Strain Fitness in Escherichia coli BW25113 around b2780

Experiment: D-Alanine (N)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 nteno and pyrG are separated by 87 nucleotidespyrG and mazG are separated by 227 nucleotides b2779: eno - phosphopyruvate hydratase (NCBI), at 2,904,665 to 2,905,963 eno b2780: pyrG - CTP synthetase (NCBI), at 2,906,051 to 2,907,688 pyrG b2781: mazG - nucleoside triphosphate pyrophosphohydrolase (NCBI), at 2,907,916 to 2,908,707 mazG Position (kb) 2906 2907 2908Strain fitness (log2 ratio) -1 0 1at 2907.991 kb on - strandat 2908.016 kb on - strand, within mazGat 2908.022 kb on - strand, within mazGat 2908.082 kb on + strand, within mazGat 2908.122 kb on - strand, within mazGat 2908.157 kb on - strand, within mazGat 2908.172 kb on + strand, within mazGat 2908.172 kb on + strand, within mazGat 2908.377 kb on - strand, within mazGat 2908.538 kb on + strand, within mazGat 2908.571 kb on + strand, within mazGat 2908.624 kb on + strand, within mazGat 2908.674 kb on + strandat 2908.674 kb on + strandat 2908.674 kb on + strandat 2908.674 kb on + strandat 2908.682 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction D-Alanine (N)
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2,907,991 - +0.1
2,908,016 - mazG b2781 0.13 -0.3
2,908,022 - mazG b2781 0.13 +0.2
2,908,082 + mazG b2781 0.21 -0.2
2,908,122 - mazG b2781 0.26 +0.5
2,908,157 - mazG b2781 0.30 -0.4
2,908,172 + mazG b2781 0.32 +0.2
2,908,172 + mazG b2781 0.32 +0.1
2,908,377 - mazG b2781 0.58 -0.0
2,908,538 + mazG b2781 0.79 +0.2
2,908,571 + mazG b2781 0.83 +0.4
2,908,624 + mazG b2781 0.89 -0.2
2,908,674 + +0.1
2,908,674 + +0.1
2,908,674 + +0.3
2,908,674 + -0.1
2,908,682 - +0.2

Or see this region's nucleotide sequence