Experiment: L-Phenylalanine (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ABID97_RS20490 and ABID97_RS20495 are separated by 15 nucleotides ABID97_RS20495 and ABID97_RS20500 are separated by 68 nucleotides ABID97_RS20500 and ABID97_RS20505 are separated by 117 nucleotides ABID97_RS20505 and ABID97_RS20510 are separated by 38 nucleotides ABID97_RS20510 and ABID97_RS20515 overlap by 7 nucleotides
ABID97_RS20490: ABID97_RS20490 - NADH-quinone oxidoreductase subunit B, at 4,230,681 to 4,231,160
_RS20490
ABID97_RS20495: ABID97_RS20495 - NADH-quinone oxidoreductase subunit A, at 4,231,176 to 4,231,535
_RS20495
ABID97_RS20500: ABID97_RS20500 - tRNA-Leu, at 4,231,604 to 4,231,688
_RS20500
ABID97_RS20505: ABID97_RS20505 - preprotein translocase subunit SecG, at 4,231,806 to 4,232,222
_RS20505
ABID97_RS20510: ABID97_RS20510 - triose-phosphate isomerase, at 4,232,261 to 4,233,046
_RS20510
ABID97_RS20515: ABID97_RS20515 - NAD(P)H-quinone oxidoreductase, at 4,233,040 to 4,234,038
_RS20515
Position (kb)
4231
4232
4233 Strain fitness (log2 ratio)
-1
0
1
2
3
4 at 4230.836 kb on - strand, within ABID97_RS20490 at 4231.715 kb on - strand at 4231.756 kb on - strand at 4231.999 kb on + strand, within ABID97_RS20505 at 4231.999 kb on + strand, within ABID97_RS20505 at 4232.237 kb on - strand at 4232.237 kb on - strand at 4232.237 kb on - strand at 4232.241 kb on - strand at 4232.241 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Phenylalanine (C) remove 4,230,836 - ABID97_RS20490 0.32 +3.5 4,231,715 - +3.2 4,231,756 - +1.2 4,231,999 + ABID97_RS20505 0.46 +4.5 4,231,999 + ABID97_RS20505 0.46 +4.4 4,232,237 - +1.0 4,232,237 - +0.8 4,232,237 - +0.6 4,232,241 - +0.7 4,232,241 - -0.3
Or see this region's nucleotide sequence