Experiment: D-Alanine (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ABIE41_RS15655 and ABIE41_RS15660 are separated by 174 nucleotides ABIE41_RS15660 and ABIE41_RS15665 are separated by 22 nucleotides ABIE41_RS15665 and ABIE41_RS15670 overlap by 1 nucleotides ABIE41_RS15670 and ABIE41_RS15675 are separated by 188 nucleotides
ABIE41_RS15655: ABIE41_RS15655 - TolC family outer membrane protein, at 3,205,248 to 3,206,645
_RS15655
ABIE41_RS15660: ABIE41_RS15660 - DUF2497 domain-containing protein, at 3,206,820 to 3,207,395
_RS15660
ABIE41_RS15665: ABIE41_RS15665 - hypothetical protein, at 3,207,418 to 3,207,648
_RS15665
ABIE41_RS15670: ABIE41_RS15670 - pyridoxamine 5'-phosphate oxidase family protein, at 3,207,648 to 3,208,133
_RS15670
ABIE41_RS15675: ABIE41_RS15675 - valine--tRNA ligase, at 3,208,322 to 3,211,141
_RS15675
Position (kb) 3206
3207
3208 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 3206.088 kb on + strand, within ABIE41_RS15655 at 3206.127 kb on + strand, within ABIE41_RS15655 at 3206.128 kb on - strand, within ABIE41_RS15655 at 3206.196 kb on + strand, within ABIE41_RS15655 at 3206.196 kb on + strand, within ABIE41_RS15655 at 3206.197 kb on - strand, within ABIE41_RS15655 at 3206.197 kb on - strand, within ABIE41_RS15655 at 3206.574 kb on + strand at 3206.574 kb on + strand at 3207.287 kb on + strand, within ABIE41_RS15660 at 3207.288 kb on - strand, within ABIE41_RS15660 at 3207.288 kb on - strand, within ABIE41_RS15660 at 3207.895 kb on + strand, within ABIE41_RS15670 at 3207.895 kb on + strand, within ABIE41_RS15670 at 3207.896 kb on - strand, within ABIE41_RS15670 at 3207.896 kb on - strand, within ABIE41_RS15670 at 3208.205 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction D-Alanine (C) remove 3,206,088 + ABIE41_RS15655 0.60 -1.5 3,206,127 + ABIE41_RS15655 0.63 +0.6 3,206,128 - ABIE41_RS15655 0.63 -0.1 3,206,196 + ABIE41_RS15655 0.68 -0.8 3,206,196 + ABIE41_RS15655 0.68 -3.2 3,206,197 - ABIE41_RS15655 0.68 +1.6 3,206,197 - ABIE41_RS15655 0.68 -0.7 3,206,574 + +1.5 3,206,574 + +0.2 3,207,287 + ABIE41_RS15660 0.81 -1.1 3,207,288 - ABIE41_RS15660 0.81 -0.1 3,207,288 - ABIE41_RS15660 0.81 -1.0 3,207,895 + ABIE41_RS15670 0.51 +0.2 3,207,895 + ABIE41_RS15670 0.51 +1.0 3,207,896 - ABIE41_RS15670 0.51 +1.9 3,207,896 - ABIE41_RS15670 0.51 +0.6 3,208,205 + -0.9
Or see this region's nucleotide sequence