Experiment: LB_plus_SM_buffer with FelixO1_phage 0.05 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF904 and GFF905 are separated by 98 nucleotides GFF905 and GFF906 are separated by 3 nucleotides GFF906 and GFF907 are separated by 160 nucleotides GFF907 and GFF908 are separated by 10 nucleotides GFF908 and GFF909 overlap by 1 nucleotides
GFF904 - TldE protein, part of TldE/TldD proteolytic complex, at 85,190 to 86,530
GFF904
GFF905 - Soluble cytochrome b562, at 86,629 to 87,015
GFF905
GFF906 - FIG01047835: hypothetical protein, at 87,019 to 87,141
GFF906
GFF907 - FIG01045571: hypothetical protein, at 87,302 to 87,631
GFF907
GFF908 - FIG00628496: hypothetical protein, at 87,642 to 88,004
GFF908
GFF909 - FIG074102: hypothetical protein, at 88,004 to 88,306
GFF909
Position (kb) 87
88 Strain fitness (log2 ratio) -2
-1
0
1 at 86.171 kb on + strand, within GFF904 at 86.171 kb on + strand, within GFF904 at 86.339 kb on + strand, within GFF904 at 86.340 kb on - strand, within GFF904 at 86.405 kb on - strand at 86.440 kb on + strand at 86.479 kb on + strand at 86.481 kb on + strand at 86.481 kb on + strand at 86.599 kb on + strand at 86.645 kb on + strand at 86.709 kb on + strand, within GFF905 at 86.713 kb on - strand, within GFF905 at 86.991 kb on + strand at 86.991 kb on + strand at 86.992 kb on - strand at 87.000 kb on + strand at 87.001 kb on - strand at 87.001 kb on - strand at 87.001 kb on - strand at 87.007 kb on + strand at 87.070 kb on - strand, within GFF906 at 87.202 kb on - strand at 87.273 kb on - strand at 87.366 kb on + strand, within GFF907 at 87.469 kb on + strand, within GFF907 at 87.629 kb on + strand at 87.653 kb on + strand at 87.834 kb on - strand, within GFF908 at 87.859 kb on - strand, within GFF908 at 87.948 kb on - strand, within GFF908 at 88.038 kb on + strand, within GFF909 at 88.038 kb on + strand, within GFF909 at 88.123 kb on + strand, within GFF909 at 88.128 kb on + strand, within GFF909 at 88.128 kb on + strand, within GFF909 at 88.136 kb on - strand, within GFF909
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with FelixO1_phage 0.05 MOI remove 86,171 + GFF904 0.73 +0.3 86,171 + GFF904 0.73 -0.6 86,339 + GFF904 0.86 -1.3 86,340 - GFF904 0.86 +0.2 86,405 - -0.8 86,440 + -0.6 86,479 + -0.2 86,481 + -0.3 86,481 + -0.4 86,599 + +0.5 86,645 + -0.5 86,709 + GFF905 0.21 -1.9 86,713 - GFF905 0.22 -0.3 86,991 + -0.3 86,991 + +0.8 86,992 - +0.6 87,000 + -0.1 87,001 - -0.1 87,001 - -0.2 87,001 - +0.1 87,007 + -0.9 87,070 - GFF906 0.41 -1.1 87,202 - -0.1 87,273 - -0.3 87,366 + GFF907 0.19 -0.2 87,469 + GFF907 0.51 +0.1 87,629 + +0.1 87,653 + +0.3 87,834 - GFF908 0.53 +0.4 87,859 - GFF908 0.60 -0.1 87,948 - GFF908 0.84 -2.2 88,038 + GFF909 0.11 -0.4 88,038 + GFF909 0.11 -0.6 88,123 + GFF909 0.39 +0.5 88,128 + GFF909 0.41 -0.7 88,128 + GFF909 0.41 -0.4 88,136 - GFF909 0.44 +1.5
Or see this region's nucleotide sequence