Experiment: Cobalt chloride 60 uM, anaerobic
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt IAI46_20970 and IAI46_20975 are separated by 1 nucleotides IAI46_20975 and IAI46_20980 overlap by 7 nucleotides IAI46_20980 and IAI46_20985 are separated by 175 nucleotides
IAI46_20970: IAI46_20970 - 3-keto-L-gulonate-6-phosphate decarboxylase UlaD, at 4,439,008 to 4,439,664
_20970
IAI46_20975: IAI46_20975 - L-ribulose-5-phosphate 3-epimerase, at 4,439,666 to 4,440,526
_20975
IAI46_20980: IAI46_20980 - L-ribulose-5-phosphate 4-epimerase, at 4,440,520 to 4,441,221
_20980
IAI46_20985: IAI46_20985 - RluA family pseudouridine synthase, at 4,441,397 to 4,442,095
_20985
Position (kb)
4440
4441
4442 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 4440.026 kb on + strand, within IAI46_20975 at 4440.071 kb on - strand, within IAI46_20975 at 4440.071 kb on - strand, within IAI46_20975 at 4440.143 kb on - strand, within IAI46_20975 at 4440.305 kb on + strand, within IAI46_20975 at 4440.305 kb on + strand, within IAI46_20975 at 4440.306 kb on - strand, within IAI46_20975 at 4440.403 kb on + strand, within IAI46_20975 at 4440.403 kb on + strand, within IAI46_20975 at 4440.403 kb on + strand, within IAI46_20975 at 4440.403 kb on + strand, within IAI46_20975 at 4440.403 kb on + strand, within IAI46_20975 at 4440.404 kb on - strand, within IAI46_20975 at 4440.404 kb on - strand, within IAI46_20975 at 4440.404 kb on - strand, within IAI46_20975 at 4440.407 kb on + strand, within IAI46_20975 at 4440.524 kb on + strand at 4440.524 kb on + strand at 4440.541 kb on - strand at 4440.566 kb on + strand at 4440.566 kb on + strand at 4440.566 kb on + strand at 4440.566 kb on + strand at 4440.567 kb on - strand at 4440.668 kb on - strand, within IAI46_20980 at 4440.776 kb on - strand, within IAI46_20980 at 4440.829 kb on - strand, within IAI46_20980 at 4440.886 kb on + strand, within IAI46_20980 at 4440.893 kb on - strand, within IAI46_20980 at 4440.893 kb on - strand, within IAI46_20980 at 4440.893 kb on - strand, within IAI46_20980 at 4440.946 kb on + strand, within IAI46_20980 at 4440.946 kb on + strand, within IAI46_20980 at 4440.946 kb on + strand, within IAI46_20980 at 4440.946 kb on + strand, within IAI46_20980 at 4441.022 kb on + strand, within IAI46_20980 at 4441.108 kb on + strand, within IAI46_20980 at 4441.180 kb on + strand at 4441.181 kb on - strand at 4441.181 kb on - strand at 4441.208 kb on - strand at 4441.210 kb on + strand at 4441.211 kb on - strand at 4441.211 kb on - strand at 4441.242 kb on - strand at 4441.363 kb on - strand at 4441.402 kb on + strand at 4441.402 kb on + strand at 4441.402 kb on + strand at 4441.403 kb on - strand at 4441.403 kb on - strand at 4441.447 kb on - strand at 4441.447 kb on - strand at 4441.457 kb on + strand at 4441.458 kb on - strand at 4441.487 kb on - strand, within IAI46_20985 at 4441.504 kb on - strand, within IAI46_20985 at 4441.542 kb on + strand, within IAI46_20985 at 4441.543 kb on - strand, within IAI46_20985 at 4441.563 kb on + strand, within IAI46_20985 at 4441.563 kb on + strand, within IAI46_20985 at 4441.564 kb on - strand, within IAI46_20985 at 4441.576 kb on + strand, within IAI46_20985 at 4441.576 kb on + strand, within IAI46_20985 at 4441.577 kb on - strand, within IAI46_20985 at 4441.577 kb on - strand, within IAI46_20985 at 4441.631 kb on - strand, within IAI46_20985 at 4441.633 kb on + strand, within IAI46_20985 at 4441.634 kb on - strand, within IAI46_20985 at 4441.691 kb on + strand, within IAI46_20985 at 4441.691 kb on + strand, within IAI46_20985 