Strain Fitness in Escherichia coli BW25113 around b4122

Experiment: D-Serine (N)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntmelB and yjdF are separated by 138 nucleotidesyjdF and fumB are separated by 121 nucleotidesfumB and dcuB are separated by 77 nucleotides b4120: melB - melibiose:sodium symporter (NCBI), at 4,341,392 to 4,342,813 melB b4121: yjdF - conserved inner membrane protein (NCBI), at 4,342,952 to 4,343,581 yjdF b4122: fumB - anaerobic class I fumarate hydratase (fumarase B) (NCBI), at 4,343,703 to 4,345,349 fumB b4123: dcuB - C4-dicarboxylate antiporter (NCBI), at 4,345,427 to 4,346,767 dcuB Position (kb) 4343 4344 4345 4346Strain fitness (log2 ratio) -3 -2 -1 0 1 2 3at 4342.705 kb on - strandat 4342.718 kb on + strandat 4342.719 kb on - strandat 4342.719 kb on - strandat 4342.720 kb on - strandat 4342.773 kb on + strandat 4342.786 kb on - strandat 4342.786 kb on - strandat 4342.788 kb on + strandat 4342.797 kb on - strandat 4342.817 kb on + strandat 4342.831 kb on - strandat 4342.831 kb on - strandat 4342.913 kb on + strandat 4342.913 kb on + strandat 4342.937 kb on + strandat 4342.966 kb on + strandat 4343.050 kb on - strand, within yjdFat 4343.151 kb on + strand, within yjdFat 4343.169 kb on - strand, within yjdFat 4343.182 kb on - strand, within yjdFat 4343.182 kb on - strand, within yjdFat 4343.242 kb on + strand, within yjdFat 4343.268 kb on - strand, within yjdFat 4343.289 kb on + strand, within yjdFat 4343.292 kb on + strand, within yjdFat 4343.292 kb on + strand, within yjdFat 4343.299 kb on + strand, within yjdFat 4343.313 kb on - strand, within yjdFat 4343.313 kb on - strand, within yjdFat 4343.376 kb on - strand, within yjdFat 4343.399 kb on + strand, within yjdFat 4343.402 kb on - strand, within yjdFat 4343.516 kb on + strand, within yjdFat 4343.516 kb on + strand, within yjdFat 4343.527 kb on + strandat 4343.546 kb on + strandat 4343.547 kb on + strandat 4343.547 kb on + strandat 4343.579 kb on + strandat 4343.579 kb on + strandat 4343.666 kb on + strandat 4343.684 kb on - strandat 4343.700 kb on + strandat 4343.715 kb on + strandat 4343.815 kb on + strandat 4343.826 kb on - strandat 4343.836 kb on - strandat 4343.836 kb on - strandat 4343.868 kb on - strand, within fumBat 4343.942 kb on + strand, within fumBat 4343.980 kb on - strand, within fumBat 4343.980 kb on - strand, within fumBat 4344.047 kb on + strand, within fumBat 4344.047 kb on + strand, within fumBat 4344.056 kb on + strand, within fumBat 4344.099 kb on - strand, within fumBat 4344.103 kb on + strand, within fumBat 4344.193 kb on + strand, within fumBat 4344.206 kb on - strand, within fumBat 4344.206 kb on - strand, within fumBat 4344.320 kb on + strand, within fumBat 4344.320 kb on + strand, within fumBat 