Strain Fitness in Escherichia coli BW25113 around b2286

Experiment: D-Serine (N)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntnuoF and nuoE overlap by 4 nucleotidesnuoE and nuoC are separated by 2 nucleotidesnuoC and nuoB are separated by 105 nucleotidesnuoB and nuoA are separated by 15 nucleotides b2284: nuoF - NADH:ubiquinone oxidoreductase, chain F (NCBI), at 2,398,240 to 2,399,577 nuoF b2285: nuoE - NADH dehydrogenase subunit E (NCBI), at 2,399,574 to 2,400,074 nuoE b2286: nuoC - NADH:ubiquinone oxidoreductase, chain C,D (RefSeq), at 2,400,077 to 2,401,867 nuoC b2287: nuoB - NADH dehydrogenase subunit B (NCBI), at 2,401,973 to 2,402,635 nuoB b2288: nuoA - NADH dehydrogenase I chain A (VIMSS), at 2,402,651 to 2,403,094 nuoA Position (kb) 2400 2401 2402Strain fitness (log2 ratio) -1 0 1 2 3at 2399.113 kb on - strand, within nuoFat 2399.283 kb on - strand, within nuoFat 2399.293 kb on - strand, within nuoFat 2399.314 kb on - strand, within nuoFat 2399.405 kb on - strand, within nuoFat 2399.405 kb on - strand, within nuoFat 2399.406 kb on - strand, within nuoFat 2399.461 kb on + strandat 2399.461 kb on + strandat 2399.508 kb on - strandat 2399.626 kb on + strandat 2399.626 kb on + strand, within nuoEat 2399.725 kb on + strand, within nuoEat 2399.863 kb on - strand, within nuoEat 2399.948 kb on - strand, within nuoEat 2399.972 kb on + strand, within nuoEat 2400.031 kb on - strandat 2400.113 kb on + strandat 2400.113 kb on + strandat 2400.221 kb on - strandat 2400.221 kb on - strandat 2400.254 kb on + strandat 2400.254 kb on - strandat 2400.327 kb on - strand, within nuoCat 2400.349 kb on - strand, within nuoCat 2400.357 kb on - strand, within nuoCat 2400.431 kb on + strand, within nuoCat 2400.565 kb on - strand, within nuoCat 2400.683 kb on - strand, within nuoCat 2400.683 kb on - strand, within nuoCat 2401.060 kb on + strand, within nuoCat 2401.073 kb on - strand, within nuoCat 2401.073 kb on - strand, within nuoCat 2401.078 kb on - strand, within nuoCat 2401.197 kb on + strand, within nuoCat 2401.420 kb on - strand, within nuoCat 2401.492 kb on + strand, within nuoCat 2401.492 kb on + strand, within nuoCat 2401.504 kb on - strand, within nuoCat 2401.625 kb on + strand, within nuoCat 2401.665 kb on + strand, within nuoCat 2401.730 kb on - strandat 2401.730 kb on - strandat 2401.732 kb on - strandat 2401.732 kb on - strandat 2401.762 kb on - strandat 2401.767 kb on - strandat 2402.000 kb on + strandat 2402.085 kb on - strand, within nuoBat 2402.169 kb on + strand, within nuoBat 2402.169 kb on + strand, within nuoBat 2402.179 kb on - strand, within nuoBat 2402.212 kb on - strand, within nuoBat 2402.284 kb on + strand, within nuoBat 2402.378 kb on - strand, within nuoBat 2402.386 kb on - strand, within nuoBat 2402.386 kb on - strand, within nuoBat 2402.434 kb on + strand, within nuoBat 2402.434 kb on + strand, within nuoBat 2402.583 kb on + strandat 2402.621 kb on - strandat 2402.624 kb on + strandat 2402.662 kb on + strandat 2402.662 kb on + strandat 2402.665 kb on + strandat 2402.665 kb on + strandat 2402.694 kb on - strandat 2402.723 kb on + strand, within nuoAat 2402.723 kb on + strand, within nuoAat 2402.745 kb on + strandat 2402.805 kb on - strand, within nuoAat 2402.805 kb on - strand, within nuoAat 2402.819 kb on + strand, within nuoA