at 4441.692 kb on - strand, within IAI46_20985 at 4441.737 kb on + strand, within IAI46_20985 at 4441.737 kb on + strand, within IAI46_20985 at 4441.737 kb on + strand, within IAI46_20985 at 4441.738 kb on - strand, within IAI46_20985 at 4441.738 kb on - strand, within IAI46_20985 at 4441.738 kb on - strand, within IAI46_20985 at 4441.738 kb on - strand, within IAI46_20985 at 4441.884 kb on + strand, within IAI46_20985 at 4441.891 kb on - strand, within IAI46_20985 at 4441.891 kb on - strand, within IAI46_20985 at 4441.925 kb on - strand, within IAI46_20985 at 4441.951 kb on + strand, within IAI46_20985 at 4441.952 kb on - strand, within IAI46_20985 at 4441.952 kb on - strand, within IAI46_20985 at 4441.971 kb on - strand, within IAI46_20985 at 4442.104 kb on + strand at 4442.174 kb on + strand at 4442.174 kb on + strand at 4442.175 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Cobalt chloride 60 uM, anaerobic remove 4,440,026 + IAI46_20975 0.42 +0.2 4,440,071 - IAI46_20975 0.47 -1.3 4,440,071 - IAI46_20975 0.47 +0.0 4,440,143 - IAI46_20975 0.55 -0.4 4,440,305 + IAI46_20975 0.74 -0.3 4,440,305 + IAI46_20975 0.74 -2.0 4,440,306 - IAI46_20975 0.74 +0.3 4,440,403 + IAI46_20975 0.86 -0.1 4,440,403 + IAI46_20975 0.86 -1.4 4,440,403 + IAI46_20975 0.86 +0.3 4,440,403 + IAI46_20975 0.86 -0.4 4,440,403 + IAI46_20975 0.86 +0.1 4,440,404 - IAI46_20975 0.86 +1.0 4,440,404 - IAI46_20975 0.86 +0.1 4,440,404 - IAI46_20975 0.86 +0.7 4,440,407 + IAI46_20975 0.86 +0.6 4,440,524 + +0.4 4,440,524 + +1.0 4,440,541 - +0.3 4,440,566 + -0.1 4,440,566 + +0.4 4,440,566 + +0.6 4,440,566 + +1.1 4,440,567 - +2.3 4,440,668 - IAI46_20980 0.21 +0.2 4,440,776 - IAI46_20980 0.36 +0.0 4,440,829 - IAI46_20980 0.44 +0.3 4,440,886 + IAI46_20980 0.52 -0.9 4,440,893 - IAI46_20980 0.53 +0.2 4,440,893 - IAI46_20980 0.53 -0.1 4,440,893 - IAI46_20980 0.53 +0.6 4,440,946 + IAI46_20980 0.61 -0.2 4,440,946 + IAI46_20980 0.61 -0.0 4,440,946 + IAI46_20980 0.61 -0.0 4,440,946 + IAI46_20980 0.61 -0.9 4,441,022 + IAI46_20980 0.72 -0.5 4,441,108 + IAI46_20980 0.84 +0.1 4,441,180 + +0.5 4,441,181 - -0.0 4,441,181 - +0.3 4,441,208 - +0.6 4,441,210 + -0.2 4,441,211 - -0.1 4,441,211 - +0.4 4,441,242 - -0.9 4,441,363 - -0.8 4,441,402 + +0.9 4,441,402 + -0.3 4,441,402 + +0.5 4,441,403 - +0.0 4,441,403 - -0.2 4,441,447 - -0.0 4,441,447 - +0.1 4,441,457 + +0.6 4,441,458 - +0.5 4,441,487 - IAI46_20985 0.13 -0.1 4,441,504 - IAI46_20985 0.15 +0.7 4,441,542 + IAI46_20985 0.21 +0.7 4,441,543 - IAI46_20985 0.21 -1.2 4,441,563 + IAI46_20985 0.24 +0.1 4,441,563 + IAI46_20985 0.24 -1.1 4,441,564 - IAI46_20985 0.24 -0.1 4,441,576 + IAI46_20985 0.26 -0.0 4,441,576 + IAI46_20985 0.26 -0.7 4,441,577 - IAI46_20985 0.26 +0.0 4,441,577 - IAI46_20985 0.26 -0.8 4,441,631 - IAI46_20985 0.33 +0.4 4,441,633 + IAI46_20985 0.34 +0.6 4,441,634 - IAI46_20985 0.34 +0.4 4,441,691 + IAI46_20985 0.42 +0.1 4,441,691 + IAI46_20985 0.42 -0.1 4,441,692 - IAI46_20985 0.42 -0.2 4,441,737 + IAI46_20985 0.49 -0.2 4,441,737 + IAI46_20985 0.49 +1.1 4,441,737 + IAI46_20985 0.49 +0.5 4,441,738 - IAI46_20985 0.49 -0.6 4,441,738 - IAI46_20985 0.49 -0.2 4,441,738 - IAI46_20985 0.49 +0.5 4,441,738 - IAI46_20985 0.49 -1.0 4,441,884 + IAI46_20985 0.70 -0.3 4,441,891 - IAI46_20985 0.71 +0.6 4,441,891 - IAI46_20985 0.71 -0.8 4,441,925 - IAI46_20985 0.76 -0.4 4,441,951 + IAI46_20985 0.79 -0.3 4,441,952 - IAI46_20985 0.79 +0.2 4,441,952 - IAI46_20985 0.79 +0.1 4,441,971 - IAI46_20985 0.82 -1.1 4,442,104 + +0.0 4,442,174 + +0.2 4,442,174 + -0.5 4,442,175 - -1.1
Or see this region's nucleotide sequence