Strain Fitness in Salmonella enterica subsp. enterica serovar Typhimurium str. MS1868 around GFF4832

Experiment: LB_plus_SM_buffer with FelixO1_phage 0.05 MOI

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF4830 and GFF4831 are separated by 30 nucleotidesGFF4831 and GFF4832 are separated by 135 nucleotidesGFF4832 and GFF4833 are separated by 82 nucleotides GFF4830 - hypothetical protein, at 126,346 to 126,492 GFF4830 GFF4831 - Queuosine Biosynthesis QueE Radical SAM, at 126,523 to 127,194 GFF4831 GFF4832 - Enolase (EC 4.2.1.11), at 127,330 to 128,628 GFF4832 GFF4833 - CTP synthase (EC 6.3.4.2), at 128,711 to 130,348 GFF4833 Position (kb) 127 128 129Strain fitness (log2 ratio) -5 -4 -3 -2 -1 0 1at 126.469 kb on + strand, within GFF4830at 126.516 kb on + strandat 126.591 kb on + strand, within GFF4831at 126.610 kb on + strand, within GFF4831at 126.621 kb on + strand, within GFF4831at 126.813 kb on + strand, within GFF4831at 126.814 kb on - strand, within GFF4831at 126.856 kb on - strand, within GFF4831at 126.945 kb on - strand, within GFF4831at 127.075 kb on - strand, within GFF4831at 127.075 kb on - strand, within GFF4831at 127.078 kb on - strand, within GFF4831at 127.146 kb on - strandat 127.216 kb on - strandat 127.226 kb on + strandat 127.280 kb on - strandat 127.307 kb on + strandat 127.307 kb on + strandat 127.354 kb on - strandat 127.364 kb on + strandat 127.364 kb on + strandat 128.653 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with FelixO1_phage 0.05 MOI
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126,469 + GFF4830 0.84 +0.2
126,516 + +0.3
126,591 + GFF4831 0.10 -0.0
126,610 + GFF4831 0.13 +0.2
126,621 + GFF4831 0.15 -0.1
126,813 + GFF4831 0.43 -2.3
126,814 - GFF4831 0.43 +1.0
126,856 - GFF4831 0.50 +0.3
126,945 - GFF4831 0.63 +1.6
127,075 - GFF4831 0.82 -0.2
127,075 - GFF4831 0.82 +0.7
127,078 - GFF4831 0.83 -0.2
127,146 - +0.1
127,216 - -0.4
127,226 + +0.0
127,280 - +0.2
127,307 + -1.0
127,307 + -5.0
127,354 - -1.5
127,364 + -0.3
127,364 + -3.5
128,653 - -1.0

Or see this region's nucleotide sequence