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Per-strain Table

Position Strand Gene LocusTag Fraction D-Serine (N)
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2,399,113 - nuoF b2284 0.65 +1.3
2,399,283 - nuoF b2284 0.78 -0.3
2,399,293 - nuoF b2284 0.79 +2.0
2,399,314 - nuoF b2284 0.80 +1.6
2,399,405 - nuoF b2284 0.87 +1.6
2,399,405 - nuoF b2284 0.87 +0.8
2,399,406 - nuoF b2284 0.87 -0.4
2,399,461 + +0.4
2,399,461 + -0.0
2,399,508 - +1.0
2,399,626 + +1.1
2,399,626 + nuoE b2285 0.10 -0.3
2,399,725 + nuoE b2285 0.30 +1.3
2,399,863 - nuoE b2285 0.58 -0.4
2,399,948 - nuoE b2285 0.75 +1.6
2,399,972 + nuoE b2285 0.79 +0.5
2,400,031 - +0.2
2,400,113 + -0.3
2,400,113 + +0.7
2,400,221 - +0.1
2,400,221 - +1.7
2,400,254 + +1.1
2,400,254 - +0.6
2,400,327 - nuoC b2286 0.14 +0.4
2,400,349 - nuoC b2286 0.15 +0.9
2,400,357 - nuoC b2286 0.16 +1.6
2,400,431 + nuoC b2286 0.20 +0.2
2,400,565 - nuoC b2286 0.27 -1.2
2,400,683 - nuoC b2286 0.34 +0.0
2,400,683 - nuoC b2286 0.34 +1.1
2,401,060 + nuoC b2286 0.55 +1.4
2,401,073 - nuoC b2286 0.56 +0.3
2,401,073 - nuoC b2286 0.56 +1.1
2,401,078 - nuoC b2286 0.56 +0.7
2,401,197 + nuoC b2286 0.63 +1.1
2,401,420 - nuoC b2286 0.75 +0.6
2,401,492 + nuoC b2286 0.79 +0.6
2,401,492 + nuoC b2286 0.79 +1.4
2,401,504 - nuoC b2286 0.80 -0.8
2,401,625 + nuoC b2286 0.86 +0.4
2,401,665 + nuoC b2286 0.89 +1.5
2,401,730 - +1.2
2,401,730 - +0.8
2,401,732 - +0.2
2,401,732 - -0.1
2,401,762 - +0.3
2,401,767 - +2.1
2,402,000 + +1.2
2,402,085 - nuoB b2287 0.17 +0.7
2,402,169 + nuoB b2287 0.30 +3.7
2,402,169 + nuoB b2287 0.30 +1.2
2,402,179 - nuoB b2287 0.31 +2.0
2,402,212 - nuoB b2287 0.36 -0.1
2,402,284 + nuoB b2287 0.47 +0.2
2,402,378 - nuoB b2287 0.61 +0.4
2,402,386 - nuoB b2287 0.62 +0.8
2,402,386 - nuoB b2287 0.62 -0.0
2,402,434 + nuoB b2287 0.70 +0.4
2,402,434 + nuoB b2287 0.70 +0.5
2,402,583 + +0.6
2,402,621 - +1.6
2,402,624 + +1.4
2,402,662 + +0.1
2,402,662 + +0.6
2,402,665 + +1.6
2,402,665 + +1.3
2,402,694 - +0.5
2,402,723 + nuoA b2288 0.16 +2.1
2,402,723 + nuoA b2288 0.16 +0.6
2,402,745 + +3.1
2,402,805 - nuoA b2288 0.35 +1.0
2,402,805 - nuoA b2288 0.35 +1.5
2,402,819 + nuoA b2288 0.38 +0.9

Or see this region's nucleotide sequence