Experiment: Cobalt chloride 60 uM, anaerobic
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt IAI46_05400 and IAI46_05405 are separated by 414 nucleotides IAI46_05405 and IAI46_05410 are separated by 145 nucleotides IAI46_05410 and IAI46_05415 are separated by 195 nucleotides
IAI46_05400: IAI46_05400 - bifunctional methylenetetrahydrofolate dehydrogenase/methenyltetrahydrofolate cyclohydrolase FolD, at 1,163,649 to 1,164,515
_05400
IAI46_05405: IAI46_05405 - hypothetical protein, at 1,164,930 to 1,165,226
_05405
IAI46_05410: IAI46_05410 - tRNA-Arg, at 1,165,372 to 1,165,448
_05410
IAI46_05415: IAI46_05415 - tyrosine-type recombinase/integrase, at 1,165,644 to 1,166,852
_05415
Position (kb)
1164
1165
1166 Strain fitness (log2 ratio)
-2
-1
0
1 at 1164.537 kb on - strand at 1164.559 kb on - strand at 1164.559 kb on - strand at 1164.685 kb on - strand at 1164.753 kb on - strand at 1164.753 kb on - strand at 1164.957 kb on - strand at 1164.967 kb on + strand, within IAI46_05405 at 1164.968 kb on - strand, within IAI46_05405 at 1164.995 kb on - strand, within IAI46_05405 at 1165.022 kb on - strand, within IAI46_05405 at 1165.151 kb on + strand, within IAI46_05405 at 1165.188 kb on - strand, within IAI46_05405 at 1165.188 kb on - strand, within IAI46_05405 at 1165.211 kb on - strand at 1165.274 kb on + strand at 1165.275 kb on - strand at 1165.503 kb on - strand at 1165.566 kb on - strand at 1165.570 kb on + strand at 1165.570 kb on + strand at 1165.570 kb on + strand at 1165.570 kb on + strand at 1165.571 kb on - strand at 1165.571 kb on - strand at 1165.571 kb on - strand at 1165.578 kb on + strand at 1165.578 kb on + strand at 1165.578 kb on + strand at 1165.579 kb on - strand at 1165.581 kb on - strand at 1165.581 kb on - strand at 1165.581 kb on - strand at 1165.581 kb on - strand at 1165.582 kb on + strand at 1165.582 kb on + strand at 1165.582 kb on + strand at 1165.582 kb on + strand at 1165.582 kb on + strand at 1165.582 kb on + strand at 1165.582 kb on + strand at 1165.582 kb on + strand at 1165.582 kb on + strand at 1165.582 kb on + strand at 1165.582 kb on + strand at 1165.582 kb on + strand at 1165.582 kb on + strand at 1165.583 kb on - strand at 1165.583 kb on - strand at 1165.583 kb on - strand at 1165.583 kb on - strand at 1165.583 kb on - strand at 1165.583 kb on - strand at 1165.583 kb on - strand at 1165.590 kb on + strand at 1165.590 kb on + strand at 1165.605 kb on + strand at 1165.606 kb on - strand at 1165.616 kb on - strand at 1165.660 kb on + strand at 1165.660 kb on + strand at 1165.661 kb on - strand at 1165.667 kb on - strand at 1165.695 kb on + strand at 1165.695 kb on + strand at 1165.695 kb on + strand at 1165.695 kb on + strand at 1165.696 kb on - strand at 1165.726 kb on + strand at 1165.752 kb on + strand at 1165.752 kb on + strand at 1165.762 kb on + strand at 1165.762 kb on + strand at 1165.763 kb on - strand at 1165.801 kb on + strand, within IAI46_05415 at 1165.805 kb on + strand, within IAI46_05415 at 1165.805 kb on + strand, within IAI46_05415 at 1165.805 kb on + strand, within IAI46_05415 at 1165.806 kb on - strand, within IAI46_05415 at 1165.806 kb on - strand, within IAI46_05415 at 1165.806 kb on - strand, within