Experiment: Cobalt chloride 60 uM, anaerobic
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt IAI46_15560 and IAI46_15565 are separated by 139 nucleotides IAI46_15565 and IAI46_15570 overlap by 11 nucleotides IAI46_15570 and IAI46_15575 are separated by 4 nucleotides
IAI46_15560: IAI46_15560 - glycosyltransferase family 2 protein, at 3,260,614 to 3,261,519
_15560
IAI46_15565: IAI46_15565 - glycosyltransferase, at 3,261,659 to 3,262,507
_15565
IAI46_15570: IAI46_15570 - flippase, at 3,262,497 to 3,263,786
_15570
IAI46_15575: IAI46_15575 - polysaccharide biosynthesis tyrosine autokinase, at 3,263,791 to 3,265,971
_15575
Position (kb)
3262
3263
3264 Strain fitness (log2 ratio)
-1
0
1
2 at 3261.535 kb on + strand at 3261.660 kb on + strand at 3261.788 kb on + strand, within IAI46_15565 at 3261.792 kb on + strand, within IAI46_15565 at 3261.793 kb on - strand, within IAI46_15565 at 3262.069 kb on + strand, within IAI46_15565 at 3262.070 kb on - strand, within IAI46_15565 at 3262.077 kb on + strand, within IAI46_15565 at 3262.078 kb on - strand, within IAI46_15565 at 3262.148 kb on - strand, within IAI46_15565 at 3262.309 kb on + strand, within IAI46_15565 at 3262.326 kb on + strand, within IAI46_15565 at 3262.331 kb on - strand, within IAI46_15565 at 3262.345 kb on + strand, within IAI46_15565 at 3262.348 kb on - strand, within IAI46_15565 at 3262.363 kb on - strand, within IAI46_15565 at 3262.392 kb on + strand, within IAI46_15565 at 3262.393 kb on - strand, within IAI46_15565 at 3262.406 kb on - strand, within IAI46_15565 at 3262.445 kb on + strand at 3262.455 kb on + strand at 3262.456 kb on - strand at 3262.475 kb on - strand at 3262.507 kb on + strand at 3262.602 kb on + strand at 3262.675 kb on - strand, within IAI46_15570 at 3262.719 kb on + strand, within IAI46_15570 at 3262.834 kb on + strand, within IAI46_15570 at 3262.887 kb on + strand, within IAI46_15570 at 3262.888 kb on - strand, within IAI46_15570 at 3262.888 kb on - strand, within IAI46_15570 at 3262.890 kb on + strand, within IAI46_15570 at 3262.890 kb on + strand, within IAI46_15570 at 3262.924 kb on + strand, within IAI46_15570 at 3262.924 kb on + strand, within IAI46_15570 at 3262.925 kb on - strand, within IAI46_15570 at 3262.925 kb on - strand, within IAI46_15570 at 3262.925 kb on - strand, within IAI46_15570 at 3262.925 kb on - strand, within IAI46_15570 at 3262.925 kb on - strand, within IAI46_15570 at 3262.995 kb on - strand, within IAI46_15570 at 3263.078 kb on - strand, within IAI46_15570 at 3263.081 kb on + strand, within IAI46_15570 at 3263.108 kb on + strand, within IAI46_15570 at 3263.109 kb on - strand, within IAI46_15570 at 3263.110 kb on + strand, within IAI46_15570 at 3263.110 kb on + strand, within IAI46_15570 at 3263.111 kb on - strand, within IAI46_15570 at 3263.272 kb on + strand, within IAI46_15570 at 3263.273 kb on - strand, within IAI46_15570 at 3263.273 kb on - strand, within IAI46_15570 at 3263.396 kb on - strand, within IAI46_15570 at 3263.408 