Experiment: Manganese (II) chloride tetrahydrate 50 uM, anaerobic
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt IAI46_07215 and IAI46_07220 are separated by 54 nucleotides IAI46_07220 and IAI46_07225 are separated by 173 nucleotides
IAI46_07215: IAI46_07215 - urea carboxylase, at 1,527,832 to 1,531,455
_07215
IAI46_07220: IAI46_07220 - GntR family transcriptional regulator, at 1,531,510 to 1,532,208
_07220
IAI46_07225: IAI46_07225 - urea ABC transporter substrate-binding protein, at 1,532,382 to 1,533,650
_07225
Position (kb)
1531
1532
1533 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1530.695 kb on - strand, within IAI46_07215 at 1530.721 kb on + strand, within IAI46_07215 at 1530.781 kb on + strand, within IAI46_07215 at 1530.835 kb on + strand, within IAI46_07215 at 1530.835 kb on + strand, within IAI46_07215 at 1530.835 kb on + strand, within IAI46_07215 at 1530.835 kb on + strand, within IAI46_07215 at 1530.835 kb on + strand, within IAI46_07215 at 1530.835 kb on + strand, within IAI46_07215 at 1530.835 kb on + strand, within IAI46_07215 at 1530.836 kb on - strand, within IAI46_07215 at 1530.836 kb on - strand, within IAI46_07215 at 1530.847 kb on + strand, within IAI46_07215 at 1530.847 kb on + strand, within IAI46_07215 at 1530.848 kb on - strand, within IAI46_07215 at 1530.848 kb on - strand, within IAI46_07215 at 1530.848 kb on - strand, within IAI46_07215 at 1530.888 kb on + strand, within IAI46_07215 at 1530.888 kb on + strand, within IAI46_07215 at 1530.889 kb on - strand, within IAI46_07215 at 1531.524 kb on + strand at 1531.563 kb on + strand at 1531.563 kb on + strand at 1531.563 kb on + strand at 1531.563 kb on + strand at 1531.563 kb on + strand at 1531.563 kb on + strand at 1531.564 kb on - strand at 1531.564 kb on - strand at 1531.567 kb on + strand at 1531.567 kb on + strand at 1531.568 kb on - strand at 1531.648 kb on - strand, within IAI46_07220 at 1531.706 kb on - strand, within IAI46_07220 at 1531.706 kb on - strand, within IAI46_07220 at 1531.706 kb on - strand, within IAI46_07220 at 1531.709 kb on + strand, within IAI46_07220 at 1531.709 kb on + strand, within IAI46_07220 at 1531.709 kb on + strand, within IAI46_07220 at 1531.710 kb on - strand, within IAI46_07220 at 1531.710 kb on - strand, within IAI46_07220 at 1531.760 kb on - strand, within IAI46_07220 at 1531.774 kb on + strand, within IAI46_07220 at 1531.774 kb on + strand, within IAI46_07220 at 1531.775 kb on - strand, within IAI46_07220 at 1531.775 kb on - strand, within IAI46_07220 at 1531.775 kb on - strand, within IAI46_07220 at 1531.775 kb on - strand, within IAI46_07220 at 1531.775 kb on - strand, within IAI46_07220 at 1531.775 kb on - strand, within IAI46_07220 at 1531.935 kb on + strand, within IAI46_07220 at 1532.056 kb on + strand, within IAI46_07220 at 1532.057 kb on - strand, within IAI46_07220 at 1532.057 kb on - strand, within IAI46_07220 at 1532.134 kb on - strand, within IAI46_07220 at 1532.166 kb on - strand at 1532.175 kb on + strand at 1532.206 kb on + strand at 1532.272 kb on + strand at 1532.273 kb on - strand at 1532.273 kb on - strand at 1532.330 kb on - strand at 1532.340 kb on - strand at 1532.382 kb on - strand at 1532.401 kb on + strand at 1532.402 kb on - strand at 1532.455 kb on + strand at 1532.456 kb on - strand at 1532.805 kb on + strand, within IAI46_07225 at 1532.805 kb on + strand, within IAI46_07225 at 1532.850 kb on + strand, within IAI46_07225 at 1532.851 kb on - strand, within IAI46_07225 at 1532.851 kb on - strand, within IAI46_07225 at 1532.851 kb on - strand, within IAI46_07225 at 1532.851 kb on - strand, within IAI46_07225 at 1532.851 kb on - strand, within IAI46_07225 at 1532.908 kb on - strand, within IAI46_07225 