4344.466 kb on + strand, within fumBat 4344.466 kb on + strand, within fumBat 4344.609 kb on - strand, within fumBat 4344.677 kb on - strand, within fumBat 4344.757 kb on + strand, within fumBat 4344.868 kb on - strand, within fumBat 4344.871 kb on + strand, within fumBat 4344.888 kb on - strand, within fumBat 4344.923 kb on - strand, within fumBat 4344.924 kb on + strand, within fumBat 4344.945 kb on - strand, within fumBat 4344.945 kb on - strand, within fumBat 4344.945 kb on - strand, within fumBat 4345.028 kb on + strand, within fumBat 4345.028 kb on + strand, within fumBat 4345.098 kb on - strand, within fumBat 4345.098 kb on - strand, within fumBat 4345.098 kb on - strand, within fumBat 4345.179 kb on - strand, within fumBat 4345.181 kb on + strand, within fumBat 4345.181 kb on + strand, within fumBat 4345.276 kb on - strandat 4345.289 kb on + strandat 4345.289 kb on + strandat 4345.294 kb on + strandat 4345.302 kb on - strandat 4345.358 kb on - strandat 4345.358 kb on - strandat 4345.368 kb on - strandat 4345.368 kb on - strandat 4345.392 kb on + strandat 4345.402 kb on + strandat 4345.444 kb on - strandat 4345.444 kb on - strandat 4345.461 kb on - strandat 4345.461 kb on - strandat 4345.472 kb on + strandat 4345.486 kb on - strandat 4345.486 kb on - strandat 4345.516 kb on - strandat 4345.532 kb on + strandat 4345.550 kb on - strandat 4345.608 kb on + strand, within dcuBat 4345.687 kb on + strand, within dcuBat 4345.687 kb on + strand, within dcuBat 4345.708 kb on - strand, within dcuBat 4345.708 kb on - strand, within dcuBat 4345.766 kb on + strand, within dcuBat 4345.835 kb on - strand, within dcuBat 4346.100 kb on + strand, within dcuBat 4346.100 kb on + strand, within dcuBat 4346.160 kb on + strand, within dcuBat 4346.160 kb on + strand, within dcuBat 4346.207 kb on - strand, within dcuBat 4346.207 kb on - strand, within dcuBat 4346.281 kb on - strand, within dcuBat 4346.281 kb on - strand, within dcuBat 4346.283 kb on + strand, within dcuBat 4346.320 kb on - strand, within dcuBat 4346.320 kb on - strand, within dcuB

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Per-strain Table

Position Strand Gene LocusTag Fraction D-Serine (N)
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4,342,705 - +0.3
4,342,718 + -0.9
4,342,719 - +0.2
4,342,719 - +0.2
4,342,720 - +1.2
4,342,773 + +0.9
4,342,786 - -0.4
4,342,786 - +0.5
4,342,788 + -0.6
4,342,797 - -0.9
4,342,817 + +0.5
4,342,831 - -0.7
4,342,831 - +0.2
4,342,913 + -1.1
4,342,913 + -0.3
4,342,937 + +0.2
4,342,966 + -0.6
4,343,050 - yjdF b4121 0.16 -0.7
4,343,151 + yjdF b4121 0.32 +0.0
4,343,169 - yjdF b4121 0.34 -0.9
4,343,182 - yjdF b4121 0.37 +0.6
4,343,182 - yjdF b4121 0.37 +0.0
4,343,242 + yjdF b4121 0.46 -0.6
4,343,268 - yjdF b4121 0.50 -0.4
