Experiment: Cobalt chloride 60 uM, anaerobic
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt IAI46_00215 and IAI46_00220 overlap by 4 nucleotides IAI46_00220 and IAI46_00225 overlap by 1 nucleotides IAI46_00225 and IAI46_00230 are separated by 228 nucleotides IAI46_00230 and IAI46_00235 are separated by 33 nucleotides
IAI46_00215: IAI46_00215 - formate dehydrogenase subunit beta, at 50,164 to 51,063
_00215
IAI46_00220: IAI46_00220 - formate dehydrogenase cytochrome b556 subunit, at 51,060 to 51,695
_00220
IAI46_00225: IAI46_00225 - formate dehydrogenase accessory protein FdhE, at 51,695 to 52,624
_00225
IAI46_00230: IAI46_00230 - DcrB-related protein, at 52,853 to 53,500
_00230
IAI46_00235: IAI46_00235 - glutathione S-transferase, at 53,534 to 54,139
_00235
Position (kb)
51
52
53 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 50.786 kb on - strand, within IAI46_00215 at 50.804 kb on - strand, within IAI46_00215 at 50.835 kb on + strand, within IAI46_00215 at 50.835 kb on + strand, within IAI46_00215 at 50.835 kb on + strand, within IAI46_00215 at 50.835 kb on + strand, within IAI46_00215 at 50.835 kb on + strand, within IAI46_00215 at 50.835 kb on + strand, within IAI46_00215 at 50.836 kb on - strand, within IAI46_00215 at 50.836 kb on - strand, within IAI46_00215 at 50.836 kb on - strand, within IAI46_00215 at 50.839 kb on + strand, within IAI46_00215 at 50.839 kb on + strand, within IAI46_00215 at 50.839 kb on + strand, within IAI46_00215 at 50.839 kb on + strand, within IAI46_00215 at 50.839 kb on + strand, within IAI46_00215 at 50.839 kb on + strand, within IAI46_00215 at 50.839 kb on + strand, within IAI46_00215 at 50.840 kb on - strand, within IAI46_00215 at 50.840 kb on - strand, within IAI46_00215 at 50.840 kb on - strand, within IAI46_00215 at 50.840 kb on - strand, within IAI46_00215 at 50.840 kb on - strand, within IAI46_00215 at 50.872 kb on + strand, within IAI46_00215 at 50.872 kb on + strand, within IAI46_00215 at 50.873 kb on - strand, within IAI46_00215 at 50.982 kb on - strand at 50.982 kb on - strand at 50.989 kb on + strand at 50.989 kb on + strand at 51.077 kb on + strand at 51.248 kb on + strand, within IAI46_00220 at 51.249 kb on - strand, within IAI46_00220 at 51.249 kb on - strand, within IAI46_00220 at 51.293 kb on + strand, within IAI46_00220 at 51.293 kb on + strand, within IAI46_00220 at 51.293 kb on + strand, within IAI46_00220 at 51.293 kb on + strand, within IAI46_00220 at 51.294 kb on - strand, within IAI46_00220 at 51.338 kb on + strand, within IAI46_00220 at 51.339 kb on - strand, within IAI46_00220 at 51.339 kb on - strand, within IAI46_00220 at 51.380 kb on + strand, within IAI46_00220 at 51.465 kb on + strand, within IAI46_00220 at 51.465 kb on + strand, within IAI46_00220 at 51.551 kb on + strand, within IAI46_00220 at 51.551 kb on + strand, within IAI46_00220 at 51.551 kb on + strand, within IAI46_00220 at 51.551 kb on + strand, within IAI46_00220 at 51.551 kb on + strand, within IAI46_00220 at 51.564 kb on + strand, within IAI46_00220 at 51.565 kb on - strand, within IAI46_00220 at 51.565 kb on - strand, within IAI46_00220 at 51.565 kb on - strand, within IAI46_00220 at 51.581 kb on - strand, within IAI46_00220 at 51.581 kb on - strand, within IAI46_00220 at 51.716 kb on - strand at 51.716 kb on - strand at 51.726 kb on + strand at 51.827 kb on - strand, within IAI46_00225 at 51.827 kb on - strand, within IAI46_00225 at 51.857 kb on + strand, within IAI46_00225 at 51.858 kb on - strand, within IAI46_00225 at 51.932 kb on - strand, within IAI46_00225 at 51.932 kb on - strand, within IAI46_00225 at 52.199 kb on + strand, within IAI46_00225 at 52.199 kb on + strand, within IAI46_00225 at 52.200 kb on - strand, within IAI46_00225 at 52.247 kb on + strand, within IAI46_00225 at 52.310 kb on - strand, within IAI46_00225 at 52.335 kb on - strand, within IAI46_00225 at 52.388 kb on - strand, within IAI46_00225 at 52.388 kb on - strand, within IAI46_00225 at 52.388 kb on - strand, within IAI46_00225 at 52.388 kb on - strand, within IAI46_00225 at 52.388 kb on - strand, within IAI46_00225 at 52.418 kb on + strand, within IAI46_00225 at 52.419 kb on - strand, within IAI46_00225 at 52.481 kb on + strand, within IAI46_00225 at 52.481 kb on + strand, within IAI46_00225 at 52.481 kb on + strand, within IAI46_00225 at 52.482 kb on - strand, within IAI46_00225 at 52.651 kb on + strand at 52.652 kb on - strand at 52.682 kb on - strand at 52.682 kb on - strand at 52.682 kb on - strand at 52.682 kb on - strand at 52.682 kb on - strand at 52.691 kb on - strand at 52.691 kb on - strand at 52.747 kb on + strand at 52.748 kb on - strand at 52.755 kb on + strand at 52.755 kb on + strand at 52.760 kb on - strand at 52.760 kb on - strand at 52.807 kb on + strand at 52.807 kb on + strand at 52.808 kb on - strand at 52.808 kb on - strand at 52.808 kb on - strand at 52.808 kb on - strand at 52.813 kb on + strand at 52.930 kb on + strand, within IAI46_00230 at 53.221 kb on + strand, within IAI46_00230 at 53.222 kb on - strand, within IAI46_00230 at 53.273 kb on + strand, within IAI46_00230 at 53.281 kb on + strand, within IAI46_00230 at 53.370 kb on + strand, within IAI46_00230 at 53.370 kb on + strand, within IAI46_00230 at 53.370 kb on + strand, within IAI46_00230 at 53.371 kb on - strand, within IAI46_00230 at 53.371 kb on - strand, within IAI46_00230 at 53.371 kb on - strand, within IAI46_00230 at 53.371 kb on - strand, within IAI46_00230 at 53.371 kb on - strand, within IAI46_00230 at 53.371 kb on - strand, within IAI46_00230 at 53.377 kb on + strand, within IAI46_00230 at 53.378 kb on - strand, within IAI46_00230 at 53.378 kb on - strand, within IAI46_00230 at 53.378 kb on - strand, within IAI46_00230 at 53.378 kb on - strand, within IAI46_00230 at 53.378 kb on - strand, within IAI46_00230 at 53.378 kb on - strand, within IAI46_00230 at 53.498 kb on + strand at 53.498 kb on + strand at 53.542 kb on + strand at 53.543 kb on - strand at 53.543 kb on - strand at 53.553 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Cobalt chloride 60 uM, anaerobic remove 50,786 - IAI46_00215 0.69 +0.3 50,804 - IAI46_00215 0.71 +0.1 50,835 + IAI46_00215 0.75 +1.5 50,835 + IAI46_00215 0.75 -0.4 50,835 + IAI46_00215 0.75 -1.1 50,835 + IAI46_00215 0.75 -0.0 50,835 + IAI46_00215 0.75 -0.6 50,835 + IAI46_00215 0.75 -0.1 50,836 - IAI46_00215 0.75 +1.0 50,836 - IAI46_00215 0.75 +2.7 50,836 - IAI46_00215 0.75 +0.9 50,839 + IAI46_00215 0.75 +1.2 50,839 + IAI46_00215 0.75 +1.1 50,839 + IAI46_00215 0.75 -0.5 50,839 + IAI46_00215 0.75 -0.2 50,839 + IAI46_00215 0.75 -0.7 50,839 + IAI46_00215 0.75 +0.4 50,839 + IAI46_00215 0.75 -0.1 50,840 - IAI46_00215 0.75 +0.1 50,840 - IAI46_00215 0.75 -0.5 50,840 - IAI46_00215 0.75 -1.1 50,840 - IAI46_00215 0.75 -0.1 50,840 - IAI46_00215 0.75 +0.9 50,872 + IAI46_00215 0.79 +0.1 50,872 + IAI46_00215 0.79 -0.8 50,873 - IAI46_00215 0.79 +0.7 50,982 - -2.0 50,982 - -0.3 50,989 + -0.2 50,989 + -0.3 51,077 + +0.4 51,248 + IAI46_00220 0.30 -0.0 51,249 - IAI46_00220 0.30 -0.4 51,249 - IAI46_00220 0.30 +0.0 51,293 + IAI46_00220 0.37 -0.1 51,293 + IAI46_00220 0.37 +0.2 51,293 + IAI46_00220 0.37 -0.2 51,293 + IAI46_00220 0.37 -0.2 51,294 - IAI46_00220 0.37 +0.8 51,338 + IAI46_00220 0.44 +0.6 51,339 - IAI46_00220 0.44 -0.1 51,339 - IAI46_00220 0.44 -0.5 51,380 + IAI46_00220 0.50 +0.8 51,465 + IAI46_00220 0.64 +0.2 51,465 + IAI46_00220 0.64 +0.6 51,551 + IAI46_00220 0.77 -0.3 51,551 + IAI46_00220 0.77 -0.4 51,551 + IAI46_00220 0.77 +0.4 51,551 + IAI46_00220 0.77 +0.6 51,551 + IAI46_00220 0.77 +0.5 51,564 + IAI46_00220 0.79 +0.8 51,565 - IAI46_00220 0.79 -0.1 51,565 - IAI46_00220 0.79 +0.8 51,565 - IAI46_00220 0.79 +0.8 51,581 - IAI46_00220 0.82 -0.2 51,581 - IAI46_00220 0.82 -0.5 51,716 - +0.9 51,716 - +0.7 51,726 + -0.1 51,827 - IAI46_00225 0.14 -0.3 51,827 - IAI46_00225 0.14 -0.0 51,857 + IAI46_00225 0.17 -0.3 51,858 - IAI46_00225 0.18 -0.5 51,932 - IAI46_00225 0.25 +0.8 51,932 - IAI46_00225 0.25 +0.5 52,199 + IAI46_00225 0.54 +0.3 52,199 + IAI46_00225 0.54 -0.4 52,200 - IAI46_00225 0.54 +1.4 52,247 + IAI46_00225 0.59 -0.2 52,310 - IAI46_00225 0.66 +0.1 52,335 - IAI46_00225 0.69 +0.0 52,388 - IAI46_00225 0.75 +0.2 52,388 - IAI46_00225 0.75 -0.4 52,388 - IAI46_00225 0.75 -1.2 52,388 - IAI46_00225 0.75 -1.2 52,388 - IAI46_00225 0.75 +0.5 52,418 + IAI46_00225 0.78 -0.4 52,419 - IAI46_00225 0.78 -0.6 52,481 + IAI46_00225 0.85 +0.4 52,481 + IAI46_00225 0.85 -0.3 52,481 + IAI46_00225 0.85 -0.2 52,482 - IAI46_00225 0.85 +0.4 52,651 + +0.2 52,652 - -0.1 52,682 - -0.7 52,682 - +1.4 52,682 - -0.6 52,682 - +0.4 52,682 - +0.4 52,691 - +0.1 52,691 - -0.2 52,747 + -0.1 52,748 - +0.4 52,755 + -0.2 52,755 + +0.2 52,760 - -0.2 52,760 - +0.1 52,807 + -0.1 52,807 + +0.5 52,808 - -0.8 52,808 - -1.4 52,808 - +0.4 52,808 - -0.6 52,813 + -0.4 52,930 + IAI46_00230 0.12 +0.5 53,221 + IAI46_00230 0.57 +0.2 53,222 - IAI46_00230 0.57 +0.8 53,273 + IAI46_00230 0.65 +0.5 53,281 + IAI46_00230 0.66 +0.5 53,370 + IAI46_00230 0.80 -0.0 53,370 + IAI46_00230 0.80 -0.1 53,370 + IAI46_00230 0.80 +0.7 53,371 - IAI46_00230 0.80 +0.0 53,371 - IAI46_00230 0.80 +0.2 53,371 - IAI46_00230 0.80 +0.9 53,371 - IAI46_00230 0.80 -0.3 53,371 - IAI46_00230 0.80 -0.1 53,371 - IAI46_00230 0.80 -0.3 53,377 + IAI46_00230 0.81 +0.2 53,378 - IAI46_00230 0.81 -0.5 53,378 - IAI46_00230 0.81 +0.8 53,378 - IAI46_00230 0.81 -2.0 53,378 - IAI46_00230 0.81 -0.5 53,378 - IAI46_00230 0.81 -1.1 53,378 - IAI46_00230 0.81 +0.9 53,498 + +0.1 53,498 + +0.5 53,542 + +0.5 53,543 - +0.0 53,543 - -1.6 53,553 - -0.6
Or see this region's nucleotide sequence