Experiment: D-Alanine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt mdlB and glnK are separated by 180 nucleotides glnK and amtB are separated by 29 nucleotides amtB and tesB are separated by 48 nucleotides
b0449: mdlB - fused predicted multidrug transporter subunits of ABC superfamily: ATP-binding components (NCBI), at 469,860 to 471,641
mdlB
b0450: glnK - nitrogen assimilation regulatory protein for GlnL, GlnE, and AmtB (NCBI), at 471,822 to 472,160
glnK
b0451: amtB - ammonium transporter (NCBI), at 472,190 to 473,476
amtB
b0452: tesB - acyl-CoA thioesterase II (NCBI), at 473,525 to 474,385
tesB
Position (kb) 472
473
474 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 471.224 kb on + strand, within mdlB at 471.410 kb on - strand, within mdlB at 471.433 kb on + strand, within mdlB at 471.443 kb on + strand, within mdlB at 471.447 kb on - strand, within mdlB at 471.510 kb on - strand at 471.522 kb on - strand at 471.549 kb on + strand at 471.551 kb on + strand at 471.551 kb on + strand at 471.679 kb on + strand at 471.692 kb on - strand at 471.699 kb on - strand at 471.866 kb on + strand, within glnK at 471.866 kb on + strand, within glnK at 471.884 kb on - strand, within glnK at 472.069 kb on - strand, within glnK at 472.140 kb on + strand at 472.180 kb on - strand at 472.254 kb on - strand at 472.257 kb on + strand at 472.345 kb on + strand, within amtB at 472.345 kb on + strand, within amtB at 472.415 kb on - strand, within amtB at 472.417 kb on + strand, within amtB at 472.430 kb on - strand, within amtB at 472.444 kb on - strand, within amtB at 472.546 kb on + strand, within amtB at 472.669 kb on + strand, within amtB at 472.727 kb on + strand, within amtB at 472.751 kb on + strand, within amtB at 472.828 kb on + strand, within amtB at 472.876 kb on + strand, within amtB at 472.876 kb on + strand, within amtB at 473.140 kb on + strand, within amtB at 473.280 kb on + strand, within amtB at 473.295 kb on - strand, within amtB at 473.420 kb on - strand at 473.420 kb on - strand at 473.549 kb on + strand at 473.549 kb on + strand at 473.549 kb on + strand at 473.575 kb on - strand at 473.575 kb on - strand at 473.586 kb on + strand at 473.644 kb on - strand, within tesB at 473.651 kb on - strand, within tesB at 473.651 kb on - strand, within tesB at 473.651 kb on - strand, within tesB at 473.651 kb on - strand, within tesB at 473.855 kb on + strand, within tesB at 473.855 kb on + strand, within tesB at 473.901 kb on - strand, within tesB at 474.002 kb on + strand, within tesB at 474.055 kb on - strand, within tesB at 474.065 kb on - strand, within tesB at 474.105 kb on + strand, within tesB at 474.135 kb on - strand, within tesB at 474.135 kb on - strand, within tesB at 474.143 kb on - strand, within tesB at 474.145 kb on - strand, within tesB at 474.169 kb on + strand, within tesB at 474.195 kb on + strand, within tesB at 474.303 kb on - strand at 474.390 kb on + strand at 474.403 kb on - strand at 474.464 kb on + strand at 474.464 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction D-Alanine (N) remove 471,224 + mdlB b0449 0.77 -0.2 471,410 - mdlB b0449 0.87 -0.7 471,433 + mdlB b0449 0.88 +0.6 471,443 + mdlB b0449 0.89 -0.7 471,447 - mdlB b0449 0.89 -1.0 471,510 - -0.3 471,522 - -0.2 471,549 + +0.9 471,551 + +0.2 471,551 + -0.1 471,679 + -1.4 471,692 - -2.0 471,699 - -0.4 471,866 + glnK b0450 0.13 -0.2 471,866 + glnK b0450 0.13 +1.0 471,884 - glnK b0450 0.18 -3.5 472,069 - glnK b0450 0.73 -3.4 472,140 + -0.1 472,180 - -1.4 472,254 - -2.5 472,257 + -3.2 472,345 + amtB b0451 0.12 -1.8 472,345 + amtB b0451 0.12 -2.4 472,415 - amtB b0451 0.17 -2.6 472,417 + amtB b0451 0.18 -2.5 472,430 - amtB b0451 0.19 -1.5 472,444 - amtB b0451 0.20 -1.8 472,546 + amtB b0451 0.28 -2.9 472,669 + amtB b0451 0.37 -1.3 472,727 + amtB b0451 0.42 -3.4 472,751 + amtB b0451 0.44 -3.2 472,828 + amtB b0451 0.50 -0.7 472,876 + amtB b0451 0.53 -2.4 472,876 + amtB b0451 0.53 -2.3 473,140 + amtB b0451 0.74 -2.7 473,280 + amtB b0451 0.85 -3.0 473,295 - amtB b0451 0.86 -2.4 473,420 - -2.3 473,420 - -1.9 473,549 + +0.1 473,549 + -0.1 473,549 + -0.5 473,575 - +0.5 473,575 - +0.0 473,586 + -0.5 473,644 - tesB b0452 0.14 +1.4 473,651 - tesB b0452 0.15 +0.3 473,651 - tesB b0452 0.15 +0.0 473,651 - tesB b0452 0.15 +0.5 473,651 - tesB b0452 0.15 -0.9 473,855 + tesB b0452 0.38 -0.2 473,855 + tesB b0452 0.38 +0.0 473,901 - tesB b0452 0.44 -0.4 474,002 + tesB b0452 0.55 -0.6 474,055 - tesB b0452 0.62 +0.8 474,065 - tesB b0452 0.63 -0.5 474,105 + tesB b0452 0.67 -0.4 474,135 - tesB b0452 0.71 +0.4 474,135 - tesB b0452 0.71 +0.3 474,143 - tesB b0452 0.72 -0.1 474,145 - tesB b0452 0.72 +0.2 474,169 + tesB b0452 0.75 +0.3 474,195 + tesB b0452 0.78 +0.2 474,303 - +0.9 474,390 + -0.4 474,403 - -0.2 474,464 + +0.2 474,464 + -0.4
Or see this region's nucleotide sequence