Strain Fitness in Escherichia coli BW25113 around b1602

Experiment: D-Serine (N)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntydgG and pntB are separated by 24 nucleotidespntB and pntA are separated by 10 nucleotides b1601: ydgG - predicted inner membrane protein (NCBI), at 1,671,937 to 1,672,971 ydgG b1602: pntB - pyridine nucleotide transhydrogenase (NCBI), at 1,672,996 to 1,674,384 pntB b1603: pntA - NAD(P) transhydrogenase subunit alpha (NCBI), at 1,674,395 to 1,675,927 pntA Position (kb) 1672 1673 1674 1675Strain fitness (log2 ratio) -3 -2 -1 0 1at 1672.027 kb on + strandat 1672.082 kb on - strand, within ydgGat 1672.082 kb on - strand, within ydgGat 1672.095 kb on - strand, within ydgGat 1672.095 kb on - strand, within ydgGat 1672.095 kb on - strand, within ydgGat 1672.102 kb on - strand, within ydgGat 1672.106 kb on + strand, within ydgGat 1672.151 kb on - strand, within ydgGat 1672.157 kb on - strand, within ydgGat 1672.291 kb on - strand, within ydgGat 1672.365 kb on + strand, within ydgGat 1672.365 kb on + strand, within ydgGat 1672.413 kb on + strand, within ydgGat 1672.481 kb on + strand, within ydgGat 1672.668 kb on - strand, within ydgGat 1672.698 kb on - strand, within ydgGat 1672.698 kb on - strand, within ydgGat 1672.796 kb on + strand, within ydgGat 1673.042 kb on + strandat 1673.095 kb on - strandat 1673.102 kb on - strandat 1673.145 kb on + strand, within pntBat 1673.501 kb on - strand, within pntBat 1673.579 kb on - strand, within pntBat 1673.579 kb on - strand, within pntBat 1673.667 kb on + strand, within pntBat 1673.672 kb on + strand, within pntBat 1673.672 kb on + strand, within pntBat 1673.878 kb on + strand, within pntBat 1673.878 kb on + strand, within pntBat 1674.216 kb on + strand, within pntBat 1674.370 kb on - strandat 1674.385 kb on + strandat 1674.511 kb on - strandat 1674.521 kb on - strandat 1674.521 kb on - strandat 1674.523 kb on + strandat 1674.583 kb on - strand, within pntAat 1674.865 kb on + strand, within pntAat 1674.894 kb on - strand, within pntAat 1675.064 kb on + strand, within pntAat 1675.064 kb on + strand, within pntA

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Per-strain Table

Position Strand Gene LocusTag Fraction D-Serine (N)
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1,672,027 + -0.1
1,672,082 - ydgG b1601 0.14 +0.4
1,672,082 - ydgG b1601 0.14 -0.3
1,672,095 - ydgG b1601 0.15 +1.0
1,672,095 - ydgG b1601 0.15 +0.2
1,672,095 - ydgG b1601 0.15 -0.2
1,672,102 - ydgG b1601 0.16 +0.7
1,672,106 + ydgG b1601 0.16 +0.4
1,672,151 - ydgG b1601 0.21 +0.4
1,672,157 - ydgG b1601 0.21 +0.1
1,672,291 - ydgG b1601 0.34 -0.5
1,672,365 + ydgG b1601 0.41 +0.5
1,672,365 + ydgG b1601 0.41 -0.6
1,672,413 + ydgG b1601 0.46 -0.6
1,672,481 + ydgG b1601 0.53 -0.3
1,672,668 - ydgG b1601 0.71 -0.1
1,672,698 - ydgG b1601 0.74 +0.7
1,672,698 - ydgG b1601 0.74 -0.8
1,672,796 + ydgG b1601 0.83 -0.1
1,673,042 + -1.9
1,673,095 - -2.2
1,673,102 - -1.8
1,673,145 + pntB b1602 0.11 -0.6
1,673,501 - pntB b1602 0.36 -0.7
1,673,579 - pntB b1602 0.42 -0.1
1,673,579 - pntB b1602 0.42 -2.0
1,673,667 + pntB b1602 0.48 -1.5
1,673,672 + pntB b1602 0.49 +0.1
1,673,672 + pntB b1602 0.49 -1.3
1,673,878 + pntB b1602 0.63 -1.9
1,673,878 + pntB b1602 0.63 -1.7
1,674,216 + pntB b1602 0.88 -2.6
1,674,370 - -2.9
1,674,385 + -1.6
1,674,511 - -1.2
1,674,521 - -1.0
1,674,521 - +0.3
1,674,523 + -0.5
1,674,583 - pntA b1603 0.12 -2.2
1,674,865 + pntA b1603 0.31 -1.0
1,674,894 - pntA b1603 0.33 -0.8
1,675,064 + pntA b1603 0.44 -2.0
1,675,064 + pntA b1603 0.44 +0.3

Or see this region's nucleotide sequence