Experiment: L-Proline (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ABIE41_RS22460 and ABIE41_RS22465 are separated by 220 nucleotides ABIE41_RS22465 and ABIE41_RS22470 are separated by 337 nucleotides ABIE41_RS22470 and ABIE41_RS22475 are separated by 13 nucleotides
ABIE41_RS22460: ABIE41_RS22460 - helix-turn-helix domain-containing protein, at 4,622,664 to 4,623,002
_RS22460
ABIE41_RS22465: ABIE41_RS22465 - MucR family transcriptional regulator, at 4,623,223 to 4,623,639
_RS22465
ABIE41_RS22470: ABIE41_RS22470 - S9 family peptidase, at 4,623,977 to 4,626,130
_RS22470
ABIE41_RS22475: ABIE41_RS22475 - hypothetical protein, at 4,626,144 to 4,627,586
_RS22475
Position (kb)
4623
4624
4625
4626
4627 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 4623.290 kb on + strand, within ABIE41_RS22465 at 4623.290 kb on + strand, within ABIE41_RS22465 at 4623.290 kb on + strand, within ABIE41_RS22465 at 4623.341 kb on + strand, within ABIE41_RS22465 at 4623.362 kb on + strand, within ABIE41_RS22465 at 4623.363 kb on - strand, within ABIE41_RS22465 at 4623.363 kb on - strand, within ABIE41_RS22465 at 4623.363 kb on - strand, within ABIE41_RS22465 at 4623.581 kb on + strand, within ABIE41_RS22465 at 4623.581 kb on + strand, within ABIE41_RS22465 at 4623.582 kb on - strand, within ABIE41_RS22465 at 4623.615 kb on - strand at 4623.659 kb on - strand at 4623.662 kb on + strand at 4623.662 kb on + strand at 4623.663 kb on - strand at 4623.741 kb on - strand at 4623.944 kb on - strand at 4623.944 kb on - strand at 4624.094 kb on + strand at 4624.095 kb on - strand at 4624.095 kb on - strand at 4624.169 kb on + strand at 4624.170 kb on - strand at 4624.170 kb on - strand at 4624.191 kb on - strand at 4624.307 kb on + strand, within ABIE41_RS22470 at 4624.307 kb on + strand, within ABIE41_RS22470 at 4624.308 kb on - strand, within ABIE41_RS22470 at 4624.310 kb on + strand, within ABIE41_RS22470 at 4624.311 kb on - strand, within ABIE41_RS22470 at 4624.311 kb on - strand, within ABIE41_RS22470 at 4624.320 kb on - strand, within ABIE41_RS22470 at 4624.430 kb on + strand, within ABIE41_RS22470 at 4624.610 kb on + strand, within ABIE41_RS22470 at 4624.610 kb on + strand, within ABIE41_RS22470 at 4624.610 kb on + strand, within ABIE41_RS22470 at 4624.610 kb on + strand, within ABIE41_RS22470 at 4624.611 kb on - strand, within ABIE41_RS22470 at 4624.650 kb on + strand, within ABIE41_RS22470 at 4624.651 kb on - strand, within ABIE41_RS22470 at 4624.862 kb on + strand, within ABIE41_RS22470 at 4624.862 kb on + strand, within ABIE41_RS22470 at 4624.863 kb on - strand, within ABIE41_RS22470 at 4624.863 kb on - strand, within