Experiment: Cobalt chloride 60 uM, anaerobic
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt IAI46_18375 and IAI46_18380 are separated by 139 nucleotides IAI46_18380 and IAI46_18385 are separated by 39 nucleotides
IAI46_18375: IAI46_18375 - ABC transporter substrate-binding protein, at 3,868,453 to 3,870,048
_18375
IAI46_18380: IAI46_18380 - acetolactate synthase AlsS, at 3,870,188 to 3,871,873
_18380
IAI46_18385: IAI46_18385 - acetolactate decarboxylase, at 3,871,913 to 3,872,692
_18385
Position (kb)
3870
3871
3872 Strain fitness (log2 ratio)
-1
0
1
2 at 3869.326 kb on - strand, within IAI46_18375 at 3869.369 kb on + strand, within IAI46_18375 at 3869.387 kb on + strand, within IAI46_18375 at 3869.387 kb on + strand, within IAI46_18375 at 3869.388 kb on - strand, within IAI46_18375 at 3869.412 kb on - strand, within IAI46_18375 at 3869.479 kb on + strand, within IAI46_18375 at 3869.479 kb on + strand, within IAI46_18375 at 3869.479 kb on + strand, within IAI46_18375 at 3869.495 kb on + strand, within IAI46_18375 at 3869.522 kb on + strand, within IAI46_18375 at 3869.522 kb on + strand, within IAI46_18375 at 3869.522 kb on + strand, within IAI46_18375 at 3869.523 kb on - strand, within IAI46_18375 at 3869.523 kb on - strand, within IAI46_18375 at 3869.645 kb on + strand, within IAI46_18375 at 3869.676 kb on + strand, within IAI46_18375 at 3869.742 kb on + strand, within IAI46_18375 at 3869.748 kb on - strand, within IAI46_18375 at 3869.748 kb on - strand, within IAI46_18375 at 3869.751 kb on + strand, within IAI46_18375 at 3869.752 kb on - strand, within IAI46_18375 at 3869.752 kb on - strand, within IAI46_18375 at 3869.882 kb on + strand, within IAI46_18375 at 3869.882 kb on + strand, within IAI46_18375 at 3869.923 kb on - strand at 3869.938 kb on - strand at 3870.073 kb on - strand at 3870.085 kb on + strand at 3870.091 kb on + strand at 3870.092 kb on - strand at 3870.104 kb on + strand at 3870.112 kb on + strand at 3870.113 kb on - strand at 3870.113 kb on - strand at 3870.189 kb on + strand at 3870.189 kb on + strand at 3870.229 kb on - strand at 3870.229 kb on - strand at 3870.358 kb on - strand, within IAI46_18380 at 3870.365 kb on - strand, within IAI46_18380 at 3870.405 kb on + strand, within IAI46_18380 at 3870.406 kb on - strand, within IAI46_18380 at 3870.406 kb on - strand, within IAI46_18380 at 3870.438 kb on + strand, within IAI46_18380 at 3870.473 kb on - strand, within IAI46_18380 at 3870.517 kb on + strand, within IAI46_18380 at 3870.721 kb on + strand, within IAI46_18380 at 3870.863 kb on - strand, within IAI46_18380 at 3870.934 kb on + strand, within IAI46_18380 at 3870.934 kb on + strand, within IAI46_18380 at 3870.962 kb on - strand, within IAI46_18380 at 3871.008 kb on + strand, within IAI46_18380 at 3871.120 kb on + strand, within IAI46_18380 at 3871.470 kb on + strand, within IAI46_18380 at 3871.471 kb on - strand, within IAI46_18380 at 3871.471 kb on - strand, within IAI46_18380 at 3871.471 kb on - strand, within IAI46_18380 at 3871.493 kb on - strand, within IAI46_18380 at 3871.684 kb on + strand, within IAI46_18380 at 3871.684 kb on + strand, within IAI46_18380 at 3871.787 kb on + strand at 3871.787 kb on + strand at 3871.787 kb on + strand at 3871.795 kb on + strand at 3871.849 kb on + strand at 3871.849 kb on + strand at 3871.849 kb on + strand at 3871.849 kb on + strand at 3871.850 kb on - strand at 3871.850 kb on - strand at 3871.879 kb on - strand at 3871.891 kb on + strand at 3872.050 kb on - strand, within IAI46_18385 at 3872.128 kb on + strand, within IAI46_18385 at 3872.128 kb on + strand, within IAI46_18385 at 3872.128 kb on + strand, within IAI46_18385 at 3872.128 kb on + strand, within IAI46_18385 at 3872.129 kb on - strand, within IAI46_18385 at 3872.130 kb on + strand, within IAI46_18385 