4,343,289 + yjdF b4121 0.53 +0.7
4,343,292 + yjdF b4121 0.54 -0.0
4,343,292 + yjdF b4121 0.54 -0.1
4,343,299 + yjdF b4121 0.55 -0.4
4,343,313 - yjdF b4121 0.57 -0.3
4,343,313 - yjdF b4121 0.57 -0.5
4,343,376 - yjdF b4121 0.67 -3.1
4,343,399 + yjdF b4121 0.71 +0.3
4,343,402 - yjdF b4121 0.71 +0.1
4,343,516 + yjdF b4121 0.90 -0.1
4,343,516 + yjdF b4121 0.90 +1.4
4,343,527 + +0.0
4,343,546 + +0.0
4,343,547 + -0.0
4,343,547 + -1.0
4,343,579 + +0.3
4,343,579 + -0.6
4,343,666 + +0.5
4,343,684 - -0.2
4,343,700 + +0.9
4,343,715 + -0.3
4,343,815 + +0.2
4,343,826 - -0.6
4,343,836 - -1.3
4,343,836 - +0.6
4,343,868 - fumB b4122 0.10 -1.6
4,343,942 + fumB b4122 0.15 -0.7
4,343,980 - fumB b4122 0.17 -0.5
4,343,980 - fumB b4122 0.17 -0.2
4,344,047 + fumB b4122 0.21 +0.8
4,344,047 + fumB b4122 0.21 -0.4
4,344,056 + fumB b4122 0.21 +0.1
4,344,099 - fumB b4122 0.24 -0.2
4,344,103 + fumB b4122 0.24 -0.1
4,344,193 + fumB b4122 0.30 +0.0
4,344,206 - fumB b4122 0.31 +0.2
4,344,206 - fumB b4122 0.31 +1.2
4,344,320 + fumB b4122 0.37 +0.1
4,344,320 + fumB b4122 0.37 +0.5
4,344,466 + fumB b4122 0.46 +0.7
4,344,466 + fumB b4122 0.46 +1.2
4,344,609 - fumB b4122 0.55 -0.2
4,344,677 - fumB b4122 0.59 +0.5
4,344,757 + fumB b4122 0.64 -0.3
4,344,868 - fumB b4122 0.71 -0.3
4,344,871 + fumB b4122 0.71 +0.3
4,344,888 - fumB b4122 0.72 -0.8
4,344,923 - fumB b4122 0.74 +0.2
4,344,924 + fumB b4122 0.74 -0.3
4,344,945 - fumB b4122 0.75 +0.3
4,344,945 - fumB b4122 0.75 -0.9
4,344,945 - fumB b4122 0.75 -1.7
4,345,028 + fumB b4122 0.80 -0.6
4,345,028 + fumB b4122 0.80 -2.0
4,345,098 - fumB b4122 0.85 +0.1
4,345,098 - fumB b4122 0.85 -2.0
4,345,098 - fumB b4122 0.85 -0.8
4,345,179 - fumB b4122 0.90 +0.5
4,345,181 + fumB b4122 0.90 +0.5
4,345,181 + fumB b4122 0.90 +1.8
4,345,276 - -0.1
4,345,289 + -0.2
4,345,289 + +0.7
4,345,294 + +0.6
4,345,302 - -0.2
4,345,358 - +0.7
4,345,358 - -0.2
4,345,368 - +0.6
4,345,368 - +0.3
4,345,392 + -0.2
4,345,402 + +0.7
4,345,444 - +0.0
4,345,444 - +0.7
4,345,461 - -0.2
4,345,461 - -0.1
4,345,472 + +0.6
4,345,486 - +0.6
4,345,486 - +0.2
4,345,516 - -0.1
4,345,532 + +0.1
4,345,550 - -0.1
4,345,608 + dcuB b4123 0.13 -0.5
4,345,687 + dcuB b4123 0.19 +0.6
4,345,687 + dcuB b4123 0.19 -0.8
4,345,708 - dcuB b4123 0.21 -0.3
4,345,708 - dcuB b4123 0.21 -1.7
4,345,766 + dcuB b4123 0.25 +0.6
4,345,835 - dcuB b4123 0.30 -0.1
4,346,100 + dcuB b4123 0.50 -0.9
4,346,100 + dcuB b4123 0.50 -0.1
4,346,160 + dcuB b4123 0.55 +0.5
4,346,160 + dcuB b4123 0.55 -0.4
4,346,207 - dcuB b4123 0.58 +0.0
4,346,207 - dcuB b4123 0.58 +0.0
4,346,281 - dcuB b4123 0.64 +0.6
4,346,281 - dcuB b4123 0.64 +2.8
4,346,283 + dcuB b4123 0.64 -0.3
4,346,320 - dcuB b4123 0.67 -0.6
4,346,320 - dcuB b4123 0.67 +0.9

Or see this region's nucleotide sequence