Experiment: Cobalt chloride 60 uM, anaerobic
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt IAI46_14290 and IAI46_14295 are separated by 45 nucleotides IAI46_14295 and IAI46_14300 are separated by 18 nucleotides IAI46_14300 and IAI46_14305 overlap by 4 nucleotides IAI46_14305 and IAI46_14310 overlap by 1 nucleotides
IAI46_14290: IAI46_14290 - exodeoxyribonuclease III, at 3,032,372 to 3,033,178
_14290
IAI46_14295: IAI46_14295 - pyrimidine (deoxy)nucleoside triphosphate diphosphatase, at 3,033,224 to 3,033,625
_14295
IAI46_14300: IAI46_14300 - YnjH family protein, at 3,033,644 to 3,033,937
_14300
IAI46_14305: IAI46_14305 - DNA topoisomerase III, at 3,033,934 to 3,035,859
_14305
IAI46_14310: IAI46_14310 - tRNA 2-selenouridine(34) synthase MnmH, at 3,035,859 to 3,036,977
_14310
Position (kb)
3033
3034
3035
3036 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 3032.966 kb on + strand, within IAI46_14290 at 3033.113 kb on + strand at 3033.434 kb on - strand, within IAI46_14295 at 3033.434 kb on - strand, within IAI46_14295 at 3033.460 kb on + strand, within IAI46_14295 at 3033.465 kb on + strand, within IAI46_14295 at 3033.465 kb on + strand, within IAI46_14295 at 3033.596 kb on + strand at 3033.596 kb on + strand at 3033.597 kb on - strand at 3033.598 kb on + strand at 3033.609 kb on + strand at 3033.766 kb on - strand, within IAI46_14300 at 3034.218 kb on + strand, within IAI46_14305 at 3034.220 kb on + strand, within IAI46_14305 at 3034.221 kb on - strand, within IAI46_14305 at 3034.234 kb on - strand, within IAI46_14305 at 3034.235 kb on + strand, within IAI46_14305 at 3034.514 kb on - strand, within IAI46_14305 at 3034.652 kb on + strand, within IAI46_14305 at 3034.753 kb on - strand, within IAI46_14305 at 3035.025 kb on - strand, within IAI46_14305 at 3035.025 kb on - strand, within IAI46_14305 at 3035.114 kb on + strand, within IAI46_14305 at 3035.321 kb on + strand, within IAI46_14305 at 3035.321 kb on + strand, within IAI46_14305 at 3035.321 kb on + strand, within IAI46_14305 at 3035.406 kb on + strand, within IAI46_14305 at 3035.423 kb on - strand, within IAI46_14305 at 3035.700 kb on - strand at 3035.726 kb on - strand at 3035.861 kb on - strand at 3035.881 kb on + strand at 3035.881 kb on + strand at 3035.881 kb on + strand at 3035.881 kb on + strand at 3035.962 kb on + strand at 3035.962 kb on + strand at 3035.962 kb on + strand at 3035.977 kb on + strand, within IAI46_14310 at 3035.978 kb on - strand, within IAI46_14310 at 3036.035 kb on - strand, within IAI46_14310 at 3036.046 kb on - strand, within IAI46_14310 at 3036.130 kb on + strand, within IAI46_14310 at 3036.200 kb on + strand, within IAI46_14310 at 3036.201 kb on - strand, within IAI46_14310 at 3036.201 kb on - strand, within IAI46_14310 at 3036.202 kb on + strand, within IAI46_14310 at 3036.203 kb on - strand, within IAI46_14310 at 3036.373 kb on - strand, within IAI46_14310 at 3036.606 kb on - strand, within IAI46_14310 at 3036.689 kb on - strand, within IAI46_14310 at 3036.689 kb on - strand, within IAI46_14310
Per-strain Table
Position Strand Gene LocusTag Fraction Cobalt chloride 60 uM, anaerobic remove 3,032,966 + IAI46_14290 0.74 -0.8 3,033,113 + -0.2 3,033,434 - IAI46_14295 0.52 -0.1 3,033,434 - IAI46_14295 0.52 -1.2 3,033,460 + IAI46_14295 0.59 +0.2 3,033,465 + IAI46_14295 0.60 +1.1 3,033,465 + IAI46_14295 0.60 +0.1 3,033,596 + -1.9 3,033,596 + +0.7 3,033,597 - -0.2 3,033,598 + +2.0 3,033,609 + -0.9 3,033,766 - IAI46_14300 0.41 +0.7 3,034,218 + IAI46_14305 0.15 -1.2 3,034,220 + IAI46_14305 0.15 -0.6 3,034,221 - IAI46_14305 0.15 -0.8 3,034,234 - IAI46_14305 0.16 -1.2 3,034,235 + IAI46_14305 0.16 +2.3 3,034,514 - IAI46_14305 0.30 +0.6 3,034,652 + IAI46_14305 0.37 -0.1 3,034,753 - IAI46_14305 0.43 +0.4 3,035,025 - IAI46_14305 0.57 +0.1 3,035,025 - IAI46_14305 0.57 +0.4 3,035,114 + IAI46_14305 0.61 -1.0 3,035,321 + IAI46_14305 0.72 -0.3 3,035,321 + IAI46_14305 0.72 +0.9 3,035,321 + IAI46_14305 0.72 -0.5 3,035,406 + IAI46_14305 0.76 +0.7 3,035,423 - IAI46_14305 0.77 +1.2 3,035,700 - +1.0 3,035,726 - -0.4 3,035,861 - +0.4 3,035,881 + +0.9 3,035,881 + -1.2 3,035,881 + -0.2 3,035,881 + +0.1 3,035,962 + -0.9 3,035,962 + +0.2 3,035,962 + +0.7 3,035,977 + IAI46_14310 0.11 -0.3 3,035,978 - IAI46_14310 0.11 -2.2 3,036,035 - IAI46_14310 0.16 -0.3 3,036,046 - IAI46_14310 0.17 -0.6 3,036,130 + IAI46_14310 0.24 +0.2 3,036,200 + IAI46_14310 0.30 +0.4 3,036,201 - IAI46_14310 0.31 +0.8 3,036,201 - IAI46_14310 0.31 +0.7 3,036,202 + IAI46_14310 0.31 -0.6 3,036,203 - IAI46_14310 0.31 -0.1 3,036,373 - IAI46_14310 0.46 -0.6 3,036,606 - IAI46_14310 0.67 +0.8 3,036,689 - IAI46_14310 0.74 +0.9 3,036,689 - IAI46_14310 0.74 +0.1
Or see this region's nucleotide sequence