Experiment: Manganese (II) chloride tetrahydrate 50 uM, anaerobic
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt IAI46_03530 and IAI46_03535 are separated by 85 nucleotides IAI46_03535 and IAI46_03540 overlap by 1 nucleotides IAI46_03540 and IAI46_03545 are separated by 70 nucleotides
IAI46_03530: IAI46_03530 - carboxylating nicotinate-nucleotide diphosphorylase, at 764,577 to 765,467
_03530
IAI46_03535: IAI46_03535 - 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD, at 765,553 to 766,149
_03535
IAI46_03540: IAI46_03540 - beta-lactamase regulator AmpE, at 766,149 to 767,003
_03540
IAI46_03545: IAI46_03545 - glutamate-1-semialdehyde 2,1-aminomutase, at 767,074 to 768,363
_03545
Position (kb) 765
766
767 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 764.578 kb on + strand at 764.579 kb on - strand at 764.579 kb on - strand at 764.579 kb on - strand at 764.639 kb on + strand at 764.640 kb on - strand at 764.640 kb on - strand at 764.640 kb on - strand at 764.640 kb on - strand at 764.642 kb on - strand at 764.774 kb on + strand, within IAI46_03530 at 764.774 kb on + strand, within IAI46_03530 at 764.774 kb on + strand, within IAI46_03530 at 764.774 kb on + strand, within IAI46_03530 at 764.775 kb on - strand, within IAI46_03530 at 764.775 kb on - strand, within IAI46_03530 at 764.901 kb on + strand, within IAI46_03530 at 764.971 kb on - strand, within IAI46_03530 at 764.977 kb on + strand, within IAI46_03530 at 765.079 kb on - strand, within IAI46_03530 at 765.182 kb on + strand, within IAI46_03530 at 765.183 kb on - strand, within IAI46_03530 at 765.395 kb on - strand at 765.402 kb on - strand at 765.451 kb on + strand at 765.451 kb on + strand at 765.511 kb on + strand at 765.511 kb on + strand at 765.511 kb on + strand at 765.511 kb on + strand at 765.511 kb on + strand at 765.512 kb on - strand at 765.512 kb on - strand at 765.512 kb on - strand at 765.512 kb on - strand at 765.512 kb on - strand at 765.512 kb on - strand at 765.531 kb on + strand at 765.531 kb on + strand at 765.531 kb on + strand at 765.532 kb on - strand at 765.532 kb on - strand at 765.561 kb on - strand at 765.657 kb on + strand, within IAI46_03535 at 765.691 kb on + strand, within IAI46_03535 at 765.691 kb on + strand, within IAI46_03535 at 765.692 kb on - strand, within IAI46_03535 at 765.692 kb on - strand, within IAI46_03535 at 765.692 kb on - strand, within IAI46_03535 at 765.692 kb on - strand, within IAI46_03535 at 765.715 kb on - strand, within IAI46_03535 at 765.761 kb on + strand, within IAI46_03535 at 765.761 kb on + strand, within IAI46_03535 at 765.812 kb on - strand, within IAI46_03535 at 765.876 kb on + strand, within IAI46_03535 at 765.941 kb on - strand, within IAI46_03535 at 765.941 kb on - strand, within IAI46_03535 at 766.018 kb on - strand, within IAI46_03535 at 766.018 kb on - strand, within IAI46_03535 at 766.035 kb on + strand, within IAI46_03535 at 766.036 kb on - strand, within IAI46_03535 at 766.070 kb on + strand, within IAI46_03535 at 766.071 kb on - strand, within IAI46_03535 at 766.072 kb on + strand, within IAI46_03535 at 766.072 kb on + strand, within IAI46_03535 at 766.113 kb on + strand at 766.113 kb on + strand at 766.113 kb on + strand at 766.114 kb on - strand at 766.161 kb on - strand at 766.169 kb on - strand at 766.169 kb on - strand at 766.193 kb on - strand at 766.240 kb on + strand, within IAI46_03540 at 766.241 