + IAI46_09210 0.15 -0.9 1,972,244 - IAI46_09210 0.22 -1.6 1,972,417 + IAI46_09210 0.32 +0.1 1,972,454 + IAI46_09210 0.34 -0.3 1,972,457 + IAI46_09210 0.34 +0.0 1,972,458 - IAI46_09210 0.34 -1.4 1,972,664 + IAI46_09210 0.45 +1.4 1,972,747 + IAI46_09210 0.50 -0.4 1,972,747 + IAI46_09210 0.50 -0.0 1,972,751 + IAI46_09210 0.50 -1.0 1,972,790 + IAI46_09210 0.52 -0.6 1,972,898 - IAI46_09210 0.58 -0.4 1,972,903 - IAI46_09210 0.58 -0.9 1,972,903 - IAI46_09210 0.58 -0.5 1,972,914 + IAI46_09210 0.59 -0.4 1,973,078 + IAI46_09210 0.68 -0.0 1,973,119 + IAI46_09210 0.70 +0.5 1,973,121 + IAI46_09210 0.70 +0.3 1,973,122 - IAI46_09210 0.70 +0.1 1,973,122 - IAI46_09210 0.70 +0.6 1,973,122 - IAI46_09210 0.70 -2.6 1,973,124 + IAI46_09210 0.70 +0.2 1,973,152 + IAI46_09210 0.72 -0.6 1,973,152 + IAI46_09210 0.72 +0.7 1,973,153 - IAI46_09210 0.72 +0.3 1,973,223 + IAI46_09210 0.76 +1.9 1,973,224 - IAI46_09210 0.76 -0.3 1,973,224 - IAI46_09210 0.76 +0.3 1,973,270 + IAI46_09210 0.78 +2.4 1,973,270 + IAI46_09210 0.78 +1.0 1,973,493 - +0.6 1,973,560 + +0.1 1,973,561 - -0.1 1,973,562 + +0.6 1,973,563 - -0.6 1,973,582 + -0.3 1,973,687 + -0.2 1,973,713 + +0.7 1,973,714 - +0.5 1,973,717 + -0.2 1,973,728 + +0.5
Or see this region's nucleotide sequence