IAI46_05415 at 1165.877 kb on + strand, within IAI46_05415 at 1165.877 kb on + strand, within IAI46_05415 at 1165.878 kb on - strand, within IAI46_05415 at 1165.912 kb on + strand, within IAI46_05415 at 1165.912 kb on + strand, within IAI46_05415 at 1165.913 kb on - strand, within IAI46_05415 at 1165.931 kb on - strand, within IAI46_05415 at 1165.971 kb on + strand, within IAI46_05415 at 1166.013 kb on - strand, within IAI46_05415 at 1166.013 kb on - strand, within IAI46_05415 at 1166.034 kb on - strand, within IAI46_05415 at 1166.052 kb on - strand, within IAI46_05415 at 1166.072 kb on - strand, within IAI46_05415 at 1166.090 kb on + strand, within IAI46_05415 at 1166.091 kb on - strand, within IAI46_05415 at 1166.091 kb on - strand, within IAI46_05415 at 1166.157 kb on - strand, within IAI46_05415 at 1166.202 kb on - strand, within IAI46_05415
Per-strain Table
Position Strand Gene LocusTag Fraction Cobalt chloride 60 uM, anaerobic remove 1,164,537 - +0.5 1,164,559 - +0.7 1,164,559 - -0.4 1,164,685 - +1.1 1,164,753 - +0.3 1,164,753 - +0.7 1,164,957 - -0.4 1,164,967 + IAI46_05405 0.12 +1.1 1,164,968 - IAI46_05405 0.13 +0.6 1,164,995 - IAI46_05405 0.22 -0.9 1,165,022 - IAI46_05405 0.31 +0.7 1,165,151 + IAI46_05405 0.74 -0.5 1,165,188 - IAI46_05405 0.87 +0.0 1,165,188 - IAI46_05405 0.87 -0.1 1,165,211 - +0.0 1,165,274 + -1.2 1,165,275 - +0.6 1,165,503 - -0.6 1,165,566 - -1.2 1,165,570 + -1.4 1,165,570 + -1.1 1,165,570 + +0.1 1,165,570 + +0.5 1,165,571 - -0.6 1,165,571 - +0.1 1,165,571 - -1.2 1,165,578 + -0.2 1,165,578 + -0.7 1,165,578 + -0.1 1,165,579 - -1.1 1,165,581 - -0.3 1,165,581 - -0.5 1,165,581 - -1.0 1,165,581 - -0.7 1,165,582 + -0.4 1,165,582 + -0.1 1,165,582 + +0.3 1,165,582 + -0.2 1,165,582 + +0.3 1,165,582 + -0.1 1,165,582 + -0.5 1,165,582 + +0.7 1,165,582 + +0.2 1,165,582 + +0.0 1,165,582 + +0.5 1,165,582 + +1.4 1,165,582 + +0.1 1,165,583 - -0.2 1,165,583 - +0.1 1,165,583 - +0.4 1,165,583 - +0.4 1,165,583 - -1.1 1,165,583 - +0.3 1,165,583 - -0.3 1,165,590 + +0.6 1,165,590 + +0.1 1,165,605 + +0.1 1,165,606 - +0.5 1,165,616 - -2.4 1,165,660 + +0.4 1,165,660 + +0.7 1,165,661 - -0.3 1,165,667 - -0.9 1,165,695 + +0.4 1,165,695 + +0.3 1,165,695 + +0.9 1,165,695 + -0.2 1,165,696 - +0.1 1,165,726 + +0.0 1,165,752 + +0.4 1,165,752 + -0.6 1,165,762 + +0.4 1,165,762 + +0.8 1,165,763 - +0.3 1,165,801 + IAI46_05415 0.13 +0.5 1,165,805 + IAI46_05415 0.13 +0.9 1,165,805 + IAI46_05415 0.13 -0.3 1,165,805 + IAI46_05415 0.13 +1.1 1,165,806 - IAI46_05415 0.13 -0.0 1,165,806 - IAI46_05415 0.13 +0.5 1,165,806 - IAI46_05415 0.13 +0.9 1,165,877 + IAI46_05415 0.19 -0.6 1,165,877 + IAI46_05415 0.19 +0.3 1,165,878 - IAI46_05415 0.19 +0.7 1,165,912 + IAI46_05415 0.22 -0.6 1,165,912 + IAI46_05415 0.22 -0.1 1,165,913 - IAI46_05415 0.22 -0.7 1,165,931 - IAI46_05415 0.24 -1.9 1,165,971 + IAI46_05415 0.27 +0.0 1,166,013 - IAI46_05415 0.31 -0.7 1,166,013 - IAI46_05415 0.31 -0.4 1,166,034 - IAI46_05415 0.32 -0.9 1,166,052 - IAI46_05415 0.34 -0.6 1,166,072 - IAI46_05415 0.35 -0.3 1,166,090 + IAI46_05415 0.37 +0.2 1,166,091 - IAI46_05415 0.37 +0.2 1,166,091 - IAI46_05415 0.37 -0.2 1,166,157 - IAI46_05415 0.42 +0.7 1,166,202 - IAI46_05415 0.46 +0.2
Or see this region's nucleotide sequence