Experiment: Acetate Carbon Source (20mM)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_2337 and PP_2338 are separated by 166 nucleotides PP_2338 and PP_2339 are separated by 74 nucleotides
PP_2337: PP_2337 - aconitate isomerase, at 2,666,792 to 2,667,982
_2337
PP_2338: PP_2338 - 2-methylcitrate dehydratase, at 2,668,149 to 2,669,633
_2338
PP_2339: PP_2339 - bifunctional aconitate hydratase 2 and 2-methylisocitrate dehydratase, at 2,669,708 to 2,672,317
_2339
Position (kb)
2668
2669
2670 Strain fitness (log2 ratio)
-1
0
1 at 2667.196 kb on + strand, within PP_2337 at 2667.196 kb on + strand, within PP_2337 at 2667.416 kb on - strand, within PP_2337 at 2667.482 kb on + strand, within PP_2337 at 2667.483 kb on - strand, within PP_2337 at 2667.580 kb on + strand, within PP_2337 at 2667.653 kb on + strand, within PP_2337 at 2667.653 kb on + strand, within PP_2337 at 2668.053 kb on + strand at 2668.054 kb on - strand at 2668.079 kb on - strand at 2668.162 kb on + strand at 2668.163 kb on - strand at 2668.163 kb on - strand at 2668.163 kb on - strand at 2668.198 kb on + strand at 2668.199 kb on - strand at 2668.199 kb on - strand at 2668.216 kb on - strand at 2668.392 kb on + strand, within PP_2338 at 2668.452 kb on + strand, within PP_2338 at 2668.779 kb on - strand, within PP_2338 at 2668.779 kb on - strand, within PP_2338 at 2668.923 kb on + strand at 2668.923 kb on + strand, within PP_2338 at 2668.923 kb on + strand, within PP_2338 at 2668.923 kb on + strand, within PP_2338 at 2668.923 kb on + strand, within PP_2338 at 2668.924 kb on - strand, within PP_2338 at 2668.924 kb on - strand, within PP_2338 at 2668.944 kb on - strand, within PP_2338 at 2669.013 kb on + strand, within PP_2338 at 2669.013 kb on + strand, within PP_2338 at 2669.013 kb on + strand, within PP_2338 at 2669.014 kb on - strand, within PP_2338 at 2669.014 kb on - strand, within PP_2338 at 2669.090 kb on - strand, within PP_2338 at 2669.090 kb on - strand, within PP_2338 at 2669.111 kb on + strand, within PP_2338 at 2669.111 kb on + strand, within PP_2338 at 2669.226 kb on + strand, within PP_2338 at 2669.226 kb on + strand, within PP_2338 at 2669.226 kb on + strand at 2669.226 kb on + strand, within PP_2338 at 2669.226 kb on + strand, within PP_2338 at 2669.227 kb on - strand, within PP_2338 at 2669.227 kb on - strand, within PP_2338 at 2669.227 kb on - strand, within PP_2338 at 2669.227 kb on - strand, within PP_2338 at 2669.274 kb on + strand, within PP_2338 at 2669.274 kb on + strand, within PP_2338 at 2669.274 kb on + strand, within PP_2338 at 2669.489 kb on - strand at 2669.709 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Acetate Carbon Source (20mM) remove 2,667,196 + PP_2337 0.34 +0.0 2,667,196 + PP_2337 0.34 -0.5 2,667,416 - PP_2337 0.52 +1.2 2,667,482 + PP_2337 0.58 +0.9 2,667,483 - PP_2337 0.58 +0.5 2,667,580 + PP_2337 0.66 -0.7 2,667,653 + PP_2337 0.72 -0.3 2,667,653 + PP_2337 0.72 +1.3 2,668,053 + +0.7 2,668,054 - -0.5 2,668,079 - -0.1 2,668,162 + -0.0 2,668,163 - -0.5 2,668,163 - -0.7 2,668,163 - -0.5 2,668,198 + -0.4 2,668,199 - -0.2 2,668,199 - +0.0 2,668,216 - -1.0 2,668,392 + PP_2338 0.16 +1.2 2,668,452 + PP_2338 0.20 -0.7 2,668,779 - PP_2338 0.42 -0.8 2,668,779 - PP_2338 0.42 -0.5 2,668,923 + +1.1 2,668,923 + PP_2338 0.52 -0.6 2,668,923 + PP_2338 0.52 +0.5 2,668,923 + PP_2338 0.52 -0.4 2,668,923 + PP_2338 0.52 +0.8 2,668,924 - PP_2338 0.52 +0.2 2,668,924 - PP_2338 0.52 +1.3 2,668,944 - PP_2338 0.54 -0.2 2,669,013 + PP_2338 0.58 -0.2 2,669,013 + PP_2338 0.58 -1.7 2,669,013 + PP_2338 0.58 -0.5 2,669,014 - PP_2338 0.58 +0.5 2,669,014 - PP_2338 0.58 +0.5 2,669,090 - PP_2338 0.63 +0.3 2,669,090 - PP_2338 0.63 +0.2 2,669,111 + PP_2338 0.65 -1.1 2,669,111 + PP_2338 0.65 -0.0 2,669,226 + PP_2338 0.73 +1.1 2,669,226 + PP_2338 0.73 -0.3 2,669,226 + +0.5 2,669,226 + PP_2338 0.73 +0.7 2,669,226 + PP_2338 0.73 +0.7 2,669,227 - PP_2338 0.73 -0.5 2,669,227 - PP_2338 0.73 +0.5 2,669,227 - PP_2338 0.73 +1.2 2,669,227 - PP_2338 0.73 -0.5 2,669,274 + PP_2338 0.76 +0.3 2,669,274 + PP_2338 0.76 -0.8 2,669,274 + PP_2338 0.76 +0.5 2,669,489 - +0.1 2,669,709 + -1.7
Or see this region's nucleotide sequence