at 3872.130 kb on + strand, within IAI46_18385 at 3872.131 kb on - strand, within IAI46_18385 at 3872.131 kb on - strand, within IAI46_18385 at 3872.131 kb on - strand, within IAI46_18385 at 3872.131 kb on - strand, within IAI46_18385 at 3872.131 kb on - strand, within IAI46_18385 at 3872.131 kb on - strand, within IAI46_18385 at 3872.131 kb on - strand, within IAI46_18385 at 3872.131 kb on - strand, within IAI46_18385 at 3872.198 kb on + strand, within IAI46_18385 at 3872.377 kb on + strand, within IAI46_18385 at 3872.378 kb on - strand, within IAI46_18385 at 3872.604 kb on + strand, within IAI46_18385 at 3872.696 kb on + strand at 3872.696 kb on + strand at 3872.696 kb on + strand at 3872.697 kb on - strand at 3872.697 kb on - strand at 3872.697 kb on - strand at 3872.751 kb on + strand at 3872.868 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Cobalt chloride 60 uM, anaerobic remove 3,869,326 - IAI46_18375 0.55 -0.4 3,869,369 + IAI46_18375 0.57 +0.3 3,869,387 + IAI46_18375 0.59 +0.4 3,869,387 + IAI46_18375 0.59 +0.2 3,869,388 - IAI46_18375 0.59 +0.5 3,869,412 - IAI46_18375 0.60 -0.1 3,869,479 + IAI46_18375 0.64 -0.0 3,869,479 + IAI46_18375 0.64 +0.7 3,869,479 + IAI46_18375 0.64 -0.9 3,869,495 + IAI46_18375 0.65 -0.6 3,869,522 + IAI46_18375 0.67 -0.1 3,869,522 + IAI46_18375 0.67 +0.4 3,869,522 + IAI46_18375 0.67 +1.0 3,869,523 - IAI46_18375 0.67 -1.1 3,869,523 - IAI46_18375 0.67 +1.0 3,869,645 + IAI46_18375 0.75 +0.0 3,869,676 + IAI46_18375 0.77 +2.1 3,869,742 + IAI46_18375 0.81 -0.2 3,869,748 - IAI46_18375 0.81 +0.5 3,869,748 - IAI46_18375 0.81 -1.3 3,869,751 + IAI46_18375 0.81 +0.2 3,869,752 - IAI46_18375 0.81 +0.9 3,869,752 - IAI46_18375 0.81 +1.4 3,869,882 + IAI46_18375 0.90 -0.3 3,869,882 + IAI46_18375 0.90 -0.6 3,869,923 - -1.2 3,869,938 - -0.1 3,870,073 - -0.3 3,870,085 + +0.1 3,870,091 + -0.4 3,870,092 - -0.5 3,870,104 + +1.0 3,870,112 + +0.3 3,870,113 - +0.2 3,870,113 - +1.3 3,870,189 + +0.0 3,870,189 + -1.0 3,870,229 - -0.3 3,870,229 - +1.0 3,870,358 - IAI46_18380 0.10 +0.7 3,870,365 - IAI46_18380 0.10 -0.1 3,870,405 + IAI46_18380 0.13 -0.3 3,870,406 - IAI46_18380 0.13 -0.5 3,870,406 - IAI46_18380 0.13 -0.9 3,870,438 + IAI46_18380 0.15 +2.1 3,870,473 - IAI46_18380 0.17 -0.0 3,870,517 + IAI46_18380 0.20 +0.1 3,870,721 + IAI46_18380 0.32 +0.7 3,870,863 - IAI46_18380 0.40 +0.3 3,870,934 + IAI46_18380 0.44 +0.6 3,870,934 + IAI46_18380 0.44 -0.7 3,870,962 - IAI46_18380 0.46 -0.1 3,871,008 + IAI46_18380 0.49 -0.2 3,871,120 + IAI46_18380 0.55 +1.3 3,871,470 + IAI46_18380 0.76 -0.3 3,871,471 - IAI46_18380 0.76 -0.5 3,871,471 - IAI46_18380 0.76 -0.5 3,871,471 - IAI46_18380 0.76 +0.1 3,871,493 - IAI46_18380 0.77 +0.1 3,871,684 + IAI46_18380 0.89 +1.8 3,871,684 + IAI46_18380 0.89 +0.4 3,871,787 + -0.2 3,871,787 + +0.4 3,871,787 + -0.1 3,871,795 + -0.2 3,871,849 + +0.1 3,871,849 + +0.3 3,871,849 + +0.0 3,871,849 + +0.1 3,871,850 - +0.6 3,871,850 - +0.8 3,871,879 - +0.5 3,871,891 + +0.5 3,872,050 - IAI46_18385 0.18 -0.1 3,872,128 + IAI46_18385 0.28 +0.6 3,872,128 + IAI46_18385 0.28 +0.1 3,872,128 + IAI46_18385 0.28 -0.7 3,872,128 + IAI46_18385 0.28 -0.9 3,872,129 - IAI46_18385 0.28 -0.3 3,872,130 + IAI46_18385 0.28 +0.9 3,872,130 + IAI46_18385 0.28 -0.2 3,872,131 - IAI46_18385 0.28 +0.2 3,872,131 - IAI46_18385 0.28 +0.4 3,872,131 - IAI46_18385 0.28 -1.4 3,872,131 - IAI46_18385 0.28 -0.3 3,872,131 - IAI46_18385 0.28 +0.2 3,872,131 - IAI46_18385 0.28 -0.5 3,872,131 - IAI46_18385 0.28 +0.3 3,872,131 - IAI46_18385 0.28 -0.5 3,872,198 + IAI46_18385 0.37 -0.1 3,872,377 + IAI46_18385 0.59 -0.4 3,872,378 - IAI46_18385 0.60 +2.2 3,872,604 + IAI46_18385 0.89 +0.1 3,872,696 + +0.0 3,872,696 + +0.1 3,872,696 + +0.1 3,872,697 - +1.0 3,872,697 - -0.5 3,872,697 - +0.1 3,872,751 + -0.2 3,872,868 + -0.4
Or see this region's nucleotide sequence