Experiment: Cobalt chloride 60 uM, anaerobic
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt IAI46_20350 and IAI46_20355 are separated by 23 nucleotides IAI46_20355 and IAI46_20360 overlap by 10 nucleotides IAI46_20360 and IAI46_20365 are separated by 301 nucleotides
IAI46_20350: IAI46_20350 - cysteine desulfurase CsdA, at 4,299,728 to 4,300,933
_20350
IAI46_20355: IAI46_20355 - cysteine desulfurase sulfur acceptor subunit CsdE, at 4,300,957 to 4,301,391
_20355
IAI46_20360: IAI46_20360 - tRNA cyclic N6-threonylcarbamoyladenosine(37) synthase TcdA, at 4,301,382 to 4,302,188
_20360
IAI46_20365: IAI46_20365 - murein transglycosylase A, at 4,302,490 to 4,303,632
_20365
Position (kb)
4301
4302
4303 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 4300.422 kb on + strand, within IAI46_20350 at 4300.422 kb on + strand, within IAI46_20350 at 4300.423 kb on - strand, within IAI46_20350 at 4300.423 kb on - strand, within IAI46_20350 at 4300.423 kb on - strand, within IAI46_20350 at 4300.424 kb on + strand, within IAI46_20350 at 4300.425 kb on - strand, within IAI46_20350 at 4300.625 kb on + strand, within IAI46_20350 at 4300.625 kb on + strand, within IAI46_20350 at 4300.862 kb on + strand at 4300.863 kb on - strand at 4300.863 kb on - strand at 4300.863 kb on - strand at 4300.864 kb on + strand at 4300.864 kb on + strand at 4300.864 kb on + strand at 4300.864 kb on + strand at 4300.864 kb on + strand at 4300.865 kb on - strand at 4300.865 kb on - strand at 4300.865 kb on - strand at 4300.956 kb on + strand at 4300.956 kb on + strand at 4300.956 kb on + strand at 4300.962 kb on + strand at 4301.004 kb on - strand, within IAI46_20355 at 4301.044 kb on + strand, within IAI46_20355 at 4301.058 kb on + strand, within IAI46_20355 at 4301.058 kb on + strand, within IAI46_20355 at 4301.059 kb on - strand, within IAI46_20355 at 4301.059 kb on - strand, within IAI46_20355 at 4301.059 kb on - strand, within IAI46_20355 at 4301.059 kb on - strand, within IAI46_20355 at 4301.202 kb on + strand, within IAI46_20355 at 4301.202 kb on + strand, within IAI46_20355 at 4301.241 kb on - strand, within IAI46_20355 at 4301.384 kb on - strand at 4301.712 kb on + strand, within IAI46_20360 at 4301.748 kb on + strand, within IAI46_20360 at 4301.748 kb on + strand, within IAI46_20360 at 4301.748 kb on + strand, within IAI46_20360 at 4301.748 kb on + strand, within IAI46_20360 at 4301.749 kb on - strand, within IAI46_20360 at 4301.756 kb on - strand, within IAI46_20360 at 4301.756 kb on - strand, within IAI46_20360 at 4301.768 kb on - strand, within IAI46_20360 at 4301.768 kb on - strand, within IAI46_20360 at 4301.819 kb on - strand, within IAI46_20360 at 4301.862 kb on - strand, within IAI46_20360 at 4302.006 kb on - strand, within IAI46_20360 at 4302.008 kb on - strand, within IAI46_20360 