Experiment: L-Leucine (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ABID97_RS13195 and ABID97_RS13200 are separated by 19 nucleotides ABID97_RS13200 and ABID97_RS13205 are separated by 12 nucleotides ABID97_RS13205 and ABID97_RS13210 overlap by 4 nucleotides
ABID97_RS13195: ABID97_RS13195 - maleylacetate reductase, at 2,748,615 to 2,749,691
_RS13195
ABID97_RS13200: ABID97_RS13200 - intradiol ring-cleavage dioxygenase, at 2,749,711 to 2,750,559
_RS13200
ABID97_RS13205: ABID97_RS13205 - tripartite tricarboxylate transporter substrate binding protein, at 2,750,572 to 2,751,534
_RS13205
ABID97_RS13210: ABID97_RS13210 - FAD-dependent oxidoreductase, at 2,751,531 to 2,753,258
_RS13210
Position (kb)
2750
2751
2752 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2749.769 kb on + strand at 2749.769 kb on + strand at 2751.173 kb on + strand, within ABID97_RS13205 at 2751.174 kb on - strand, within ABID97_RS13205 at 2751.379 kb on + strand, within ABID97_RS13205 at 2751.380 kb on - strand, within ABID97_RS13205 at 2751.380 kb on - strand, within ABID97_RS13205 at 2751.677 kb on - strand at 2751.883 kb on + strand, within ABID97_RS13210 at 2751.883 kb on + strand, within ABID97_RS13210 at 2752.468 kb on + strand, within ABID97_RS13210 at 2752.528 kb on + strand, within ABID97_RS13210 at 2752.529 kb on - strand, within ABID97_RS13210
Per-strain Table
Position Strand Gene LocusTag Fraction L-Leucine (C) remove 2,749,769 + -1.9 2,749,769 + -0.4 2,751,173 + ABID97_RS13205 0.62 +1.5 2,751,174 - ABID97_RS13205 0.63 -0.1 2,751,379 + ABID97_RS13205 0.84 +0.4 2,751,380 - ABID97_RS13205 0.84 +1.8 2,751,380 - ABID97_RS13205 0.84 +1.0 2,751,677 - +0.8 2,751,883 + ABID97_RS13210 0.20 +0.5 2,751,883 + ABID97_RS13210 0.20 +0.2 2,752,468 + ABID97_RS13210 0.54 -0.8 2,752,528 + ABID97_RS13210 0.58 +0.3 2,752,529 - ABID97_RS13210 0.58 +2.1
Or see this region's nucleotide sequence