Experiment: Cobalt chloride 60 uM, anaerobic
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt IAI46_03475 and IAI46_03480 are separated by 26 nucleotides IAI46_03480 and IAI46_03485 are separated by 90 nucleotides IAI46_03485 and IAI46_03490 are separated by 101 nucleotides IAI46_03490 and IAI46_03495 are separated by 41 nucleotides IAI46_03495 and IAI46_03500 are separated by 50 nucleotides
IAI46_03475: IAI46_03475 - DUF721 domain-containing protein, at 753,671 to 754,195
_03475
IAI46_03480: IAI46_03480 - secA regulator SecM, at 754,222 to 754,755
_03480
IAI46_03485: IAI46_03485 - preprotein translocase subunit SecA, at 754,846 to 757,557
_03485
IAI46_03490: IAI46_03490 - 8-oxo-dGTP diphosphatase MutT, at 757,659 to 758,063
_03490
IAI46_03495: IAI46_03495 - DNA gyrase inhibitor YacG, at 758,105 to 758,296
_03495
IAI46_03500: IAI46_03500 - cell division protein ZapD, at 758,347 to 759,099
_03500
Position (kb) 754
755
756
757
758 Strain fitness (log2 ratio) -1
0
1
2 at 754.235 kb on + strand at 754.235 kb on + strand at 754.264 kb on + strand at 754.264 kb on + strand at 754.748 kb on + strand at 757.423 kb on + strand at 757.423 kb on + strand at 757.639 kb on - strand at 757.647 kb on - strand at 757.742 kb on + strand, within IAI46_03490 at 757.743 kb on - strand, within IAI46_03490 at 757.883 kb on + strand, within IAI46_03490 at 757.910 kb on - strand, within IAI46_03490 at 757.912 kb on - strand, within IAI46_03490 at 757.961 kb on + strand, within IAI46_03490 at 757.961 kb on + strand, within IAI46_03490 at 757.962 kb on - strand, within IAI46_03490 at 757.973 kb on - strand, within IAI46_03490 at 758.015 kb on + strand, within IAI46_03490 at 758.016 kb on - strand, within IAI46_03490 at 758.016 kb on - strand, within IAI46_03490 at 758.025 kb on - strand at 758.061 kb on + strand at 758.062 kb on - strand at 758.062 kb on - strand at 758.064 kb on + strand at 758.065 kb on - strand at 758.065 kb on - strand at 758.296 kb on + strand at 758.296 kb on + strand at 758.296 kb on + strand at 758.296 kb on + strand at 758.296 kb on + strand at 758.296 kb on + strand at 758.297 kb on - strand at 758.297 kb on - strand at 758.300 kb on + strand at 758.300 kb on + strand at 758.300 kb on + strand at 758.300 kb on + strand at 758.301 kb on - strand at 758.308 kb on - strand at 758.372 kb on - strand at 758.372 kb on - strand at 758.372 kb on - strand at 758.427 kb on - strand, within IAI46_03500 at 758.436 kb on + strand, within IAI46_03500 at 758.437 kb on - strand, within IAI46_03500 at 758.446 kb on + strand, within IAI46_03500 at 758.447 kb on - strand, within IAI46_03500 at 758.456 kb on + strand, within IAI46_03500 at 758.457 kb on - strand, within IAI46_03500 at 758.482 kb on + strand, within IAI46_03500 at 758.483 kb on - strand, within IAI46_03500 at 758.483 kb on - strand, within IAI46_03500
Per-strain Table
Position Strand Gene LocusTag Fraction Cobalt chloride 60 uM, anaerobic remove 754,235 + +1.1 754,235 + -0.3 754,264 + +0.4 754,264 + +0.1 754,748 + +1.5 757,423 + -0.5 757,423 + +0.7 757,639 - -0.5 757,647 - -0.7 757,742 + IAI46_03490 0.20 +0.3 757,743 - IAI46_03490 0.21 -0.5 757,883 + IAI46_03490 0.55 -0.4 757,910 - IAI46_03490 0.62 -0.3 757,912 - IAI46_03490 0.62 -0.5 757,961 + IAI46_03490 0.75 -1.0 757,961 + IAI46_03490 0.75 -0.9 757,962 - IAI46_03490 0.75 +0.8 757,973 - IAI46_03490 0.78 +0.4 758,015 + IAI46_03490 0.88 -0.7 758,016 - IAI46_03490 0.88 +0.8 758,016 - IAI46_03490 0.88 +0.6 758,025 - +0.4 758,061 + +1.9 758,062 - +0.4 758,062 - -0.1 758,064 + +0.1 758,065 - +0.3 758,065 - -0.3 758,296 + -1.0 758,296 + -0.4 758,296 + +0.2 758,296 + +0.8 758,296 + -0.4 758,296 + +0.2 758,297 - -1.3 758,297 - +0.5 758,300 + -0.5 758,300 + +0.0 758,300 + +0.9 758,300 + +0.4 758,301 - -0.4 758,308 - +0.3 758,372 - +0.9 758,372 - +0.8 758,372 - +1.2 758,427 - IAI46_03500 0.11 +0.6 758,436 + IAI46_03500 0.12 -0.6 758,437 - IAI46_03500 0.12 -0.2 758,446 + IAI46_03500 0.13 +0.8 758,447 - IAI46_03500 0.13 -0.2 758,456 + IAI46_03500 0.14 +0.6 758,457 - IAI46_03500 0.15 +0.0 758,482 + IAI46_03500 0.18 -0.8 758,483 - IAI46_03500 0.18 -0.3 758,483 - IAI46_03500 0.18 +1.2
Or see this region's nucleotide sequence