at 4302.017 kb on + strand, within IAI46_20360 at 4302.018 kb on - strand, within IAI46_20360 at 4302.018 kb on - strand, within IAI46_20360 at 4302.130 kb on + strand at 4302.130 kb on + strand at 4302.131 kb on - strand at 4302.131 kb on - strand at 4302.163 kb on + strand at 4302.163 kb on + strand at 4302.164 kb on - strand at 4302.164 kb on - strand at 4302.164 kb on - strand at 4302.175 kb on + strand at 4302.197 kb on - strand at 4302.205 kb on - strand at 4302.219 kb on + strand at 4302.219 kb on + strand at 4302.220 kb on - strand at 4302.220 kb on - strand at 4302.362 kb on + strand at 4302.362 kb on + strand at 4302.362 kb on + strand at 4302.362 kb on + strand at 4302.362 kb on + strand at 4302.362 kb on + strand at 4302.363 kb on - strand at 4302.364 kb on + strand at 4302.364 kb on + strand at 4302.364 kb on + strand at 4302.365 kb on - strand at 4302.365 kb on - strand at 4302.556 kb on + strand at 4302.599 kb on + strand at 4302.600 kb on - strand at 4302.600 kb on - strand at 4302.600 kb on - strand at 4302.600 kb on - strand at 4302.600 kb on - strand at 4302.600 kb on - strand at 4302.603 kb on - strand at 4302.641 kb on + strand, within IAI46_20365 at 4302.641 kb on + strand, within IAI46_20365 at 4302.641 kb on + strand, within IAI46_20365 at 4302.641 kb on + strand, within IAI46_20365 at 4302.642 kb on - strand, within IAI46_20365 at 4302.642 kb on - strand, within IAI46_20365 at 4302.642 kb on - strand, within IAI46_20365 at 4302.642 kb on - strand, within IAI46_20365 at 4302.642 kb on - strand, within IAI46_20365 at 4302.954 kb on + strand, within IAI46_20365 at 4302.954 kb on + strand, within IAI46_20365 at 4302.955 kb on - strand, within IAI46_20365 at 4302.955 kb on - strand, within IAI46_20365 at 4302.955 kb on - strand, within IAI46_20365 at 4302.955 kb on - strand, within IAI46_20365 at 4303.064 kb on + strand, within IAI46_20365 at 4303.073 kb on + strand, within IAI46_20365 at 4303.074 kb on - strand, within IAI46_20365 at 4303.074 kb on - strand, within IAI46_20365 at 4303.098 kb on + strand, within IAI46_20365 at 4303.099 kb on - strand, within IAI46_20365 at 4303.125 kb on - strand, within IAI46_20365 at 4303.125 kb on - strand, within IAI46_20365 at 4303.134 kb on + strand, within IAI46_20365 at 4303.135 kb on - strand, within IAI46_20365 at 4303.137 kb on - strand, within IAI46_20365 at 4303.160 kb on + strand, within IAI46_20365
Per-strain Table
