Experiment: LB_plus_SM_buffer with FelixO1_phage 0.05 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF728 and GFF729 are separated by 60 nucleotides GFF729 and GFF730 are separated by 2 nucleotides GFF730 and GFF731 are separated by 90 nucleotides
GFF728 - Dipeptide transport system, periplasmic component in protein degradation cluster, at 87,069 to 88,607
GFF728
GFF729 - Dipeptide transport system permease protein DppB in protein degradation cluster, at 88,668 to 89,588
GFF729
GFF730 - Dipeptide transport system permease protein DppC (TC 3.A.1.5.2), at 89,591 to 90,502
GFF730
GFF731 - Ribosomal protein S12p Asp88 (E. coli) methylthiotransferase, at 90,593 to 91,918
GFF731
Position (kb)
89
90
91 Strain fitness (log2 ratio)
-1
0
1
2 at 88.605 kb on + strand at 88.605 kb on + strand at 88.606 kb on - strand at 88.606 kb on - strand at 88.609 kb on + strand at 88.610 kb on - strand at 88.677 kb on + strand at 88.678 kb on - strand at 88.702 kb on + strand at 88.709 kb on + strand at 88.983 kb on - strand, within GFF729 at 88.983 kb on - strand, within GFF729 at 88.987 kb on + strand, within GFF729 at 89.278 kb on - strand, within GFF729 at 89.326 kb on + strand, within GFF729 at 89.426 kb on + strand, within GFF729 at 89.582 kb on + strand at 89.583 kb on - strand at 89.586 kb on + strand at 89.586 kb on + strand at 89.586 kb on + strand at 89.643 kb on + strand at 89.975 kb on + strand, within GFF730 at 89.975 kb on + strand, within GFF730 at 89.998 kb on + strand, within GFF730 at 90.148 kb on + strand, within GFF730 at 90.148 kb on + strand, within GFF730 at 90.176 kb on - strand, within GFF730 at 90.230 kb on - strand, within GFF730 at 90.231 kb on + strand, within GFF730 at 90.232 kb on - strand, within GFF730 at 90.232 kb on - strand, within GFF730 at 90.687 kb on + strand at 90.724 kb on + strand at 90.830 kb on - strand, within GFF731 at 91.020 kb on - strand, within GFF731 at 91.054 kb on + strand, within GFF731 at 91.210 kb on - strand, within GFF731 at 91.317 kb on - strand, within GFF731 at 91.333 kb on + strand, within GFF731 at 91.334 kb on - strand, within GFF731 at 91.395 kb on - strand, within GFF731 at 91.497 kb on + strand, within GFF731
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with FelixO1_phage 0.05 MOI remove 88,605 + -0.8 88,605 + +0.5 88,606 - +1.0 88,606 - +1.0 88,609 + +0.0 88,610 - +0.3 88,677 + +0.3 88,678 - +0.6 88,702 + +0.5 88,709 + +0.4 88,983 - GFF729 0.34 +0.7 88,983 - GFF729 0.34 +0.9 88,987 + GFF729 0.35 +0.6 89,278 - GFF729 0.66 +1.3 89,326 + GFF729 0.71 -0.0 89,426 + GFF729 0.82 +0.2 89,582 + -0.6 89,583 - +0.8 89,586 + -0.5 89,586 + +0.3 89,586 + +0.7 89,643 + +0.7 89,975 + GFF730 0.42 +0.7 89,975 + GFF730 0.42 +0.3 89,998 + GFF730 0.45 -0.1 90,148 + GFF730 0.61 +1.2 90,148 + GFF730 0.61 +1.2 90,176 - GFF730 0.64 +0.7 90,230 - GFF730 0.70 +0.2 90,231 + GFF730 0.70 +2.3 90,232 - GFF730 0.70 +0.1 90,232 - GFF730 0.70 -0.1 90,687 + +0.4 90,724 + -0.1 90,830 - GFF731 0.18 -0.7 91,020 - GFF731 0.32 +0.2 91,054 + GFF731 0.35 +1.8 91,210 - GFF731 0.47 +0.1 91,317 - GFF731 0.55 -0.7 91,333 + GFF731 0.56 +1.1 91,334 - GFF731 0.56 +0.5 91,395 - GFF731 0.60 +0.2 91,497 + GFF731 0.68 -1.0
Or see this region's nucleotide sequence