Experiment: Cobalt chloride 60 uM, anaerobic
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt IAI46_20185 and IAI46_20190 are separated by 86 nucleotides IAI46_20190 and IAI46_20195 overlap by 4 nucleotides IAI46_20195 and IAI46_20200 are separated by 3 nucleotides IAI46_20200 and IAI46_20205 are separated by 3 nucleotides
IAI46_20185: IAI46_20185 - DNA polymerase III subunit alpha, at 4,256,715 to 4,260,203
_20185
IAI46_20190: IAI46_20190 - ribonuclease HII, at 4,260,290 to 4,260,883
_20190
IAI46_20195: IAI46_20195 - lipid-A-disaccharide synthase, at 4,260,880 to 4,262,028
_20195
IAI46_20200: IAI46_20200 - acyl-ACP--UDP-N-acetylglucosamine O-acyltransferase, at 4,262,032 to 4,262,820
_20200
IAI46_20205: IAI46_20205 - 3-hydroxyacyl-ACP dehydratase FabZ, at 4,262,824 to 4,263,279
_20205
Position (kb) 4260
4261
4262
4263 Strain fitness (log2 ratio) -1
0
1 at 4260.292 kb on - strand at 4260.292 kb on - strand at 4260.395 kb on - strand, within IAI46_20190 at 4260.395 kb on - strand, within IAI46_20190 at 4260.554 kb on - strand, within IAI46_20190 at 4260.567 kb on - strand, within IAI46_20190 at 4260.584 kb on - strand, within IAI46_20190 at 4260.619 kb on + strand, within IAI46_20190 at 4260.709 kb on - strand, within IAI46_20190 at 4260.849 kb on - strand at 4260.849 kb on - strand at 4260.864 kb on + strand at 4260.865 kb on - strand at 4260.865 kb on - strand at 4260.865 kb on - strand at 4260.865 kb on - strand at 4260.865 kb on - strand at 4262.029 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Cobalt chloride 60 uM, anaerobic remove 4,260,292 - -0.4 4,260,292 - +0.1 4,260,395 - IAI46_20190 0.18 -0.4 4,260,395 - IAI46_20190 0.18 +0.3 4,260,554 - IAI46_20190 0.44 +0.2 4,260,567 - IAI46_20190 0.47 +0.2 4,260,584 - IAI46_20190 0.49 -0.4 4,260,619 + IAI46_20190 0.55 -0.6 4,260,709 - IAI46_20190 0.71 -0.6 4,260,849 - +0.6 4,260,849 - +0.1 4,260,864 + -0.1 4,260,865 - +0.2 4,260,865 - -1.1 4,260,865 - +0.1 4,260,865 - +1.3 4,260,865 - -1.1 4,262,029 - +0.6
Or see this region's nucleotide sequence