Experiment: D-Alanine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt hisD and hisC overlap by 4 nucleotides hisC and hisB overlap by 1 nucleotides
b2020: hisD - histidinol dehydrogenase (NCBI), at 2,089,121 to 2,090,425
hisD
b2021: hisC - histidinol-phosphate aminotransferase (NCBI), at 2,090,422 to 2,091,492
hisC
b2022: hisB - imidazoleglycerolphosphate dehydratase and histidinol-phosphate phosphatase (VIMSS), at 2,091,492 to 2,092,559
hisB
Position (kb)
2090
2091
2092 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1 at 2089.504 kb on + strand, within hisD at 2089.605 kb on - strand, within hisD at 2089.755 kb on - strand, within hisD at 2089.776 kb on + strand, within hisD at 2089.844 kb on + strand, within hisD at 2089.929 kb on - strand, within hisD at 2089.929 kb on - strand, within hisD at 2090.018 kb on - strand, within hisD at 2090.018 kb on - strand, within hisD at 2090.075 kb on - strand, within hisD at 2090.124 kb on + strand, within hisD at 2090.124 kb on + strand, within hisD at 2090.132 kb on - strand, within hisD at 2090.300 kb on - strand at 2090.337 kb on - strand at 2090.419 kb on + strand at 2090.721 kb on - strand, within hisC at 2090.808 kb on - strand, within hisC at 2090.932 kb on + strand, within hisC at 2090.932 kb on + strand, within hisC at 2090.938 kb on + strand, within hisC at 2091.102 kb on + strand, within hisC at 2091.202 kb on - strand, within hisC at 2091.202 kb on - strand, within hisC at 2091.274 kb on + strand, within hisC at 2091.351 kb on + strand, within hisC at 2091.593 kb on - strand at 2091.593 kb on - strand at 2091.619 kb on - strand, within hisB at 2091.659 kb on + strand, within hisB at 2091.985 kb on - strand, within hisB at 2092.013 kb on - strand, within hisB at 2092.013 kb on - strand, within hisB at 2092.070 kb on + strand, within hisB at 2092.080 kb on + strand, within hisB at 2092.114 kb on - strand, within hisB at 2092.160 kb on + strand, within hisB at 2092.171 kb on - strand, within hisB at 2092.193 kb on - strand, within hisB at 2092.193 kb on - strand, within hisB at 2092.355 kb on + strand, within hisB at 2092.355 kb on + strand, within hisB at 2092.359 kb on - strand, within hisB at 2092.406 kb on + strand, within hisB at 2092.406 kb on + strand, within hisB at 2092.412 kb on + strand, within hisB at 2092.412 kb on + strand, within hisB at 2092.464 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction D-Alanine (N) remove 2,089,504 + hisD b2020 0.29 -1.9 2,089,605 - hisD b2020 0.37 -3.9 2,089,755 - hisD b2020 0.49 -2.6 2,089,776 + hisD b2020 0.50 -5.5 2,089,844 + hisD b2020 0.55 -3.4 2,089,929 - hisD b2020 0.62 -3.4 2,089,929 - hisD b2020 0.62 -3.2 2,090,018 - hisD b2020 0.69 -1.1 2,090,018 - hisD b2020 0.69 -2.1 2,090,075 - hisD b2020 0.73 -2.6 2,090,124 + hisD b2020 0.77 -4.4 2,090,124 + hisD b2020 0.77 -3.5 2,090,132 - hisD b2020 0.77 -3.6 2,090,300 - -2.2 2,090,337 - -3.2 2,090,419 + -1.3 2,090,721 - hisC b2021 0.28 -1.3 2,090,808 - hisC b2021 0.36 -2.9 2,090,932 + hisC b2021 0.48 -4.2 2,090,932 + hisC b2021 0.48 -2.9 2,090,938 + hisC b2021 0.48 -2.4 2,091,102 + hisC b2021 0.63 -3.3 2,091,202 - hisC b2021 0.73 -3.1 2,091,202 - hisC b2021 0.73 -1.5 2,091,274 + hisC b2021 0.80 -2.6 2,091,351 + hisC b2021 0.87 -3.8 2,091,593 - +1.5 2,091,593 - -1.9 2,091,619 - hisB b2022 0.12 -4.0 2,091,659 + hisB b2022 0.16 -3.4 2,091,985 - hisB b2022 0.46 -3.0 2,092,013 - hisB b2022 0.49 -1.4 2,092,013 - hisB b2022 0.49 -4.5 2,092,070 + hisB b2022 0.54 -3.8 2,092,080 + hisB b2022 0.55 -2.5 2,092,114 - hisB b2022 0.58 -3.7 2,092,160 + hisB b2022 0.63 -2.4 2,092,171 - hisB b2022 0.64 -1.4 2,092,193 - hisB b2022 0.66 -2.9 2,092,193 - hisB b2022 0.66 -2.8 2,092,355 + hisB b2022 0.81 -3.4 2,092,355 + hisB b2022 0.81 -2.7 2,092,359 - hisB b2022 0.81 -3.7 2,092,406 + hisB b2022 0.86 -1.1 2,092,406 + hisB b2022 0.86 -2.9 2,092,412 + hisB b2022 0.86 -2.8 2,092,412 + hisB b2022 0.86 -2.6 2,092,464 + -3.3
Or see this region's nucleotide sequence