Experiment: D-Alanine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt setA and leuD are separated by 48 nucleotides leuD and leuC are separated by 10 nucleotides leuC and leuB are separated by 2 nucleotides
b0070: setA - broad specificity sugar efflux system (NCBI), at 77,621 to 78,799
setA
b0071: leuD - isopropylmalate isomerase small subunit (NCBI), at 78,848 to 79,453
leuD
b0072: leuC - isopropylmalate isomerase large subunit (NCBI), at 79,464 to 80,864
leuC
b0073: leuB - 3-isopropylmalate dehydrogenase (VIMSS), at 80,867 to 81,958
leuB
Position (kb)
79
80
81 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1
2 at 78.536 kb on - strand, within setA at 78.548 kb on - strand, within setA at 78.556 kb on + strand, within setA at 78.581 kb on + strand, within setA at 78.599 kb on + strand, within setA at 78.613 kb on + strand, within setA at 78.613 kb on + strand, within setA at 78.646 kb on + strand, within setA at 78.646 kb on + strand, within setA at 78.680 kb on - strand, within setA at 78.687 kb on + strand at 78.726 kb on + strand at 78.729 kb on - strand at 78.731 kb on + strand at 78.819 kb on - strand at 78.819 kb on - strand at 78.913 kb on + strand, within leuD at 78.913 kb on + strand, within leuD at 78.913 kb on + strand, within leuD at 78.913 kb on + strand, within leuD at 78.913 kb on + strand, within leuD at 78.931 kb on - strand, within leuD at 78.957 kb on + strand, within leuD at 79.062 kb on - strand, within leuD at 79.113 kb on + strand, within leuD at 79.149 kb on + strand, within leuD at 79.187 kb on - strand, within leuD at 79.187 kb on - strand, within leuD at 79.245 kb on + strand, within leuD at 79.253 kb on - strand, within leuD at 79.317 kb on + strand, within leuD at 79.317 kb on + strand, within leuD at 79.322 kb on + strand, within leuD at 79.462 kb on - strand at 79.465 kb on + strand at 79.530 kb on + strand at 79.596 kb on + strand at 79.596 kb on + strand at 79.607 kb on + strand, within leuC at 79.724 kb on + strand, within leuC at 79.739 kb on - strand, within leuC at 79.739 kb on - strand, within leuC at 79.787 kb on + strand, within leuC at 79.821 kb on + strand, within leuC at 79.821 kb on + strand, within leuC at 79.865 kb on + strand, within leuC at 79.865 kb on + strand, within leuC at 79.883 kb on + strand, within leuC at 79.888 kb on + strand, within leuC at 79.896 kb on - strand, within leuC at 79.931 kb on + strand, within leuC at 80.049 kb on - strand, within leuC at 80.049 kb on - strand, within leuC at 80.077 kb on + strand, within leuC at 80.077 kb on + strand, within leuC at 80.092 kb on - strand, within leuC at 80.120 kb on - strand, within leuC at 80.120 kb on - strand, within leuC at 80.141 kb on + strand, within leuC at 80.141 kb on + strand, within leuC at 80.165 kb on + strand, within leuC at 80.210 kb on + strand, within leuC at 80.210 kb on + strand, within leuC at 80.218 kb on - strand, within leuC at 80.218 kb on - strand, within leuC at 80.236 kb on - strand, within leuC at 80.349 kb on + strand, within leuC at 80.387 kb on + strand at 80.387 kb on + strand, within leuC at 80.414 kb on - strand, within leuC at 80.438 kb on - strand, within leuC at 80.671 kb on - strand, within leuC at 80.671 kb on - strand, within leuC at 80.671 kb on - strand, within leuC at 80.671 kb on - strand, within leuC at 80.714 kb on - strand, within leuC at 80.714 kb on - strand, within leuC at 80.785 kb on + strand at 80.785 kb on + strand at 80.893 kb on - strand at 80.893 kb on - strand at 80.918 kb on + strand at 80.918 kb on + strand at 80.929 kb on - strand at 80.965 kb on + strand at 80.973 kb on - strand at 80.986 kb on - strand, within leuB at 81.032 kb on - strand, within leuB at 81.037 kb on + strand, within leuB at 81.114 kb on + strand, within leuB at 81.114 kb on + strand, within leuB at 81.122 kb on - strand, within