Position Strand Gene LocusTag Fraction Cobalt chloride 60 uM, anaerobic remove 4,300,422 + IAI46_20350 0.58 -0.1 4,300,422 + IAI46_20350 0.58 +0.5 4,300,423 - IAI46_20350 0.58 +0.7 4,300,423 - IAI46_20350 0.58 -0.2 4,300,423 - IAI46_20350 0.58 -0.3 4,300,424 + IAI46_20350 0.58 -0.1 4,300,425 - IAI46_20350 0.58 +0.3 4,300,625 + IAI46_20350 0.74 +0.3 4,300,625 + IAI46_20350 0.74 +0.4 4,300,862 + +0.2 4,300,863 - +0.9 4,300,863 - +0.5 4,300,863 - -1.0 4,300,864 + +0.3 4,300,864 + -1.1 4,300,864 + +0.7 4,300,864 + +1.1 4,300,864 + +0.0 4,300,865 - -0.6 4,300,865 - -0.8 4,300,865 - -0.4 4,300,956 + +0.6 4,300,956 + -0.0 4,300,956 + +0.4 4,300,962 + -0.2 4,301,004 - IAI46_20355 0.11 -0.1 4,301,044 + IAI46_20355 0.20 +0.3 4,301,058 + IAI46_20355 0.23 -0.2 4,301,058 + IAI46_20355 0.23 -0.5 4,301,059 - IAI46_20355 0.23 -0.3 4,301,059 - IAI46_20355 0.23 +1.5 4,301,059 - IAI46_20355 0.23 +0.3 4,301,059 - IAI46_20355 0.23 +0.5 4,301,202 + IAI46_20355 0.56 -1.2 4,301,202 + IAI46_20355 0.56 +2.6 4,301,241 - IAI46_20355 0.65 +0.5 4,301,384 - +0.1 4,301,712 + IAI46_20360 0.41 -1.2 4,301,748 + IAI46_20360 0.45 +0.3 4,301,748 + IAI46_20360 0.45 -0.1 4,301,748 + IAI46_20360 0.45 -0.2 4,301,748 + IAI46_20360 0.45 +0.0 4,301,749 - IAI46_20360 0.45 -0.6 4,301,756 - IAI46_20360 0.46 -0.9 4,301,756 - IAI46_20360 0.46 -0.5 4,301,768 - IAI46_20360 0.48 +0.4 4,301,768 - IAI46_20360 0.48 +0.1 4,301,819 - IAI46_20360 0.54 +0.6 4,301,862 - IAI46_20360 0.59 -0.4 4,302,006 - IAI46_20360 0.77 -0.4 4,302,008 - IAI46_20360 0.78 +0.8 4,302,017 + IAI46_20360 0.79 +0.5 4,302,018 - IAI46_20360 0.79 +0.3 4,302,018 - IAI46_20360 0.79 -1.0 4,302,130 + +0.7 4,302,130 + +0.5 4,302,131 - -1.1 4,302,131 - -1.3 4,302,163 + -0.3 4,302,163 + +0.2 4,302,164 - +0.1 4,302,164 - -1.3 4,302,164 - +0.3 4,302,175 + +0.2 4,302,197 - -0.2 4,302,205 - +0.1 4,302,219 + +1.1 4,302,219 + -0.6 4,302,220 - +1.2 4,302,220 - +0.2 4,302,362 + +0.1 4,302,362 + -0.3 4,302,362 + -0.4 4,302,362 + +0.5 4,302,362 + -0.8 4,302,362 + -1.2 4,302,363 - -0.1 4,302,364 + +0.4 4,302,364 + +0.1 4,302,364 + +0.7 4,302,365 - +0.0 4,302,365 - +0.1 4,302,556 + -0.9 4,302,599 + +0.1 4,302,600 - +0.3 4,302,600 - +0.3 4,302,600 - -0.2 4,302,600 - +0.5 4,302,600 - -2.6 4,302,600 - +0.2 4,302,603 - -0.4 4,302,641 + IAI46_20365 0.13 +0.1 4,302,641 + IAI46_20365 0.13 -1.1 4,302,641 + IAI46_20365 0.13 +0.5 4,302,641 + IAI46_20365 0.13 +1.5 4,302,642 - IAI46_20365 0.13 -1.1 4,302,642 - IAI46_20365 0.13 -0.5 4,302,642 - IAI46_20365 0.13 +0.1 4,302,642 - IAI46_20365 0.13 -0.2 4,302,642 - IAI46_20365 0.13 +0.2 4,302,954 + IAI46_20365 0.41 +0.0 4,302,954 + IAI46_20365 0.41 +1.7 4,302,955 - IAI46_20365 0.41 +0.5 4,302,955 - IAI46_20365 0.41 -0.1 4,302,955 - IAI46_20365 0.41 -0.3 4,302,955 - IAI46_20365 0.41 -0.0 4,303,064 + IAI46_20365 0.50 +0.7 4,303,073 + IAI46_20365 0.51 -0.4 4,303,074 - IAI46_20365 0.51 +0.2 4,303,074 - IAI46_20365 0.51 -0.3 4,303,098 + IAI46_20365 0.53 -0.8 4,303,099 - IAI46_20365 0.53 -1.1 4,303,125 - IAI46_20365 0.56 +0.6 4,303,125 - IAI46_20365 0.56 +1.0 4,303,134 + IAI46_20365 0.56 +0.2 4,303,135 - IAI46_20365 0.56 +0.1 4,303,137 - IAI46_20365 0.57 -0.1 4,303,160 + IAI46_20365 0.59 +1.1
Or see this region's nucleotide sequence