kb on - strand, within IAI46_15570 at 3263.428 kb on + strand, within IAI46_15570 at 3263.429 kb on - strand, within IAI46_15570 at 3263.787 kb on - strand at 3263.831 kb on + strand at 3263.832 kb on - strand at 3263.834 kb on + strand at 3263.835 kb on - strand at 3263.886 kb on + strand at 3264.204 kb on + strand, within IAI46_15575 at 3264.204 kb on + strand, within IAI46_15575 at 3264.204 kb on + strand, within IAI46_15575 at 3264.205 kb on - strand, within IAI46_15575 at 3264.205 kb on - strand, within IAI46_15575 at 3264.375 kb on + strand, within IAI46_15575 at 3264.376 kb on - strand, within IAI46_15575 at 3264.560 kb on + strand, within IAI46_15575 at 3264.561 kb on - strand, within IAI46_15575
Per-strain Table
Position Strand Gene LocusTag Fraction Cobalt chloride 60 uM, anaerobic remove 3,261,535 + -1.2 3,261,660 + +0.3 3,261,788 + IAI46_15565 0.15 +0.4 3,261,792 + IAI46_15565 0.16 -0.1 3,261,793 - IAI46_15565 0.16 +1.2 3,262,069 + IAI46_15565 0.48 +1.0 3,262,070 - IAI46_15565 0.48 -0.8 3,262,077 + IAI46_15565 0.49 +0.2 3,262,078 - IAI46_15565 0.49 -0.1 3,262,148 - IAI46_15565 0.58 -0.4 3,262,309 + IAI46_15565 0.77 +0.5 3,262,326 + IAI46_15565 0.79 +0.3 3,262,331 - IAI46_15565 0.79 +1.8 3,262,345 + IAI46_15565 0.81 -0.8 3,262,348 - IAI46_15565 0.81 +0.3 3,262,363 - IAI46_15565 0.83 -0.2 3,262,392 + IAI46_15565 0.86 -0.1 3,262,393 - IAI46_15565 0.86 +0.7 3,262,406 - IAI46_15565 0.88 +0.2 3,262,445 + -0.2 3,262,455 + -0.1 3,262,456 - +0.6 3,262,475 - -1.7 3,262,507 + +0.2 3,262,602 + -0.4 3,262,675 - IAI46_15570 0.14 +0.6 3,262,719 + IAI46_15570 0.17 -1.2 3,262,834 + IAI46_15570 0.26 +0.6 3,262,887 + IAI46_15570 0.30 -0.2 3,262,888 - IAI46_15570 0.30 -0.7 3,262,888 - IAI46_15570 0.30 +0.9 3,262,890 + IAI46_15570 0.30 -1.5 3,262,890 + IAI46_15570 0.30 +1.5 3,262,924 + IAI46_15570 0.33 -0.3 3,262,924 + IAI46_15570 0.33 +0.2 3,262,925 - IAI46_15570 0.33 -1.0 3,262,925 - IAI46_15570 0.33 -0.9 3,262,925 - IAI46_15570 0.33 +0.8 3,262,925 - IAI46_15570 0.33 -0.5 3,262,925 - IAI46_15570 0.33 +0.3 3,262,995 - IAI46_15570 0.39 -0.2 3,263,078 - IAI46_15570 0.45 +0.4 3,263,081 + IAI46_15570 0.45 +0.2 3,263,108 + IAI46_15570 0.47 +0.9 3,263,109 - IAI46_15570 0.47 +0.6 3,263,110 + IAI46_15570 0.48 -0.6 3,263,110 + IAI46_15570 0.48 +0.6 3,263,111 - IAI46_15570 0.48 +0.4 3,263,272 + IAI46_15570 0.60 -0.7 3,263,273 - IAI46_15570 0.60 -1.7 3,263,273 - IAI46_15570 0.60 +2.0 3,263,396 - IAI46_15570 0.70 +1.1 3,263,408 - IAI46_15570 0.71 -0.0 3,263,428 + IAI46_15570 0.72 +0.6 3,263,429 - IAI46_15570 0.72 +0.3 3,263,787 - +0.5 3,263,831 + -0.5 3,263,832 - +0.8 3,263,834 + -0.7 3,263,835 - +0.3 3,263,886 + +1.7 3,264,204 + IAI46_15575 0.19 +0.3 3,264,204 + IAI46_15575 0.19 -0.0 3,264,204 + IAI46_15575 0.19 -0.8 3,264,205 - IAI46_15575 0.19 +0.1 3,264,205 - IAI46_15575 0.19 -0.3 3,264,375 + IAI46_15575 0.27 +0.7 3,264,376 - IAI46_15575 0.27 +0.0 3,264,560 + IAI46_15575 0.35 -1.1 3,264,561 - IAI46_15575 0.35 +0.4
Or see this region's nucleotide sequence