Experiment: Cobalt chloride 60 uM, anaerobic
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt IAI46_10065 and IAI46_10070 overlap by 4 nucleotides IAI46_10070 and IAI46_10075 are separated by 160 nucleotides IAI46_10075 and IAI46_10080 are separated by 3 nucleotides IAI46_10080 and IAI46_10085 are separated by 122 nucleotides
IAI46_10065: IAI46_10065 - hypothetical protein, at 2,122,667 to 2,123,026
_10065
IAI46_10070: IAI46_10070 - LysR family transcriptional regulator, at 2,123,023 to 2,123,130
_10070
IAI46_10075: IAI46_10075 - two-component system response regulator DcuR, at 2,123,291 to 2,124,004
_10075
IAI46_10080: IAI46_10080 - two-component system sensor histidine kinase DcuS, at 2,124,008 to 2,125,609
_10080
IAI46_10085: IAI46_10085 - aldolase, at 2,125,732 to 2,126,574
_10085
Position (kb)
2124
2125
2126 Strain fitness (log2 ratio)
-1
0
1
2 at 2123.025 kb on - strand at 2123.092 kb on + strand, within IAI46_10070 at 2123.093 kb on - strand, within IAI46_10070 at 2123.222 kb on + strand at 2123.223 kb on - strand at 2123.228 kb on + strand at 2123.236 kb on + strand at 2123.237 kb on - strand at 2123.239 kb on + strand at 2123.240 kb on - strand at 2123.347 kb on - strand at 2123.409 kb on + strand, within IAI46_10075 at 2123.410 kb on - strand, within IAI46_10075 at 2123.410 kb on - strand, within IAI46_10075 at 2123.413 kb on + strand, within IAI46_10075 at 2123.414 kb on - strand, within IAI46_10075 at 2123.414 kb on - strand, within IAI46_10075 at 2123.414 kb on - strand, within IAI46_10075 at 2123.418 kb on + strand, within IAI46_10075 at 2123.419 kb on - strand, within IAI46_10075 at 2123.524 kb on + strand, within IAI46_10075 at 2123.647 kb on - strand, within IAI46_10075 at 2123.647 kb on - strand, within IAI46_10075 at 2123.713 kb on - strand, within IAI46_10075 at 2123.830 kb on + strand, within IAI46_10075 at 2123.831 kb on - strand, within IAI46_10075 at 2123.831 kb on - strand, within IAI46_10075 at 2123.832 kb on + strand, within IAI46_10075 at 2123.832 kb on + strand, within IAI46_10075 at 2123.832 kb on + strand, within IAI46_10075 at 2123.832 kb on - strand, within IAI46_10075 at 2123.833 kb on - strand, within IAI46_10075 at 2123.833 kb on - strand, within IAI46_10075 at 2123.833 kb on - strand, within IAI46_10075 at 2123.833 kb on - strand, within IAI46_10075 at 2123.833 kb on - strand, within IAI46_10075 at 2123.833 kb on - strand, within IAI46_10075 at 2123.833 kb on - strand, within IAI46_10075 at 2123.833 kb on - strand, within IAI46_10075 at 2123.947 kb on - strand at 2123.987 kb on - strand at 2123.999 kb on - strand at 2124.032 kb on - strand at 2124.053 kb on + strand at 2124.053 kb on + strand at 2124.224 kb on + strand, within IAI46_10080 at 2124.224 kb on + strand, within IAI46_10080 at 2124.225 kb on - strand, within IAI46_10080 at 2124.514 kb on - strand, within IAI46_10080 at 2124.531 kb on + strand, within IAI46_10080 at 2124.532 kb on - strand, within IAI46_10080 at 2124.541 kb on + strand, within IAI46_10080 at 2124.541 kb on + strand, within IAI46_10080 at 2124.542 kb on - strand, within IAI46_10080 at 2124.542 kb on - strand, within IAI46_10080 at 2124.612 kb on + strand, within IAI46_10080 at 2124.816 kb on + strand, within IAI46_10080 at 2124.817 kb on - strand, within IAI46_10080 at 2124.821 kb on - strand, within IAI46_10080 at 2124.901 kb on + strand, within IAI46_10080 at 2124.901 kb on + strand, within IAI46_10080 at 2124.902 kb on - strand, within IAI46_10080 at 2124.937 kb on + strand, within IAI46_10080 at 2124.937 kb on + strand, within IAI46_10080 at 2125.080 kb on + strand, within IAI46_10080 at 2125.080 kb on + strand, within IAI46_10080 at 2125.081 kb on - strand, within IAI46_10080 at 2125.084 kb on + strand, within IAI46_10080 at 2125.085 kb on - strand, within IAI46_10080 at 2125.151 kb on + strand, within IAI46_10080 