ABIE41_RS22470 at 4625.112 kb on + strand, within ABIE41_RS22470 at 4625.112 kb on + strand, within ABIE41_RS22470 at 4625.112 kb on + strand, within ABIE41_RS22470 at 4625.113 kb on - strand, within ABIE41_RS22470 at 4625.157 kb on - strand, within ABIE41_RS22470 at 4625.157 kb on - strand, within ABIE41_RS22470 at 4625.157 kb on - strand, within ABIE41_RS22470 at 4625.189 kb on + strand, within ABIE41_RS22470 at 4625.189 kb on + strand, within ABIE41_RS22470 at 4625.189 kb on + strand, within ABIE41_RS22470 at 4625.190 kb on - strand, within ABIE41_RS22470 at 4625.190 kb on - strand, within ABIE41_RS22470 at 4625.219 kb on + strand, within ABIE41_RS22470 at 4625.219 kb on + strand, within ABIE41_RS22470 at 4625.219 kb on + strand, within ABIE41_RS22470 at 4625.219 kb on + strand, within ABIE41_RS22470 at 4625.219 kb on + strand, within ABIE41_RS22470 at 4625.220 kb on - strand, within ABIE41_RS22470 at 4625.220 kb on - strand, within ABIE41_RS22470 at 4625.220 kb on - strand, within ABIE41_RS22470 at 4625.220 kb on - strand, within ABIE41_RS22470 at 4625.220 kb on - strand, within ABIE41_RS22470 at 4625.225 kb on + strand, within ABIE41_RS22470 at 4625.226 kb on - strand, within ABIE41_RS22470 at 4625.420 kb on + strand, within ABIE41_RS22470 at 4625.420 kb on + strand, within ABIE41_RS22470 at 4625.421 kb on - strand, within ABIE41_RS22470 at 4625.486 kb on + strand, within ABIE41_RS22470 at 4625.486 kb on + strand, within ABIE41_RS22470 at 4625.486 kb on + strand, within ABIE41_RS22470 at 4625.534 kb on + strand, within ABIE41_RS22470 at 4625.843 kb on + strand, within ABIE41_RS22470 at 4625.844 kb on - strand, within ABIE41_RS22470 at 4625.844 kb on - strand, within ABIE41_RS22470 at 4625.876 kb on + strand, within ABIE41_RS22470 at 4625.919 kb on + strand at 4625.920 kb on - strand at 4625.920 kb on - strand at 4625.924 kb on + strand at 4625.925 kb on - strand at 4625.925 kb on - strand at 4626.065 kb on + strand at 4626.065 kb on + strand at 4626.211 kb on + strand at 4626.212 kb on - strand at 4626.253 kb on + strand at 4626.253 kb on + strand at 4626.254 kb on - strand at 4626.254 kb on - strand at 4626.283 kb on + strand at 4626.291 kb on + strand, within ABIE41_RS22475
Per-strain Table
Position Strand Gene LocusTag Fraction L-Proline (C) remove 4,623,290 + ABIE41_RS22465 0.16 -0.3 4,623,290 + ABIE41_RS22465 0.16 -1.5 4,623,290 + ABIE41_RS22465 0.16 -1.6 4,623,341 + ABIE41_RS22465 0.28 -1.2 4,623,362 + ABIE41_RS22465 0.33 -3.1 4,623,363 - ABIE41_RS22465 0.34 -0.3 4,623,363 - ABIE41_RS22465 0.34 +0.3 4,623,363 - ABIE41_RS22465 0.34 -3.4 4,623,581 + ABIE41_RS22465 0.86 -1.8 4,623,581 + ABIE41_RS22465 0.86 -1.1 4,623,582 - ABIE41_RS22465 0.86 -1.8 4,623,615 - +0.1 4,623,659 - +0.1 4,623,662 + -0.9 4,623,662 + -0.5 4,623,663 - -0.6 4,623,741 - -0.1 4,623,944 - +0.8 4,623,944 - -1.1 4,624,094 + -1.1 4,624,095 - -0.7 4,624,095 - -0.7 4,624,169 + +0.4 4,624,170 - +0.4 4,624,170 - -1.3 4,624,191 - +0.1 4,624,307 + ABIE41_RS22470 0.15 -0.0 4,624,307 + ABIE41_RS22470 0.15 -0.6 4,624,308 - ABIE41_RS22470 0.15 +0.7 4,624,310 + ABIE41_RS22470 0.15 +0.4 4,624,311 - ABIE41_RS22470 0.16 -1.1 4,624,311 - ABIE41_RS22470 0.16 -1.3 4,624,320 - ABIE41_RS22470 0.16 -1.7 4,624,430 + ABIE41_RS22470 0.21 -1.8 4,624,610 + ABIE41_RS22470 0.29 -0.7 4,624,610 + ABIE41_RS22470 0.29 -1.6 4,624,610 + ABIE41_RS22470 0.29 -0.7 4,624,610 + ABIE41_RS22470 0.29 +0.3 4,624,611 - ABIE41_RS22470 0.29 -2.2 4,624,650 + ABIE41_RS22470 0.31 -2.6 4,624,651 - ABIE41_RS22470 0.31 +0.9 4,624,862 + ABIE41_RS22470 0.41 -2.1 4,624,862 + ABIE41_RS22470 0.41 +0.1 4,624,863 - ABIE41_RS22470 0.41 +1.1 4,624,863 - ABIE41_RS22470 0.41 -3.3 4,625,112 + ABIE41_RS22470 0.53 -1.1 4,625,112 + ABIE41_RS22470 0.53 -0.1 4,625,112 + ABIE41_RS22470 0.53 -0.5 4,625,113 - ABIE41_RS22470 0.53 -0.9 4,625,157 - ABIE41_RS22470 0.55 -1.3 4,625,157 - ABIE41_RS22470 0.55 -0.7 4,625,157 - ABIE41_RS22470 0.55 -2.9 4,625,189 + ABIE41_RS22470 0.56 -0.6 4,625,189 + ABIE41_RS22470 0.56 -0.6 4,625,189 + ABIE41_RS22470 0.56 -0.3 4,625,190 - ABIE41_RS22470 0.56 +1.7 4,625,190 - ABIE41_RS22470 0.56 -0.1 4,625,219 + ABIE41_RS22470 0.58 +0.1 4,625,219 + ABIE41_RS22470 0.58 -0.6 4,625,219 + ABIE41_RS22470 0.58 +0.4 4,625,219 + ABIE41_RS22470 0.58 +0.7 4,625,219 + ABIE41_RS22470 0.58 +1.7 4,625,220 - ABIE41_RS22470 0.58 -0.3 4,625,220 - ABIE41_RS22470 0.58 -0.9 4,625,220 - ABIE41_RS22470 0.58 +2.7 4,625,220 - ABIE41_RS22470 0.58 +0.1 4,625,220 - ABIE41_RS22470 0.58 -1.3 4,625,225 + ABIE41_RS22470 0.58 -0.9 4,625,226 - ABIE41_RS22470 0.58 -1.1 4,625,420 + ABIE41_RS22470 0.67 -0.2 4,625,420 + ABIE41_RS22470 0.67 -1.1 4,625,421 - ABIE41_RS22470 0.67 -1.3 4,625,486 + ABIE41_RS22470 0.70 -0.3 4,625,486 + ABIE41_RS22470 0.70 -0.2 4,625,486 + ABIE41_RS22470 0.70 -0.7 4,625,534 + ABIE41_RS22470 0.72 +0.7 4,625,843 + ABIE41_RS22470 0.87 +0.6 4,625,844 - ABIE41_RS22470 0.87 +1.1 4,625,844 - ABIE41_RS22470 0.87 -1.0 4,625,876 + ABIE41_RS22470 0.88 +2.0 4,625,919 + +1.0 4,625,920 - -3.6 4,625,920 - +0.2 4,625,924 + +0.5 4,625,925 - +0.1 4,625,925 - +1.2 4,626,065 + +0.2 4,626,065 + +0.8 4,626,211 + -0.7 4,626,212 - +0.2 4,626,253 + -1.3 4,626,253 + -1.5 4,626,254 - -0.7 4,626,254 - +0.4 4,626,283 + -0.5 4,626,291 + ABIE41_RS22475 0.10 -3.3
Or see this region's nucleotide sequence