at 1532.908 kb on - strand, within IAI46_07225 at 1532.908 kb on - strand, within IAI46_07225 at 1532.936 kb on - strand, within IAI46_07225 at 1532.991 kb on + strand, within IAI46_07225 at 1532.991 kb on + strand, within IAI46_07225 at 1532.991 kb on + strand, within IAI46_07225 at 1532.991 kb on + strand, within IAI46_07225 at 1532.992 kb on - strand, within IAI46_07225 at 1532.992 kb on - strand, within IAI46_07225 at 1532.992 kb on - strand, within IAI46_07225 at 1532.993 kb on + strand, within IAI46_07225 at 1532.994 kb on - strand, within IAI46_07225 at 1533.006 kb on + strand, within IAI46_07225 at 1533.035 kb on + strand, within IAI46_07225 at 1533.109 kb on - strand, within IAI46_07225 at 1533.109 kb on - strand, within IAI46_07225 at 1533.141 kb on - strand, within IAI46_07225
Per-strain Table
Position Strand Gene LocusTag Fraction Manganese (II) chloride tetrahydrate 50 uM, anaerobic remove 1,530,695 - IAI46_07215 0.79 -0.6 1,530,721 + IAI46_07215 0.80 -0.5 1,530,781 + IAI46_07215 0.81 +0.4 1,530,835 + IAI46_07215 0.83 +0.4 1,530,835 + IAI46_07215 0.83 -0.2 1,530,835 + IAI46_07215 0.83 -0.3 1,530,835 + IAI46_07215 0.83 +1.1 1,530,835 + IAI46_07215 0.83 -2.1 1,530,835 + IAI46_07215 0.83 +0.5 1,530,835 + IAI46_07215 0.83 -0.6 1,530,836 - IAI46_07215 0.83 +1.1 1,530,836 - IAI46_07215 0.83 -0.2 1,530,847 + IAI46_07215 0.83 +0.0 1,530,847 + IAI46_07215 0.83 -0.0 1,530,848 - IAI46_07215 0.83 +0.6 1,530,848 - IAI46_07215 0.83 -1.4 1,530,848 - IAI46_07215 0.83 -0.2 1,530,888 + IAI46_07215 0.84 -0.2 1,530,888 + IAI46_07215 0.84 -0.1 1,530,889 - IAI46_07215 0.84 -1.2 1,531,524 + +0.3 1,531,563 + -0.4 1,531,563 + -0.8 1,531,563 + -0.5 1,531,563 + -3.0 1,531,563 + +0.2 1,531,563 + -0.3 1,531,564 - -0.9 1,531,564 - -0.2 1,531,567 + +1.3 1,531,567 + -0.2 1,531,568 - +0.0 1,531,648 - IAI46_07220 0.20 +0.9 1,531,706 - IAI46_07220 0.28 -0.1 1,531,706 - IAI46_07220 0.28 +0.6 1,531,706 - IAI46_07220 0.28 +0.5 1,531,709 + IAI46_07220 0.28 -0.2 1,531,709 + IAI46_07220 0.28 +1.0 1,531,709 + IAI46_07220 0.28 -0.2 1,531,710 - IAI46_07220 0.29 +0.6 1,531,710 - IAI46_07220 0.29 -0.6 1,531,760 - IAI46_07220 0.36 -0.1 1,531,774 + IAI46_07220 0.38 +0.0 1,531,774 + IAI46_07220 0.38 -0.2 1,531,775 - IAI46_07220 0.38 +0.4 1,531,775 - IAI46_07220 0.38 -1.1 1,531,775 - IAI46_07220 0.38 -1.6 1,531,775 - IAI46_07220 0.38 -0.4 1,531,775 - IAI46_07220 0.38 +1.1 1,531,775 - IAI46_07220 0.38 +0.7 1,531,935 + IAI46_07220 0.61 +1.4 1,532,056 + IAI46_07220 0.78 +0.2 1,532,057 - IAI46_07220 0.78 +0.2 1,532,057 - IAI46_07220 0.78 +0.5 1,532,134 - IAI46_07220 0.89 -0.5 1,532,166 - -0.1 1,532,175 + +0.9 1,532,206 + -0.1 1,532,272 + +0.1 1,532,273 - -0.1 1,532,273 - -0.3 1,532,330 - -1.7 1,532,340 - -0.1 1,532,382 - +0.6 1,532,401 + +0.6 1,532,402 - +0.5 1,532,455 + -1.3 1,532,456 - +0.2 1,532,805 + IAI46_07225 0.33 -0.7 1,532,805 + IAI46_07225 0.33 +1.9 1,532,850 + IAI46_07225 0.37 +0.8 1,532,851 - IAI46_07225 0.37 +0.6 1,532,851 - IAI46_07225 0.37 +0.4 1,532,851 - IAI46_07225 0.37 +0.2 1,532,851 - IAI46_07225 0.37 -0.3 1,532,851 - IAI46_07225 0.37 -0.2 1,532,908 - IAI46_07225 0.41 -0.3 1,532,908 - IAI46_07225 0.41 +0.3 1,532,908 - IAI46_07225 0.41 +0.6 1,532,936 - IAI46_07225 0.44 +1.4 1,532,991 + IAI46_07225 0.48 +0.5 1,532,991 + IAI46_07225 0.48 +1.2 1,532,991 + IAI46_07225 0.48 +1.0 1,532,991 + IAI46_07225 0.48 -2.8 1,532,992 - IAI46_07225 0.48 -0.4 1,532,992 - IAI46_07225 0.48 +0.6 1,532,992 - IAI46_07225 0.48 +0.0 1,532,993 + IAI46_07225 0.48 +0.2 1,532,994 - IAI46_07225 0.48 -0.6 1,533,006 + IAI46_07225 0.49 -1.4 1,533,035 + IAI46_07225 0.51 +0.7 1,533,109 - IAI46_07225 0.57 +0.6 1,533,109 - IAI46_07225 0.57 +0.4 1,533,141 - IAI46_07225 0.60 -1.1
Or see this region's nucleotide sequence