at 2125.152 kb on - strand, within IAI46_10080 at 2125.546 kb on + strand at 2125.682 kb on - strand at 2125.683 kb on + strand at 2125.683 kb on + strand at 2125.684 kb on - strand at 2125.684 kb on - strand at 2125.684 kb on - strand at 2125.745 kb on + strand at 2125.746 kb on - strand at 2125.832 kb on + strand, within IAI46_10085 at 2125.833 kb on - strand, within IAI46_10085 at 2125.833 kb on - strand, within IAI46_10085 at 2126.075 kb on + strand, within IAI46_10085 at 2126.135 kb on + strand, within IAI46_10085 at 2126.278 kb on - strand, within IAI46_10085 at 2126.282 kb on - strand, within IAI46_10085 at 2126.318 kb on + strand, within IAI46_10085 at 2126.318 kb on + strand, within IAI46_10085 at 2126.399 kb on + strand, within IAI46_10085 at 2126.399 kb on + strand, within IAI46_10085 at 2126.439 kb on + strand, within IAI46_10085 at 2126.457 kb on + strand, within IAI46_10085 at 2126.559 kb on + strand at 2126.560 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Cobalt chloride 60 uM, anaerobic remove 2,123,025 - +0.5 2,123,092 + IAI46_10070 0.64 -1.7 2,123,093 - IAI46_10070 0.65 +1.4 2,123,222 + +0.7 2,123,223 - +0.1 2,123,228 + +0.0 2,123,236 + +0.3 2,123,237 - +0.6 2,123,239 + +0.1 2,123,240 - -0.2 2,123,347 - +0.5 2,123,409 + IAI46_10075 0.17 +1.2 2,123,410 - IAI46_10075 0.17 +0.7 2,123,410 - IAI46_10075 0.17 +0.1 2,123,413 + IAI46_10075 0.17 +0.4 2,123,414 - IAI46_10075 0.17 +0.8 2,123,414 - IAI46_10075 0.17 +0.6 2,123,414 - IAI46_10075 0.17 -0.6 2,123,418 + IAI46_10075 0.18 -0.2 2,123,419 - IAI46_10075 0.18 +0.5 2,123,524 + IAI46_10075 0.33 -1.0 2,123,647 - IAI46_10075 0.50 -1.0 2,123,647 - IAI46_10075 0.50 -0.1 2,123,713 - IAI46_10075 0.59 +0.4 2,123,830 + IAI46_10075 0.75 +0.6 2,123,831 - IAI46_10075 0.76 -0.4 2,123,831 - IAI46_10075 0.76 -1.4 2,123,832 + IAI46_10075 0.76 +1.4 2,123,832 + IAI46_10075 0.76 -1.0 2,123,832 + IAI46_10075 0.76 -0.8 2,123,832 - IAI46_10075 0.76 +0.8 2,123,833 - IAI46_10075 0.76 +0.8 2,123,833 - IAI46_10075 0.76 +0.3 2,123,833 - IAI46_10075 0.76 -1.1 2,123,833 - IAI46_10075 0.76 +0.6 2,123,833 - IAI46_10075 0.76 -0.1 2,123,833 - IAI46_10075 0.76 +0.6 2,123,833 - IAI46_10075 0.76 -0.9 2,123,833 - IAI46_10075 0.76 -0.6 2,123,947 - +0.9 2,123,987 - +0.2 2,123,999 - +1.5 2,124,032 - +0.4 2,124,053 + +1.2 2,124,053 + +0.7 2,124,224 + IAI46_10080 0.13 -0.1 2,124,224 + IAI46_10080 0.13 -0.5 2,124,225 - IAI46_10080 0.14 +0.1 2,124,514 - IAI46_10080 0.32 -0.0 2,124,531 + IAI46_10080 0.33 +0.3 2,124,532 - IAI46_10080 0.33 +0.1 2,124,541 + IAI46_10080 0.33 -0.2 2,124,541 + IAI46_10080 0.33 +0.6 2,124,542 - IAI46_10080 0.33 +0.0 2,124,542 - IAI46_10080 0.33 -0.0 2,124,612 + IAI46_10080 0.38 +1.1 2,124,816 + IAI46_10080 0.50 -0.1 2,124,817 - IAI46_10080 0.50 +0.1 2,124,821 - IAI46_10080 0.51 -0.3 2,124,901 + IAI46_10080 0.56 +0.5 2,124,901 + IAI46_10080 0.56 +0.9 2,124,902 - IAI46_10080 0.56 +0.4 2,124,937 + IAI46_10080 0.58 -1.7 2,124,937 + IAI46_10080 0.58 +1.3 2,125,080 + IAI46_10080 0.67 -0.2 2,125,080 + IAI46_10080 0.67 +0.3 2,125,081 - IAI46_10080 0.67 -0.6 2,125,084 + IAI46_10080 0.67 -0.3 2,125,085 - IAI46_10080 0.67 +0.2 2,125,151 + IAI46_10080 0.71 +0.7 2,125,152 - IAI46_10080 0.71 -0.1 2,125,546 + +0.2 2,125,682 - -0.6 2,125,683 + +1.1 2,125,683 + +0.4 2,125,684 - +0.6 2,125,684 - +0.1 2,125,684 - +0.5 2,125,745 + +0.8 2,125,746 - +1.5 2,125,832 + IAI46_10085 0.12 -0.7 2,125,833 - IAI46_10085 0.12 +2.1 2,125,833 - IAI46_10085 0.12 -0.2 2,126,075 + IAI46_10085 0.41 +0.0 2,126,135 + IAI46_10085 0.48 -0.1 2,126,278 - IAI46_10085 0.65 +0.1 2,126,282 - IAI46_10085 0.65 -0.7 2,126,318 + IAI46_10085 0.70 -0.0 2,126,318 + IAI46_10085 0.70 +1.1 2,126,399 + IAI46_10085 0.79 +0.5 2,126,399 + IAI46_10085 0.79 -0.1 2,126,439 + IAI46_10085 0.84 -0.4 2,126,457 + IAI46_10085 0.86 +0.3 2,126,559 + +0.3 2,126,560 - +0.1
Or see this region's nucleotide sequence