kb on - strand, within IAI46_03540 at 766.241 kb on - strand, within IAI46_03540 at 766.241 kb on - strand, within IAI46_03540 at 766.282 kb on + strand, within IAI46_03540 at 766.283 kb on - strand, within IAI46_03540 at 766.351 kb on + strand, within IAI46_03540 at 766.351 kb on + strand, within IAI46_03540 at 766.351 kb on + strand, within IAI46_03540 at 766.413 kb on - strand, within IAI46_03540 at 766.428 kb on + strand, within IAI46_03540 at 766.429 kb on - strand, within IAI46_03540 at 766.429 kb on - strand, within IAI46_03540 at 766.565 kb on + strand, within IAI46_03540 at 766.642 kb on - strand, within IAI46_03540 at 766.683 kb on - strand, within IAI46_03540 at 766.770 kb on + strand, within IAI46_03540 at 766.956 kb on - strand at 766.956 kb on - strand at 766.956 kb on - strand at 766.987 kb on - strand at 767.001 kb on + strand at 767.002 kb on - strand at 767.075 kb on + strand at 767.076 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Manganese (II) chloride tetrahydrate 50 uM, anaerobic remove 764,578 + +0.2 764,579 - -1.1 764,579 - +0.9 764,579 - +0.2 764,639 + -0.6 764,640 - -0.8 764,640 - +0.7 764,640 - -0.1 764,640 - +0.3 764,642 - -0.8 764,774 + IAI46_03530 0.22 -0.6 764,774 + IAI46_03530 0.22 +1.1 764,774 + IAI46_03530 0.22 -0.0 764,774 + IAI46_03530 0.22 +0.4 764,775 - IAI46_03530 0.22 +0.1 764,775 - IAI46_03530 0.22 -0.1 764,901 + IAI46_03530 0.36 +0.1 764,971 - IAI46_03530 0.44 +0.2 764,977 + IAI46_03530 0.45 +0.2 765,079 - IAI46_03530 0.56 -2.9 765,182 + IAI46_03530 0.68 -1.3 765,183 - IAI46_03530 0.68 +1.3 765,395 - +0.9 765,402 - +0.3 765,451 + +0.2 765,451 + -0.8 765,511 + -0.4 765,511 + +0.2 765,511 + -0.5 765,511 + -0.1 765,511 + -0.2 765,512 - -0.0 765,512 - -0.3 765,512 - +0.2 765,512 - +0.9 765,512 - +0.2 765,512 - +0.1 765,531 + +0.4 765,531 + -1.1 765,531 + -1.8 765,532 - +0.0 765,532 - +0.7 765,561 - +0.2 765,657 + IAI46_03535 0.17 +1.3 765,691 + IAI46_03535 0.23 +0.7 765,691 + IAI46_03535 0.23 +1.7 765,692 - IAI46_03535 0.23 +0.1 765,692 - IAI46_03535 0.23 -0.2 765,692 - IAI46_03535 0.23 +0.2 765,692 - IAI46_03535 0.23 +1.0 765,715 - IAI46_03535 0.27 +1.2 765,761 + IAI46_03535 0.35 +0.1 765,761 + IAI46_03535 0.35 +0.6 765,812 - IAI46_03535 0.43 -1.8 765,876 + IAI46_03535 0.54 -1.9 765,941 - IAI46_03535 0.65 +1.3 765,941 - IAI46_03535 0.65 -0.7 766,018 - IAI46_03535 0.78 +0.7 766,018 - IAI46_03535 0.78 +0.8 766,035 + IAI46_03535 0.81 -0.8 766,036 - IAI46_03535 0.81 +0.5 766,070 + IAI46_03535 0.87 +0.0 766,071 - IAI46_03535 0.87 +0.6 766,072 + IAI46_03535 0.87 -1.1 766,072 + IAI46_03535 0.87 -0.8 766,113 + +0.4 766,113 + -1.1 766,113 + +0.2 766,114 - +1.2 766,161 - +1.1 766,169 - +0.1 766,169 - -0.1 766,193 - +0.9 766,240 + IAI46_03540 0.11 +0.5 766,241 - IAI46_03540 0.11 +0.5 766,241 - IAI46_03540 0.11 -0.3 766,241 - IAI46_03540 0.11 -0.7 766,282 + IAI46_03540 0.16 -0.4 766,283 - IAI46_03540 0.16 -0.1 766,351 + IAI46_03540 0.24 -0.0 766,351 + IAI46_03540 0.24 -0.3 766,351 + IAI46_03540 0.24 -0.5 766,413 - IAI46_03540 0.31 +0.2 766,428 + IAI46_03540 0.33 +0.6 766,429 - IAI46_03540 0.33 +1.0 766,429 - IAI46_03540 0.33 +0.1 766,565 + IAI46_03540 0.49 +0.1 766,642 - IAI46_03540 0.58 -0.9 766,683 - IAI46_03540 0.62 +0.3 766,770 + IAI46_03540 0.73 +1.5 766,956 - +0.5 766,956 - -0.2 766,956 - +1.1 766,987 - -0.2 767,001 + +0.2 767,002 - -0.0 767,075 + +0.8 767,076 - +0.1
Or see this region's nucleotide sequence