leuB at 81.191 kb on - strand, within leuB at 81.191 kb on - strand, within leuB at 81.224 kb on + strand, within leuB at 81.284 kb on + strand, within leuB at 81.284 kb on + strand, within leuB at 81.287 kb on + strand, within leuB at 81.290 kb on - strand, within leuB at 81.292 kb on - strand, within leuB at 81.292 kb on - strand, within leuB at 81.314 kb on - strand, within leuB at 81.404 kb on + strand, within leuB at 81.409 kb on - strand, within leuB at 81.457 kb on + strand, within leuB at 81.480 kb on + strand, within leuB at 81.554 kb on - strand, within leuB at 81.580 kb on + strand, within leuB at 81.607 kb on - strand, within leuB at 81.692 kb on + strand, within leuB at 81.692 kb on + strand, within leuB
Per-strain Table
Position Strand Gene LocusTag Fraction D-Alanine (N) remove 78,536 - setA b0070 0.78 +0.3 78,548 - setA b0070 0.79 +0.2 78,556 + setA b0070 0.79 -0.1 78,581 + setA b0070 0.81 +1.8 78,599 + setA b0070 0.83 +0.1 78,613 + setA b0070 0.84 +0.2 78,613 + setA b0070 0.84 +0.2 78,646 + setA b0070 0.87 -0.0 78,646 + setA b0070 0.87 +0.2 78,680 - setA b0070 0.90 -0.2 78,687 + +0.0 78,726 + -0.6 78,729 - -0.0 78,731 + +0.1 78,819 - -1.3 78,819 - +1.5 78,913 + leuD b0071 0.11 -1.4 78,913 + leuD b0071 0.11 -4.1 78,913 + leuD b0071 0.11 -2.1 78,913 + leuD b0071 0.11 -1.2 78,913 + leuD b0071 0.11 -2.2 78,931 - leuD b0071 0.14 -3.2 78,957 + leuD b0071 0.18 -4.4 79,062 - leuD b0071 0.35 -3.0 79,113 + leuD b0071 0.44 -3.5 79,149 + leuD b0071 0.50 -3.6 79,187 - leuD b0071 0.56 -1.8 79,187 - leuD b0071 0.56 -3.0 79,245 + leuD b0071 0.66 -2.6 79,253 - leuD b0071 0.67 -3.1 79,317 + leuD b0071 0.77 -3.4 79,317 + leuD b0071 0.77 -1.3 79,322 + leuD b0071 0.78 -3.6 79,462 - -0.5 79,465 + -3.8 79,530 + -1.4 79,596 + -3.0 79,596 + -2.1 79,607 + leuC b0072 0.10 -2.8 79,724 + leuC b0072 0.19 -3.3 79,739 - leuC b0072 0.20 -3.8 79,739 - leuC b0072 0.20 -2.6 79,787 + leuC b0072 0.23 -2.2 79,821 + leuC b0072 0.25 -3.7 79,821 + leuC b0072 0.25 -2.3 79,865 + leuC b0072 0.29 -1.8 79,865 + leuC b0072 0.29 -2.3 79,883 + leuC b0072 0.30 -1.9 79,888 + leuC b0072 0.30 -4.6 79,896 - leuC b0072 0.31 -4.5 79,931 + leuC b0072 0.33 -1.2 80,049 - leuC b0072 0.42 -2.8 80,049 - leuC b0072 0.42 -3.6 80,077 + leuC b0072 0.44 -5.2 80,077 + leuC b0072 0.44 -3.6 80,092 - leuC b0072 0.45 -3.6 80,120 - leuC b0072 0.47 -2.4 80,120 - leuC b0072 0.47 -1.6 80,141 + leuC b0072 0.48 -2.7 80,141 + leuC b0072 0.48 -3.5 80,165 + leuC b0072 0.50 -3.4 80,210 + leuC b0072 0.53 -2.0 80,210 + leuC b0072 0.53 -1.2 80,218 - leuC b0072 0.54 -4.0 80,218 - leuC b0072 0.54 -3.5 80,236 - leuC b0072 0.55 -3.8 80,349 + leuC b0072 0.63 -2.6 80,387 + +0.2 80,387 + leuC b0072 0.66 -1.6 80,414 - leuC b0072 0.68 -4.5 80,438 - leuC b0072 0.70 -2.0 80,671 - leuC b0072 0.86 -3.7 80,671 - leuC b0072 0.86 -2.8 80,671 - leuC b0072 0.86 -3.7 80,671 - leuC b0072 0.86 -2.9 80,714 - leuC b0072 0.89 -1.8 80,714 - leuC b0072 0.89 -1.7 80,785 + -2.2 80,785 + -2.5 80,893 - -1.2 80,893 - -2.1 80,918 + -3.6 80,918 + -3.5 80,929 - -2.1 80,965 + -2.6 80,973 - -3.6 80,986 - leuB b0073 0.11 -4.7 81,032 - leuB b0073 0.15 -2.8 81,037 + leuB b0073 0.16 -3.4 81,114 + leuB b0073 0.23 -0.8 81,114 + leuB b0073 0.23 -2.6 81,122 - leuB b0073 0.23 -3.4 81,191 - leuB b0073 0.30 -2.2 81,191 - leuB b0073 0.30 -2.1 81,224 + leuB b0073 0.33 -4.3 81,284 + leuB b0073 0.38 -2.5 81,284 + leuB b0073 0.38 -2.3 81,287 + leuB b0073 0.38 -2.1 81,290 - leuB b0073 0.39 -3.5 81,292 - leuB b0073 0.39 -3.7 81,292 - leuB b0073 0.39 -4.2 81,314 - leuB b0073 0.41 -3.1 81,404 + leuB b0073 0.49 -3.4 81,409 - leuB b0073 0.50 -1.8 81,457 + leuB b0073 0.54 -1.4 81,480 + leuB b0073 0.56 -2.4 81,554 - leuB b0073 0.63 -3.9 81,580 + leuB b0073 0.65 -4.2 81,607 - leuB b0073 0.68 -2.3 81,692 + leuB b0073 0.76 -2.8 81,692 + leuB b0073 0.76 -4.1
Or see this region's nucleotide sequence