Experiment: D-Alanine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt livK and yhhK are separated by 423 nucleotides yhhK and livJ are separated by 187 nucleotides
b3458: livK - leucine transporter subunit (NCBI), at 3,594,474 to 3,595,583
livK
b3459: yhhK - hypothetical protein (NCBI), at 3,596,007 to 3,596,390
yhhK
b3460: livJ - leucine/isoleucine/valine transporter subunit (NCBI), at 3,596,578 to 3,597,681
livJ
Position (kb)
3596
3597 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 3595.035 kb on + strand, within livK at 3595.043 kb on - strand, within livK at 3595.053 kb on - strand, within livK at 3595.097 kb on + strand, within livK at 3595.109 kb on - strand, within livK at 3595.142 kb on + strand, within livK at 3595.199 kb on + strand, within livK at 3595.207 kb on - strand, within livK at 3595.207 kb on - strand, within livK at 3595.207 kb on - strand, within livK at 3595.264 kb on + strand, within livK at 3595.276 kb on - strand, within livK at 3595.343 kb on - strand, within livK at 3595.343 kb on - strand, within livK at 3595.354 kb on - strand, within livK at 3595.365 kb on + strand, within livK at 3595.427 kb on - strand, within livK at 3595.466 kb on + strand, within livK at 3595.466 kb on + strand, within livK at 3595.510 kb on - strand at 3595.517 kb on + strand at 3595.517 kb on + strand at 3595.549 kb on - strand at 3595.553 kb on - strand at 3595.553 kb on - strand at 3595.554 kb on + strand at 3595.637 kb on + strand at 3595.669 kb on + strand at 3595.968 kb on + strand at 3595.975 kb on + strand at 3596.005 kb on + strand at 3596.005 kb on - strand at 3596.005 kb on - strand at 3596.007 kb on + strand at 3596.011 kb on - strand at 3596.012 kb on - strand at 3596.013 kb on - strand at 3596.043 kb on + strand at 3596.048 kb on + strand, within yhhK at 3596.048 kb on + strand, within yhhK at 3596.056 kb on - strand, within yhhK at 3596.056 kb on - strand, within yhhK at 3596.061 kb on - strand, within yhhK at 3596.117 kb on + strand, within yhhK at 3596.128 kb on - strand, within yhhK at 3596.163 kb on - strand, within yhhK at 3596.163 kb on - strand, within yhhK at 3596.169 kb on + strand, within yhhK at 3596.209 kb on + strand, within yhhK at 3596.209 kb on + strand, within yhhK at 3596.251 kb on - strand, within yhhK at 3596.251 kb on - strand, within yhhK at 3596.267 kb on + strand, within yhhK at 3596.267 kb on + strand, within yhhK at 3596.288 kb on + strand, within yhhK at 3596.334 kb on + strand, within yhhK at 3596.420 kb on + strand at 3596.427 kb on + strand at 3596.427 kb on + strand at 3596.500 kb on + strand at 3596.588 kb on - strand at 3596.657 kb on + strand at 3596.737 kb on + strand, within livJ at 3596.906 kb on + strand, within livJ at 3596.914 kb on - strand, within livJ at 3596.914 kb on - strand, within livJ at 3596.994 kb on - strand, within livJ at 3596.994 kb on - strand, within livJ at 3597.006 kb on + strand, within livJ at 3597.133 kb on + strand, within livJ at 3597.141 kb on - strand, within livJ at 3597.141 kb on - strand, within livJ at 3597.141 kb on - strand, within livJ at 3597.141 kb on - strand, within livJ at 3597.148 kb on + strand, within livJ at 3597.154 kb on + strand, within livJ at 3597.154 kb on + strand, within livJ at 3597.156 kb on - strand, within livJ at 3597.161 kb on + strand, within livJ at 3597.188 kb on + strand, within livJ at 3597.188 kb on + strand, within livJ at 3597.240 kb on + strand, within livJ at 3597.240 kb on + strand, within livJ at 3597.240 kb on - strand, within livJ at 3597.250 kb on + strand, within livJ at 3597.250 kb on + strand, within livJ at 3597.251 kb on - strand, within livJ at 3597.251 kb on - strand, within livJ at 3597.251 kb on - strand, within livJ at 3597.276 kb on + strand, within livJ at 3597.312 kb on + strand, within livJ at 3597.312 kb on + strand, within livJ at 3597.320 kb on - strand, within livJ at 3597.377 kb on + strand, within livJ at 3597.377 kb on + strand, within livJ at 3597.385 kb on - strand, within livJ at 3597.385 kb on - strand, within livJ at 3597.385 kb on - strand, within livJ at 3597.385 kb on - strand, within livJ
Per-strain Table
Position Strand Gene LocusTag Fraction D-Alanine (N) remove 3,595,035 + livK b3458 0.51 -1.1 3,595,043 - livK b3458 0.51 +0.1 3,595,053 - livK b3458 0.52 -0.1 3,595,097 + livK b3458 0.56 -0.0 3,595,109 - livK b3458 0.57 -1.2 3,595,142 + livK b3458 0.60 +0.2 3,595,199 + livK b3458 0.65 +0.6 3,595,207 - livK b3458 0.66 -0.3 3,595,207 - livK b3458 0.66 +0.3 3,595,207 - livK b3458 0.66 +0.1 3,595,264 + livK b3458 0.71 +0.1 3,595,276 - livK b3458 0.72 +0.1 3,595,343 - livK b3458 0.78 -0.2 3,595,343 - livK b3458 0.78 +1.4 3,595,354 - livK b3458 0.79 -0.5 3,595,365 + livK b3458 0.80 +0.5 3,595,427 - livK b3458 0.86 -0.7 3,595,466 + livK b3458 0.89 +0.1 3,595,466 + livK b3458 0.89 +0.3 3,595,510 - +0.6 3,595,517 + -0.4 3,595,517 + +0.3 3,595,549 - +0.1 3,595,553 - -1.0 3,595,553 - -1.5 3,595,554 + +0.2 3,595,637 + -0.2 3,595,669 + +0.0 3,595,968 + -1.1 3,595,975 + -0.3 3,596,005 + +1.1 3,596,005 - +0.2 3,596,005 - +0.7 3,596,007 + -0.2 3,596,011 - +0.3 3,596,012 - +0.2 3,596,013 - -0.1 3,596,043 + -1.0 3,596,048 + yhhK b3459 0.11 +0.1 3,596,048 + yhhK b3459 0.11 -0.4 3,596,056 - yhhK b3459 0.13 -2.8 3,596,056 - yhhK b3459 0.13 -0.8 3,596,061 - yhhK b3459 0.14 -1.3 3,596,117 + yhhK b3459 0.29 -3.5 3,596,128 - yhhK b3459 0.32 -2.8 3,596,163 - yhhK b3459 0.41 -1.3 3,596,163 - yhhK b3459 0.41 -1.6 3,596,169 + yhhK b3459 0.42 -2.3 3,596,209 + yhhK b3459 0.53 -0.6 3,596,209 + yhhK b3459 0.53 -0.9 3,596,251 - yhhK b3459 0.64 -1.9 3,596,251 - yhhK b3459 0.64 -0.0 3,596,267 + yhhK b3459 0.68 -1.2 3,596,267 + yhhK b3459 0.68 -1.0 3,596,288 + yhhK b3459 0.73 -0.4 3,596,334 + yhhK b3459 0.85 -2.6 3,596,420 + -0.2 3,596,427 + -0.0 3,596,427 + -0.2 3,596,500 + -0.5 3,596,588 - +0.8 3,596,657 + +0.7 3,596,737 + livJ b3460 0.14 -0.7 3,596,906 + livJ b3460 0.30 +0.3 3,596,914 - livJ b3460 0.30 +0.0 3,596,914 - livJ b3460 0.30 -0.7 3,596,994 - livJ b3460 0.38 +0.0 3,596,994 - livJ b3460 0.38 +0.9 3,597,006 + livJ b3460 0.39 -0.8 3,597,133 + livJ b3460 0.50 -0.3 3,597,141 - livJ b3460 0.51 -0.1 3,597,141 - livJ b3460 0.51 -0.6 3,597,141 - livJ b3460 0.51 +0.3 3,597,141 - livJ b3460 0.51 -0.3 3,597,148 + livJ b3460 0.52 +0.1 3,597,154 + livJ b3460 0.52 -0.9 3,597,154 + livJ b3460 0.52 +0.4 3,597,156 - livJ b3460 0.52 -0.3 3,597,161 + livJ b3460 0.53 -0.1 3,597,188 + livJ b3460 0.55 +0.1 3,597,188 + livJ b3460 0.55 -1.1 3,597,240 + livJ b3460 0.60 +0.0 3,597,240 + livJ b3460 0.60 +0.2 3,597,240 - livJ b3460 0.60 +0.4 3,597,250 + livJ b3460 0.61 -1.0 3,597,250 + livJ b3460 0.61 -0.3 3,597,251 - livJ b3460 0.61 -1.0 3,597,251 - livJ b3460 0.61 -0.1 3,597,251 - livJ b3460 0.61 -0.1 3,597,276 + livJ b3460 0.63 -0.0 3,597,312 + livJ b3460 0.66 +0.7 3,597,312 + livJ b3460 0.66 -0.7 3,597,320 - livJ b3460 0.67 -0.8 3,597,377 + livJ b3460 0.72 +0.6 3,597,377 + livJ b3460 0.72 +0.1 3,597,385 - livJ b3460 0.73 -0.2 3,597,385 - livJ b3460 0.73 -0.4 3,597,385 - livJ b3460 0.73 -0.1 3,597,385 - livJ b3460 0.73 -0.2
Or see this region's nucleotide sequence